Recombinant Alexa Fluor® 405 Anti-ERK1 + ERK2 antibody [EPR17526] (ab210044)
![Flow Cytometry - Alexa Fluor® 405 Anti-ERK1 + ERK2 antibody [EPR17526] (ab210044)](https://www.abcam.com/ps/products/210/ab210044/Images/ab210044-259600-ab210044FLOW1.jpg "Flow Cytometry - Alexa Fluor® 405 Anti-ERK1 + ERK2 antibody [EPR17526] (ab210044)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 405 Rabbit monoclonal [EPR17526] to ERK1 + ERK2
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: Alexa Fluor® 405. Ex: 402nm, Em: 421nm
Overview
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Product name
Alexa Fluor® 405 Anti-ERK1 + ERK2 antibody [EPR17526]
See all ERK1 + ERK2 primary antibodies -
Description
Alexa Fluor® 405 Rabbit monoclonal [EPR17526] to ERK1 + ERK2 -
Host species
Rabbit -
Conjugation
Alexa Fluor® 405. Ex: 402nm, Em: 421nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant fragment within Human ERK1 + ERK2 aa 150 to the C-terminus. The exact sequence is proprietary.
Database link: P28482 -
Positive control
- Flow Cyt: A431 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 30% Glycerol, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17526 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)
- Alexa Fluor® 488 Anti-ERK1 + ERK2 antibody [EPR17526] (ab208564)
- Alexa Fluor® 647 Anti-ERK1 + ERK2 antibody [EPR17526] (ab208881)
- HRP Anti-ERK1 + ERK2 antibody [EPR17526] (ab209321)
- Alexa Fluor® 594 Anti-ERK1 + ERK2 antibody [EPR17526] (ab210460)
- Alexa Fluor® 555 Anti-ERK1 + ERK2 antibody [EPR17526] (ab210659)
- PE Anti-ERK1 + ERK2 antibody [EPR17526] (ab212153)
- Anti-ERK1 + ERK2 antibody [EPR17526] - BSA and Azide free (ab218017)
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab210044 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/500. |
Target
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Function
Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
Domain
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. -
Post-translational
modificationsDually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5594 Human
- Entrez Gene: 5595 Human
- Entrez Gene: 26413 Mouse
- Entrez Gene: 26417 Mouse
- Entrez Gene: 116590 Rat
- Entrez Gene: 50689 Rat
- Omim: 176948 Human
- Omim: 601795 Human
see all -
Alternative names
- ERK 1 antibody
- ERK 2 antibody
- ERK-1 antibody
see all
Images
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Flow Cytometry - Alexa Fluor® 405 Anti-ERK1 + ERK2 antibody [EPR17526] (ab210044)
Overlay histogram showing A431 cells stained with ab210044 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min, blocked with Image-iT® FX Signal Enhancer for 30 min at 22ºC and then blocked with 1x PBS / 10% normal goat serum for 1 hr at 22ºC. Cells were then incubated with the antibody (ab210044, 1/500 dilution) for 30 min at 22ºC.
Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 405 (ab208150) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50mW violet laser (405nm) and 450/50 bandpass filter.
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![Alexa Fluor® 405 Anti-ERK1 + ERK2 antibody [EPR17526] (ab210044)](https://www.abcam.com/ps/products/210/ab210044/Images/ab210044-3-benefits-of-recombinant-antibodies.png)
Alexa Fluor® 405 Anti-ERK1 + ERK2 antibody [EPR17526] (ab210044)
Datasheets and documents
Certificate of Compliance
References (0)
ab210044 has not yet been referenced specifically in any publications.
