Recombinant Anti-Aldosterone antibody [EPPTX-R9-134-1] (ab241065)
![ELISA - Anti-Aldosterone antibody [EPPTX-R9-134-1] (ab241065)](https://www.abcam.com/ps/products/241/ab241065/Images/ab241065-333292-anti-aldosterone-antibody-epptx-r9-134-1-elisa.jpg "ELISA - Anti-Aldosterone antibody [EPPTX-R9-134-1] (ab241065)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPPTX-R9-134-1] to Aldosterone
- Suitable for: Competitive ELISA, ELISA
Overview
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Product name
Anti-Aldosterone antibody [EPPTX-R9-134-1]
See all Aldosterone primary antibodies -
Description
Rabbit monoclonal [EPPTX-R9-134-1] to Aldosterone -
Host species
Rabbit -
Tested applications
Suitable for: Competitive ELISA, ELISAmore details -
Immunogen
Chemical/ Small Molecule corresponding to Aldosterone. (Aldosterone-3-CMO-BSA).
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPPTX-R9-134-1 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
Applications
Our Abpromise guarantee covers the use of ab241065 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Competitive ELISA | Use at an assay dependent concentration. Use 20-25ng/ml. |
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| ELISA | Use at an assay dependent concentration. |
Target
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Relevance
Aldosterone is a steroid hormone (mineralocorticoid family) produced by the outer-section (zona glomerulosa) of the adrenal cortex in the adrenal gland to regulate sodium and potassium balance in the blood. It is synthesized from cholesterol by aldosterone synthase, which is absent in other sections of the adrenal gland. It acts in the kidney promoting the reabsorption of sodium ions (Na+) into the blood. Water follows the salt and this helps maintain normal blood pressure. It also acts on sweat glands to reduce the loss of sodium in perspiration and taste cells to increase the sensitivity of the taste buds to sources of sodium. -
Cellular localization
Secreted
Images
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ELISA - Anti-Aldosterone antibody [EPPTX-R9-134-1] (ab241065)
Goat anti-rabbit IgG was coated onto 96-wells. Serial dilution of Aldosterone (0, 25, 50, 100, 200, 400, 800, 1600pg/mL) was added (25μL). This was followed by adding 50μL HRP-conjugated Aldosterone and 20-25ng/mL of ab241065 (50μL) into each well. Plates were incubated for 2 hours at room temperature by shaking. After washing, 125μL of Tetramethylbenzidine (TMB) substrate was added and incubated for 30 minutes without shaking for color development. Then 125μL of stopping solution (acid solution) was dispensed and OD was read at 450 nm on a microplate reader within 5 minutes.
This data was kindly provided by Pantex Division of Bio-Analysis, Inc.
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![Competitive ELISA - Anti-Aldosterone antibody [EPPTX-R9-134-1] (ab241065)](https://www.abcam.com/ps/products/241/ab241065/Images/ab241065-333293-anti-aldosterone-antibody-epptx-r9-134-1-elisa.jpg)
Competitive ELISA - Anti-Aldosterone antibody [EPPTX-R9-134-1] (ab241065)ELISA - Anti-Aldosterone antibody [EPPTX-R9-134-1] (ab241065) at 20 ng/ml.
ab241065 is specific to Aldosterone and the percent cross reactivity with other compounds is demonstrated in the table.
This data was kindly provided by Pantex Division of Bio-Analysis, Inc.
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![Anti-Aldosterone antibody [EPPTX-R9-134-1] (ab241065)](https://www.abcam.com/ps/products/241/ab241065/Images/ab241065-3-benefits-of-recombinant-antibodies.png)
Anti-Aldosterone antibody [EPPTX-R9-134-1] (ab241065)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab241065 has not yet been referenced specifically in any publications.
