Recombinant Anti-Aldolase B + Aldolase C antibody [EPR3137] (ab133333)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3137] to Aldolase B + Aldolase C
- Suitable for: IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Aldolase B + Aldolase C antibody [EPR3137]
See all Aldolase B+Aldolase C primary antibodies -
Description
Rabbit monoclonal [EPR3137] to Aldolase B + Aldolase C -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human Aldolase B aa 100-200. The exact sequence is proprietary.
Database link: P05062 -
Positive control
- Human fetal liver and kidney cell lysates; Human fetal liver tissue; Human adult liver tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3137 -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133333 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/1000 - 1/2000. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa).
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Notes |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/1000 - 1/2000. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa). |
Images
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All lanes : Anti-Aldolase B + Aldolase C antibody [EPR3137] (ab133333) at 1/1000 dilution
Lane 1 : Human Aldolase A full-length recombinant protein with His-Tag
Lane 2 : Human Aldolase B full-length recombinant protein with His-Tag
Lane 3 : Human Aldolase C full-length recombinant protein with His-Tag
Lysates/proteins at 0.015 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 39 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondBlocking and Diluting buffer and concentration: 5% NFDM /TBST.
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Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human fetal liver tissue staining Aldolase B + Aldolase C with ab133333 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human adult liver tissue staining Aldolase B + Aldolase C with ab133333 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-Aldolase B + Aldolase C antibody [EPR3137] (ab133333) at 1/1000 dilution
Lane 1 : Human fetal liver cell lysate
Lane 2 : Human fetal kidney cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-rabbit HRP at 1/2000 dilution
Predicted band size: 39 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using ab133333 showing -ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded normal Human breast tissue using ab133333 showing -ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded normal Human kidney tissue using ab133333 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab133333 has been referenced in 4 publications.
- Chang YC et al. Enrichment of Aldolase C Correlates with Low Non-Mutated IDH1 Expression and Predicts a Favorable Prognosis in Glioblastomas. Cancers (Basel) 11:N/A (2019). PubMed: 31450822
- Tian YF et al. High Expression of Aldolase B Confers a Poor Prognosis for Rectal Cancer Patients Receiving Neoadjuvant Chemoradiotherapy. J Cancer 8:1197-1204 (2017). IHC-P ; Human . PubMed: 28607594
- Li Q et al. Aldolase B Overexpression is Associated with Poor Prognosis and Promotes Tumor Progression by Epithelial-Mesenchymal Transition in Colorectal Adenocarcinoma. Cell Physiol Biochem 42:397-406 (2017). PubMed: 28558381
- Liu Y et al. The role of von Willebrand factor as a biomarker of tumor development in hepatitis B virus-associated human hepatocellular carcinoma: a quantitative proteomic based study. J Proteomics 106:99-112 (2014). WB, IHC ; Human . PubMed: 24769235