Overview

  • Product name
    Aldosterone ELISA kit
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Aldosterone 20 84.8pg/ml 4.5%
    Aldosterone 20 27.7pg/ml 4.4%
    Aldosterone 20 13.9pg/ml 6.6%
    Inter-assay
    Sample n Mean SD CV%
    Aldosterone 13 72.6pg/ml 10.8%
    Aldosterone 13 24.4pg/ml 18%
    Aldosterone 13 10.8pg/ml 16.3%
  • Sample type
    Urine, Serum, Plasma
  • Assay type
    Competitive
  • Sensitivity
    = 4.7 pg/ml
  • Range
    3.9 pg/ml - 250 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Urine = 98.7 7.5pg/ml - 120pg/ml
    Serum = 69.8 7.5pg/ml - 120pg/ml
    Plasma = 108.3 7.5pg/ml - 120pg/ml

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Mammals
  • Product overview

    Abcam’s Aldosterone in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Aldosterone in plasma, serum and urine.

    A donkey anti-sheep IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated- Aldosterone antigen and a polyclonal sheep antibody specific to Aldosterone. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of Aldosterone captured in the plate.

  • Notes
    Compound% Cross Reactivity

    11-Deoxycorticosterone

    0.30%

    Progesterone

    0.20%

    Corticosterone

    0.19%

    Cortisol

    ≤ 0.001%

    DHT

    ≤ 0.001%

    Estradiol

    ≤ 0.001%

    Testosterone

    ≤ 0.001%

  • Tested applications
    Suitable for: Competitive ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab136933 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Images

  • Aldosterone measured in various samples showing quantity (pg) per mL of sample tested.

  • Representative standard curve using ab136933.

Protocols

References

This product has been referenced in:
See all 9 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

human cell culture supernatant

Excellent Excellent 5/5 (Ease of Use)
Abreviews
I've used this assay kit to measure aldosterone production in cell culture supernatant of a H295R human adrenal gland carcinoma cell line. This kit gives reliable values for serial dilutions of samples. The performance of the assay was not influenced by the use of medium instead of assay buffer with a minimum dilution of 1:8.
This is a reliable product to measure aldosteron values in cell culture supernatants.

Miss. Saskia Scheij

Verified customer

Submitted Oct 31 2013

Answer

Hello! Please find the reply from the lab below:

The TA (Total Activity) well is a control that is included in our competitive assays to indicate whether or not the conjugate is working as expected. If the TA value, for example, was normal but the rest of the OD’s were suppressed, then you would be able to have confidence that the suppressed OD’s is not likely caused by an issue with the conjugate; if the TA value was low or at background then you would know that the conjugate may be near expiration, may not have been stored appropriately, may have been accidentally cross contaminated with substrate, has not been stored correctly, etc. The TA well serves only as an “OD check” and is not used to calculate data in any way.

I hope this is useful to you, please let me know if you have any further questions!

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Answer


Unfortunately, we have only validated this assay for plasma, serum and urine. We therefore do not know the best way of preparing the samples nor do we have any tissue related citations for this assay.

Aldosterone itself is a species independent molecule; therefore, assuming that there is enough for the assay to detect and you can demonstrate dilutional linearity, we do not have any reason to suspect that the assay would not be amendable for mouse tissue samples. I would suggest that you review any general literature regarding expected mouse aldosterone levels so that you get an idea for expected baseline level to see if the kit would be compatible.

However, if you would like to test this kit on mouse tissues, I could give you a testing discount.

Simply follow these easy steps below to apply for our AbTrial Program:

1. Reply to this email, letting us know you are interested in testing this product.

2. Our scientists will email you an inactive personal discount code for the value of the product.

3. Purchase and test the product at the regular price.

4. Submit your results, including your discount code in the additional notes section of your Abreview.

5. Once the Abreview is submitted, the discount code will become active.

6. Apply your discount code on your next order to receive that value off.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: https://www.abcam.com/abtrial

I could also suggest the following sample preparation which we recommend when using our other kits:

Cell or tissue lysates for use Antibody Arrays and ELISA kits can be prepared using most conventional methods, e.g. homogenization of cell or tissue in Lysis Buffer. You may also use your own lysis buffer, such as RIPA or other formulations optimized for immunoprecipitation.

Please note the following guidelines on lysis buffer composition:

1) Avoid using >0.1% SDS or other strongly denaturing detergents. In general, non-ionic detergents such as Triton X-100 or NP-40 are best, although zwitterionic detergents such as CHAPS, or mild ionic detergents such as sodium deoxycholate will work.

2) Use no more than 2% v/v total detergent

3) Avoid the use of sodium azide

4) Avoid using >10 mM reducing agents, such as dithiothreitol or mercaptoethanols

We strongly recommend adding a protease inhibitor cocktail to the lysis buffer prior to homogenization. Most general biochemical supply companies including Roche, Sigma-Aldrich, Pierce, and Calbiochem stock a wide variety of these products. Since susceptibility to proteolytic cleavage and the type of proteases present in the lysate vary, we do not recommend a specific product. Instead, your choice of which combination of protease inhibitors to use should be based upon a literature search for your protein(s) of interest and/or tissue or cell type. Phosphatase inhibitors may be used but are not necessary unless the antibodies used in the kit specifically recognize phosphorylated forms of the protein. Choices of the method for lysis and homogenization include glass-bead “smash,” douncing, freezethaw, sonication and crushing frozen tissue with a mortar and pestle, or even a combination of these.

There is no best method for all sample types; your choice of method should be made following a brief search of the literature to see how samples similar to yours have been prepared in previous investigations.

After homogenization, centrifuge the lysates to remove cell/tissue debris (5 min @ 10,000 x g or 10 min @ 5,000 x g) and save the supernatant. Unless testing fresh, lysates should be frozen as soon as possible and stored at -20°C (or -80°C, if possible). Centrifuge them again before incubating with any immunoassay.

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Answer

Thank you very much for your inquiry and your interest in our AbTrial programme.


I am very pleased to hear you would like to test ab138875 with mouse serum, mouse urine and tissue culture supernatant. This provided code will give you £180.00 off your next order before the expiration date. To redeem this offer, please submit an Abreview for mouse serum, mouse urine and tissue culture supernatant tested with this kit and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


DISCOUNT CODE: xxxxxx
Expiration date: 21/03/2013
Value: £180.00


It would be beneficial if a positive control with human cell lysate could be performed to interpret the results.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.


The Aldosterone ELISA kit (ab136933) has already been tested for mouse serum and urine and is guaranteed for this application. Could you confirm what cells you are using for the tissue culture supernatant sample? Are the used cells known to produce Aldosterone? If so we would be pleased to offer you the Abtrial for this kit with a cell culture supernatant sample as well.

I hope this information is helpful for you. Please do not hesitate to come back to me with the requested information and let me know how you would like to proceed.

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