Overview

  • Product name

    Anti-Aldolase B+Aldolase C antibody [EPR3137]
    See all Aldolase B+Aldolase C primary antibodies
  • Description

    Rabbit monoclonal [EPR3137] to Aldolase B+Aldolase C
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Aldolase B aa 100-200. The exact sequence is proprietary.
    Database link: P05062

  • Positive control

    • Human fetal liver and kidney cell lysates; Human fetal liver tissue; Human adult liver tissue.
  • General notes

     

     

     This product was previously labelled as ALDOB+Aldolase C

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133333 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/2000. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Images

    • All lanes : Anti-Aldolase B+Aldolase C antibody [EPR3137] (ab133333) at 1/1000 dilution

      Lane 1 : Human Aldolase A full-length recombinant protein with His-Tag
      Lane 2 : Human Aldolase B full-length recombinant protein with His-Tag
      Lane 3 : Human Aldolase C full-length recombinant protein with His-Tag

      Lysates/proteins at 0.015 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 39 kDa
      Observed band size: 40 kDa
      why is the actual band size different from the predicted?


      Exposure time: 1 second


      Blocking and Diluting buffer and concentration: 5% NFDM /TBST.

    • Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human fetal liver tissue staining Aldolase B + Aldolase C with ab133333 at 1/100 dilution.

    • Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human adult liver tissue staining Aldolase B + Aldolase C with ab133333 at 1/100 dilution.

    • All lanes : Anti-Aldolase B+Aldolase C antibody [EPR3137] (ab133333) at 1/1000 dilution

      Lane 1 : Human fetal liver cell lysate
      Lane 2 : Human fetal kidney cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 39 kDa
      Observed band size: 40 kDa why is the actual band size different from the predicted?

    • Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using ab133333 showing -ve staining.

    • Immunohistochemical analysis of paraffin embedded normal Human breast tissue using ab133333 showing -ve staining.

    • Immunohistochemical analysis of paraffin embedded normal Human kidney tissue using ab133333 showing +ve staining.

    References

    This product has been referenced in:

    • Tian YF  et al. High Expression of Aldolase B Confers a Poor Prognosis for Rectal Cancer Patients Receiving Neoadjuvant Chemoradiotherapy. J Cancer 8:1197-1204 (2017). IHC-P ; Human . Read more (PubMed: 28607594) »
    • Li Q  et al. Aldolase B Overexpression is Associated with Poor Prognosis and Promotes Tumor Progression by Epithelial-Mesenchymal Transition in Colorectal Adenocarcinoma. Cell Physiol Biochem 42:397-406 (2017). Read more (PubMed: 28558381) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab133333.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up