Anti-Aldose reductase antibody [CPTC-AKR1B1-2] (ab268058)
Key features and details
- Mouse monoclonal [CPTC-AKR1B1-2] to Aldose reductase
- Suitable for: WB, IHC-P, Protein Array, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Aldose reductase antibody [CPTC-AKR1B1-2]
See all Aldose reductase primary antibodies -
Description
Mouse monoclonal [CPTC-AKR1B1-2] to Aldose reductase -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, Protein Array, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human Aldose reductase aa 1-316.
Database link: P15121 -
Positive control
- WB: Hela cell lysate. ICH/ IF: A549 cells. IHC: Human prostate tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from Bioreactor concentrate -
Clonality
Monoclonal -
Clone number
CPTC-AKR1B1-2 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab268058 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 35 kDa.
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IHC-P |
Use a concentration of 1 - 2 µg/ml.
10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes. |
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Protein Array |
Use at an assay dependent concentration.
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ICC/IF |
Use a concentration of 1 - 2 µg/ml.
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Notes |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 35 kDa. |
IHC-P
Use a concentration of 1 - 2 µg/ml. 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes. |
Protein Array
Use at an assay dependent concentration. |
ICC/IF
Use a concentration of 1 - 2 µg/ml. |
Target
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Function
Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies. -
Tissue specificity
Highly expressed in embryonic epithelial cells (EUE) in response to osmotic stress. -
Sequence similarities
Belongs to the aldo/keto reductase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 231 Human
- Omim: 103880 Human
- SwissProt: P15121 Human
- Unigene: 521212 Human
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Alternative names
- ADR antibody
- AKR1B 1 antibody
- Akr1b1 antibody
see all
Images
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Formalin-fixed, paraffin-embedded human Prostate stained for Aldose reductase using ab268058 at 2 μg/ml in immunohistochemical analysis.
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Anti-Aldose reductase antibody [CPTC-AKR1B1-2] (ab268058) at 2 µg/ml + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Predicted band size: 35 kDa -
Immunofluorescence staining of A549 cells labeling Aldose reductase with ab268058 at 2 ug/ml followed by a goat anti-Mouse IgG-CF488 (Green). Nuclei are labeled with Reddot (Blue)
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Protein Array containing more than 19,000 full-length human proteins using ab268058. Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab268058 has been referenced in 1 publication.
- Shi W et al. Novel role for epalrestat: protecting against NLRP3 inflammasome-driven NASH by targeting aldose reductase. J Transl Med 21:700 (2023). PubMed: 37805545