Product nameAnti-Aldolase C antibody - Astrocyte Marker
See all Aldolase C primary antibodies
DescriptionRabbit polyclonal to Aldolase C - Astrocyte Marker
Tested applicationsSuitable for: ICC/IF, IHC-Fr, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow, Chimpanzee, Monkey
Synthetic peptide corresponding to Mouse Aldolase C aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- Recombinant Human Aldolase C protein (ab124990) can be used as a positive control in WB. This antibody gave a positive signal in Jurkat whole cell lysate as well as the following tissue lysates: Mouse brain; Mouse skeletal muscle; Rat cerebellum. ICC/IF: PC12 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab87122 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 10 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 39 kDa).|
PathwayCarbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.
Sequence similaritiesBelongs to the class I fructose-bisphosphate aldolase family.
- Information by UniProt
- ALDC antibody
- ALDO C antibody
- aldoc antibody
All lanes : Anti-Aldolase C antibody - Astrocyte Marker (ab87122) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Skeletal Muscle (Mouse) Tissue Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 4 : Cerebellum Rat Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 44 kDa why is the actual band size different from the predicted?
Additional bands at: 55 kDa, 71 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes
The 44 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to Mouse Fructose-bisphosphate aldolase C.
ICC/IF image of ab87122 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab87122, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) PC12 cells at 10µg/ml.
IHC-Fr image of Aldolase C staining on Mouse cortex sections using ab87122 (1:1000). The sections were fixed in paraformaldehyde and permeabilized using 0.4% triton x. The sections were blcoked using 50% serum for 30 mins at 24°C.
This product has been referenced in:
- Xiao ZD et al. Energy stress-induced lncRNA FILNC1 represses c-Myc-mediated energy metabolism and inhibits renal tumor development. Nat Commun 8:783 (2017). Read more (PubMed: 28978906) »
- Li J et al. EZH2-mediated H3K27 trimethylation mediates neurodegeneration in ataxia-telangiectasia. Nat Neurosci 16:1745-53 (2013). Read more (PubMed: 24162653) »