Overview

  • Product name
    Aldosterone ELISA kit
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Aldosterone 20 84.8pg/ml 4.5%
    Aldosterone 20 27.7pg/ml 4.4%
    Aldosterone 20 13.9pg/ml 6.6%
    Inter-assay
    Sample n Mean SD CV%
    Aldosterone 13 72.6pg/ml 10.8%
    Aldosterone 13 24.4pg/ml 18%
    Aldosterone 13 10.8pg/ml 16.3%
  • Sample type
    Urine, Serum, Plasma
  • Assay type
    Competitive
  • Sensitivity
    = 4.7 pg/ml
  • Range
    3.9 pg/ml - 250 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Urine = 98.7 7.5pg/ml - 120pg/ml
    Serum = 69.8 7.5pg/ml - 120pg/ml
    Plasma = 108.3 7.5pg/ml - 120pg/ml

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Mammals
  • Product overview

    Abcam’s Aldosterone in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Aldosterone in plasma, serum and urine.

    A donkey anti-sheep IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated- Aldosterone antigen and a polyclonal sheep antibody specific to Aldosterone. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of Aldosterone captured in the plate.

  • Notes
    Compound% Cross Reactivity

    11-Deoxycorticosterone

    0.30%

    Progesterone

    0.20%

    Corticosterone

    0.19%

    Cortisol

    ≤ 0.001%

    DHT

    ≤ 0.001%

    Estradiol

    ≤ 0.001%

    Testosterone

    ≤ 0.001%

  • Tested applications
    Suitable for: Competitive ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab136933 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Images

  • Aldosterone measured in various samples showing quantity (pg) per mL of sample tested.

  • Representative standard curve using ab136933.

Protocols

References

This product has been referenced in:
See all 9 Publications for this product

Customer reviews and Q&As

Filter by Ratings

human cell culture supernatant

Excellent Excellent 5/5 (Ease of Use)
Abreviews
I've used this assay kit to measure aldosterone production in cell culture supernatant of a H295R human adrenal gland carcinoma cell line. This kit gives reliable values for serial dilutions of samples. The performance of the assay was not influenced by the use of medium instead of assay buffer with a minimum dilution of 1:8.
This is a reliable product to measure aldosteron values in cell culture supernatants.

Miss. Saskia Scheij

Verified customer

Submitted Oct 31 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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