Anti-Aldose reductase antibody (ab153897)

Overview

  • Product name
    Anti-Aldose reductase antibody
    See all Aldose reductase primary antibodies
  • Description
    Rabbit polyclonal to Aldose reductase
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Rabbit, Cow
  • Immunogen

    Recombinant fragment corresponding to Human Aldose reductase aa 1-316.

  • Positive control
    • 293T, A431, HeLa, HepG2, MOLT4 and Raji whole cell lysates; HeLa cells; Human colon carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab153897 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 36 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies.
  • Tissue specificity
    Highly expressed in embryonic epithelial cells (EUE) in response to osmotic stress.
  • Sequence similarities
    Belongs to the aldo/keto reductase family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADR antibody
    • AKR1B 1 antibody
    • Akr1b1 antibody
    • Aldehyde reductase 1 antibody
    • Aldehyde reductase antibody
    • Aldo keto reductase family 1, member B1 antibody
    • Aldo-keto reductase family 1 member B1 antibody
    • aldo-keto reductase family 1, member B1 (aldose reductase) antibody
    • Aldose reductase antibody
    • aldr 1 antibody
    • ALDR_HUMAN antibody
    • aldr1 antibody
    • ALR2 antibody
    • AR antibody
    • Lii5 2 CTCL tumor antigen antibody
    • Low Km aldose reductase antibody
    • MGC1804 antibody
    see all

Images

  • All lanes : Anti-Aldose reductase antibody (ab153897) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 36 kDa



    10% SDS PAGE
  • Immunofluorescent analysis of methanol-fixed HeLa cells labeling Aldose reductase  with ab153897 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.

  • Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling Aldose reductase with ab153897 at 1/500 dilution.

References

ab153897 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Tissue lysate - whole (Prostate)
Gel Running Conditions
Reduced Denaturing
Loading amount
60 µg
Specification
Prostate
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 21°C
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Verified customer

Submitted Nov 09 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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