Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 405 Rabbit monoclonal [EPR17526] to ERK1 + ERK2
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: Alexa Fluor® 405. Ex: 402nm, Em: 421nm
Product nameAlexa Fluor® 405 Anti-ERK1 + ERK2 antibody [EPR17526]
See all ERK1 + ERK2 primary antibodies
DescriptionAlexa Fluor® 405 Rabbit monoclonal [EPR17526] to ERK1 + ERK2
ConjugationAlexa Fluor® 405. Ex: 402nm, Em: 421nm
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Recombinant fragment within Human ERK1 + ERK2 aa 150 to the C-terminus. The exact sequence is proprietary.
Database link: P28482
- Flow Cyt: A431 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 30% Glycerol, PBS
Concentration information loading...
PurityProtein A purified
- Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)
- Alexa Fluor® 488 Anti-ERK1 + ERK2 antibody [EPR17526] (ab208564)
- Alexa Fluor® 647 Anti-ERK1 + ERK2 antibody [EPR17526] (ab208881)
- HRP Anti-ERK1 + ERK2 antibody [EPR17526] (ab209321)
- Alexa Fluor® 594 Anti-ERK1 + ERK2 antibody [EPR17526] (ab210460)
- Alexa Fluor® 555 Anti-ERK1 + ERK2 antibody [EPR17526] (ab210659)
- PE Anti-ERK1 + ERK2 antibody [EPR17526] (ab212153)
- Anti-ERK1 + ERK2 antibody [EPR17526] - BSA and Azide free (ab218017)
Our Abpromise guarantee covers the use of ab210044 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FunctionInvolved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain.
DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
modificationsDually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.
- Information by UniProt
- ERK 1 antibody
- ERK 2 antibody
- ERK-1 antibody
Overlay histogram showing A431 cells stained with ab210044 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min, blocked with Image-iT® FX Signal Enhancer for 30 min at 22ºC and then blocked with 1x PBS / 10% normal goat serum for 1 hr at 22ºC. Cells were then incubated with the antibody (ab210044, 1/500 dilution) for 30 min at 22ºC.
Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 405 (ab208150) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50mW violet laser (405nm) and 450/50 bandpass filter.
ab210044 has not yet been referenced specifically in any publications.