Product nameAnti-Aldolase B antibody [EPR3138Y]
See all Aldolase B primary antibodies
DescriptionRabbit monoclonal [EPR3138Y] to Aldolase B
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Unsuitable for: ICC or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Aldolase B aa 250-350. The exact sequence is proprietary.
- Fetal kidney lysate. Human liver tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab75751 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 39 kDa.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
PathwayCarbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.
Involvement in diseaseDefects in ALDOB are the cause of hereditary fructose intolerance (HFI) [MIM:229600]. HFI is an autosomal recessive disease that results in an inability to metabolize fructose and related sugars. Complete exclusion of fructose results in dramatic recovery; however, if not treated properly, HFI subjects suffer episodes of hypoglycemia, general ill condition, and risk of death the remainder of life.
Sequence similaritiesBelongs to the class I fructose-bisphosphate aldolase family.
- Information by UniProt
- ALDB antibody
- ALDO B antibody
- ALDO2 antibody
Anti-Aldolase B antibody [EPR3138Y] (ab75751) at 1/1000 dilution + fetal kidney lysate at 10 µg
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDa
ab75751, at 1/100 dilution, staining Aldolase B in paraffin-embedded human liver tissue.
ab75751 showing positive staining in Normal kidney tissue.
ab75751 showing negative staining in Normal colon tissue.
ab75751 showing negative staining in Normal lung tissue.
ab75751 showing negative staining in Normal tonsil tissue.
This product has been referenced in:
- Wang C et al. Long-term supranutritional supplementation with selenate decreases hyperglycemia and promotes fatty liver degeneration by inducing hyperinsulinemia in diabetic db/db mice. PLoS One 9:e101315 (2014). WB . Read more (PubMed: 24983750) »
- Tun HW et al. Pathway signature and cellular differentiation in clear cell renal cell carcinoma. PLoS One 5:e10696 (2010). IHC-P ; Human . Read more (PubMed: 20502531) »