Anti-Aldolase C antibody [4A9] - N-terminal (ab190368)
Key features and details
- Mouse monoclonal [4A9] to Aldolase C - N-terminal
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Cow, Pig
- Isotype: IgG1
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Aldolase C antibody [4A9] - N-terminal
See all Aldolase C primary antibodies -
Description
Mouse monoclonal [4A9] to Aldolase C - N-terminal -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Cow, Pig
Predicted to work with: Rabbit, Horse, Chicken, Human, Chimpanzee, Cynomolgus monkey -
Immunogen
Synthetic peptide corresponding to Human Aldolase C aa 1-100 (N terminal).
Database link: P09972 -
Positive control
- WB: Rat and mouse brain lysate; Cow cerebellum lysate; Pig hippocampus lysate. ICC/IF: Rat cerebellum section and E20 rat cortical culture.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.07% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
4A9 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab190368 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/1000.
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WB |
1/2000. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa).
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Notes |
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ICC/IF
1/1000. |
WB
1/2000. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa). |
Target
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Pathway
Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4. -
Sequence similarities
Belongs to the class I fructose-bisphosphate aldolase family. - Information by UniProt
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Database links
- Entrez Gene: 395492 Chicken
- Entrez Gene: 493951 Chimpanzee
- Entrez Gene: 504584 Cow
- Entrez Gene: 101865220 Cynomolgus monkey
- Entrez Gene: 100059231 Horse
- Entrez Gene: 230 Human
- Entrez Gene: 11676 Mouse
- Entrez Gene: 100512013 Pig
see all -
Alternative names
- ALDC antibody
- ALDO C antibody
- aldoc antibody
see all
Images
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Immunofluorescence analysis of neuron/glial cells, labeling Aldolase C using ab190368 at a 1/500 dilution (green). Counterstained with an antibody against NeuN (red) and DNA (blue).
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All lanes : Anti-Aldolase C antibody [4A9] - N-terminal (ab190368) at 1/2000 dilution
Lane 1 : Protein standard
Lane 2 : Rat brain lysate
Lane 3 : Mouse brain lysate
Lane 4 : Cow cerebellum lysate
Lane 5 : Pig hippocampus lysate
Predicted band size: 39 kDa -
Immunofluorescence analysis of rat cerebellum tissue (A) or E20 rat cortical culture (B) stained for Aldolase C using ab190368 at a 1/1000 dilution (green)). Costained with rabbit polyclonal to CFAP (red) at a 1/5000 dilution (A) or with chicken polyclonal antibody to MAP2 (red) at a 1/10000 dilution (B). DAPI was used to staing nuclear DNA (blue).
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab190368 has been referenced in 1 publication.
- Toscano Márquez B et al. Molecular Identity and Location Influence Purkinje Cell Vulnerability in Autosomal-Recessive Spastic Ataxia of Charlevoix-Saguenay Mice. Front Cell Neurosci 15:707857 (2021). PubMed: 34970120