Product nameAnti-Aldose reductase antibody
See all Aldose reductase primary antibodies
DescriptionRabbit polyclonal to Aldose reductase
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Orangutan
Synthetic peptide corresponding to Human Aldose reductase aa 300 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available as
- WB: Recombinant Human Aldose reductase protein (ab81765), Human kidney and placenta tissue lysates and HeLa, Jurkat, HEK293, Caco2 and MEL-1 whole cell lysates. ICC/IF formaldehyde fixed HepG2 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab62795 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).|
|IHC-P||Use at an assay dependent concentration. PubMed: 23146748|
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionCatalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies.
Tissue specificityHighly expressed in embryonic epithelial cells (EUE) in response to osmotic stress.
Sequence similaritiesBelongs to the aldo/keto reductase family.
- Information by UniProt
- ADR antibody
- AKR1B 1 antibody
- Akr1b1 antibody
All lanes : Anti-Aldose reductase antibody (ab62795) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lane 5 : Human kidney tissue lysate - total protein (ab30203)
Lane 6 : Human placenta tissue lysate - total protein (ab29745)
Lane 7 : MEL-1 (Human embryonic stem cell, male cell line) Whole Cell Lysate (ab27198)
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 36 kDa
Additional bands at: 44 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
ab62795 staining Aldose reductase in Human endometrial cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% serum for 60 minutes at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100 in 5% Gaot serum in TPBS) for 20 hours at 4°C. A Goat anti-rabbit IgG+IgM H+L polyclonal (1/100) was used as the secondary antibody.
ICC/IF image of Aldose reductase stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab62795 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- Ramos RJ et al. Discovery of pyridoxal reductase activity as part of human vitamin B6 metabolism. Biochim Biophys Acta Gen Subj 1863:1088-1097 (2019). Read more (PubMed: 30928491) »
- Hevir N et al. Decreased levels of AKR1B1 and AKR1B10 in cancerous endometrium compared to adjacent non-cancerous tissue. Chem Biol Interact : (2012). IHC-P, WB ; Human . Read more (PubMed: 23146748) »