Overview

  • Product name

    Anti-Aldose reductase antibody
    See all Aldose reductase primary antibodies
  • Description

    Rabbit polyclonal to Aldose reductase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat, Rabbit, Cow
  • Immunogen

    Recombinant fragment corresponding to Human Aldose reductase aa 1-316.

  • Positive control

    • 293T, A431, HeLa, HepG2, MOLT4 and Raji whole cell lysates; HeLa cells; Human colon carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab153897 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 36 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies.
  • Tissue specificity

    Highly expressed in embryonic epithelial cells (EUE) in response to osmotic stress.
  • Sequence similarities

    Belongs to the aldo/keto reductase family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • ADR antibody
    • AKR1B 1 antibody
    • Akr1b1 antibody
    • Aldehyde reductase 1 antibody
    • Aldehyde reductase antibody
    • Aldo keto reductase family 1, member B1 antibody
    • Aldo-keto reductase family 1 member B1 antibody
    • aldo-keto reductase family 1, member B1 (aldose reductase) antibody
    • Aldose reductase antibody
    • aldr 1 antibody
    • ALDR_HUMAN antibody
    • aldr1 antibody
    • ALR2 antibody
    • AR antibody
    • Lii5 2 CTCL tumor antigen antibody
    • Low Km aldose reductase antibody
    • MGC1804 antibody
    see all

Images

  • All lanes : Anti-Aldose reductase antibody (ab153897) at 1/2500 dilution

    Lane 1 : WT HeLa cell extract
    Lane 2 : Aldose reductase Knockout HeLa cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 36 kDa

  • All lanes : Anti-Aldose reductase antibody (ab153897) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 36 kDa



    10% SDS PAGE
  • Immunofluorescent analysis of methanol-fixed HeLa cells labeling Aldose reductase  with ab153897 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.

  • Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling Aldose reductase with ab153897 at 1/500 dilution.

References

This product has been referenced in:

  • Chen X  et al. AKR1B1 Upregulation Contributes to Neuroinflammation and Astrocytes Proliferation by Regulating the Energy Metabolism in Rat Spinal Cord Injury. Neurochem Res 43:1491-1499 (2018). Read more (PubMed: 29948725) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Tissue lysate - whole (Prostate)
Gel Running Conditions
Reduced Denaturing
Loading amount
60 µg
Specification
Prostate
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Nov 09 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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