Overview

  • Product name

    Anti-Aldolase C antibody [4A9] - N-terminal
    See all Aldolase C primary antibodies
  • Description

    Mouse monoclonal [4A9] to Aldolase C - N-terminal
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Pig
    Predicted to work with: Rabbit, Horse, Chicken, Human, Chimpanzee, Cynomolgus monkey
  • Immunogen

    Synthetic peptide corresponding to Human Aldolase C aa 1-20 (N terminal).
    Sequence:

    MPHSYPALSAEQKKELSDIA


    Database link: P09972

  • Positive control

    • WB: Rat and mouse brain lysate; Cow cerebellum lysate; Pig hippocampus lysate. ICC/IF: Rat cerebellum section and E20 rat cortical culture.

Properties

Applications

Our Abpromise guarantee covers the use of ab190368 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000.
WB 1/2000. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa).

Target

Images

  • Immunofluorescence analysis of neuron/glial cells, labeling Aldolase C using ab190368 at a 1/500 dilution (green). Counterstained with an antibody against NeuN (red) and DNA (blue).

  • All lanes : Anti-Aldolase C antibody [4A9] - N-terminal (ab190368) at 1/2000 dilution

    Lane 1 : Protein standard
    Lane 2 : Rat brain lysate
    Lane 3 : Mouse brain lysate
    Lane 4 : Cow cerebellum lysate
    Lane 5 : Pig hippocampus lysate

    Predicted band size: 39 kDa

  • Immunofluorescence analysis of rat cerebellum tissue (A) or E20 rat cortical culture (B) stained for Aldolase C using ab190368 at a 1/1000 dilution (green)). Costained with rabbit polyclonal to CFAP (red) at a 1/5000 dilution (A) or with chicken polyclonal antibody to MAP2 (red) at a 1/10000 dilution (B). DAPI was used to staing nuclear DNA (blue).

References

ab190368 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
Yes - washes with TBS-trition 0.025%, 0.1% tween in antibody solution
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde

Ms. Joanne Falck

Verified customer

Submitted Nov 09 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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