Anti-Aldose reductase antibody (ab62795)

Reviews (2)Q&A (2)References (2)
Western blot - Anti-Aldose reductase antibody (ab62795)
  • Immunocytochemistry/ Immunofluorescence - Anti-Aldose reductase antibody (ab62795)

Key features and details

  • Rabbit polyclonal to Aldose reductase
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Aldose reductase antibody
    See all Aldose reductase primary antibodies

  • Description

    Rabbit polyclonal to Aldose reductase

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Orangutan

  • Immunogen

    Synthetic peptide corresponding to Human Aldose reductase aa 300 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab92665)

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab62795 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function

    Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies.

  • Tissue specificity

    Highly expressed in embryonic epithelial cells (EUE) in response to osmotic stress.

  • Sequence similarities

    Belongs to the aldo/keto reductase family.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    • Alternative names

      • ADR antibody
      • AKR1B 1 antibody
      • Akr1b1 antibody
      • Aldehyde reductase 1 antibody
      • Aldehyde reductase antibody
      • Aldo keto reductase family 1, member B1 antibody
      • Aldo-keto reductase family 1 member B1 antibody
      • aldo-keto reductase family 1, member B1 (aldose reductase) antibody
      • Aldose reductase antibody
      • aldr 1 antibody
      • ALDR_HUMAN antibody
      • aldr1 antibody
      • ALR2 antibody
      • AR antibody
      • Lii5 2 CTCL tumor antigen antibody
      • Low Km aldose reductase antibody
      • MGC1804 antibody
      see all

    Images

    • Western blot - Anti-Aldose reductase antibody (ab62795)
      Western blot - Anti-Aldose reductase antibody (ab62795)
      All lanes : Anti-Aldose reductase antibody (ab62795) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
      Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
      Lane 4 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
      Lane 5 : Human kidney tissue lysate - total protein (ab30203)
      Lane 6 : Human placenta tissue lysate - total protein (ab29745)
      Lane 7 : MEL-1 (Human embryonic stem cell, male cell line) Whole Cell Lysate (ab27198)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 36 kDa
      Observed band size: 36 kDa
      Additional bands at: 44 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 3 minutes
    • Immunocytochemistry/ Immunofluorescence - Anti-Aldose reductase antibody (ab62795)
      Immunocytochemistry/ Immunofluorescence - Anti-Aldose reductase antibody (ab62795)

      ICC/IF image of Aldose reductase stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab62795 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    References (2)

    Publishing research using ab62795? Please let us know so that we can cite the reference in this datasheet.

    ab62795 has been referenced in 2 publications.

    • Ramos RJ  et al. Discovery of pyridoxal reductase activity as part of human vitamin B6 metabolism. Biochim Biophys Acta Gen Subj 1863:1088-1097 (2019). PubMed: 30928491
    • Hevir N  et al. Decreased levels of AKR1B1 and AKR1B10 in cancerous endometrium compared to adjacent non-cancerous tissue. Chem Biol Interact : (2012). IHC-P, WB ; Human . PubMed: 23146748

    Customer reviews and Q&As

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    1-4 of 4 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Aldose reductase antibody

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (endometrial cancer)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: sodium citrate buffer (pH=6) in microwave oven
    Permeabilization
    No
    Specification
    endometrial cancer
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 13 2013

    Western blot abreview for Anti-Aldose reductase antibody

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (endometrial cancer and adjacent control endometriu)
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Loading amount
    30 µg
    Specification
    endometrial cancer and adjacent control endometriu
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 12 2013

    Question

    Inquiry: Hello, I am writing in regards to the specificity of the anti-AKR1B1 primary antibody for the AKR1B1 isoform compared to the AKR1B10 isoform for use in Western blotting. Recombinant human AKR1B10 protein (50, 25, 10, or 1 ng) or 5 ug A549 whole cell lysate was separated on a 10% SDS gel under reducing conditions (samples loaded on gel in duplicate) at 90 V in SDS running buffer at RT. Protein transferred to PVDF membrane overnight at 30 V at 4C in Tris-Glycine transfer buffer with 10% MeOH Membrane blocked in 5% milk in TBST for 1 hr at RT Membrane cut into two pieces with same samples on each half so samples could be probed independently with two different antibodies (no stripping). Membranes incubated in either 1 ug/mL anti-AKR1B1 or 1:2000 anti-AKR1B10 primary antibody 1 hr at RT. Membranes washed with TBST 3 times, 10 min each wash. Membranes incubated with 1:5000 donkey anti-rabbit HRP secondary antibody 1 hr at RT. Membranes washed with TBST 3 times, 10 min each wash. Detected protein with Western Lightning chemiluminescence reagent and visualized blots on film with 20 second exposure. My results (image attached) demonstrate that the AKR1B1 antibody is able to detect AKR1B10 protein (36 kDa), indicating that it is not specific for AKR1B1 detection by Western blot. As my work requires me to be able to differentiate between protein levels of 1B1 and 1B10 in cell lysates, the 1B1 antibody that I purchased is not capable of doing this. Although there are other 1B1 antibodies available, I do not feel that these other 1B1 antibodies would be better able to differentiate 1B1 from 1B10 since none of them were made against a full length 1B1 protein sequence. Therefore I am asking for a refund for the AKR1B1 antibody purchased. Thank you for your time and consideration in this matter. I look forward to your response.

    Read More
    Answer

    Thank you for contacting us.

    Your credit note ID is XXXXX


    I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used.

    Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

    The credit note ID is for your reference only and does not automatically guarantee the credit.

    I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

    Read More

    Question

    Dear all,

    I want to purchase AKR1B1 and AKR1B10 antibodies. I have allready selected them on your webside, but then I blasted both proteins and I found thet they have 70.6% identity and 91.1% similarity. Do you think they are similar enough for the crossreactivity of the antibodies? Have you tested them with recombinant proteins to prove that AKR1B1 antibodies do not detect AKR1B10 protein and vice versa?
    I wanted to select antibodies: https://www.abcam.com/Aldose-reductase-antibody-ab62795.html and https://www.abcam.com/AKR1B10-antibody-ab96417.html. Would it be better to select monoclonal antibodies?

    I will wait for your responce before I place my order.

    Regards,

    Read More
    Answer

    Thank you for your inquiry.
    I was in contact with the lab and unfortunately, we did not experimentally test if there is a cross reaction of the antibody to the other proteins.

    In general, we do not expect any cross reactivity if the homology (identity)is below 85%.

    Since the immunogen of both antibodies are short peptide, monoclonal antibodies will not be advantageous in this case.

    I am sorryI could not be of more help and hope this information is nevertheless helpful.Please do not hesitate to contact me again with any further questions.

    Read More

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