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RecombinantRabMAb

Recombinant Alexa Fluor® 405 Anti-FAK antibody [EP695Y] (ab210014)

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  • Certificate of Compliance
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Flow Cytometry - Alexa Fluor® 405 Anti-FAK antibody [EP695Y] (ab210014)
  • Alexa Fluor® 405 Anti-FAK antibody [EP695Y] (ab210014)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 405 Rabbit monoclonal [EP695Y] to FAK
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 405. Ex: 402nm, Em: 421nm

You may also be interested in

Protein
Product image
Recombinant human FAK protein (ab105909)
Conjugation
Product image
DyLight® 405 Conjugation Kit (Fast) - Lightning-Link® (ab201798)

View more associated products

Overview

  • Product name

    Alexa Fluor® 405 Anti-FAK antibody [EP695Y]
    See all FAK primary antibodies
  • Description

    Alexa Fluor® 405 Rabbit monoclonal [EP695Y] to FAK
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 405. Ex: 402nm, Em: 421nm
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt: SH-SY5Y cells
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP695Y
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Adhesion / ECM
    • Integrins
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • FAK / PYK
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • Structures
    • Focal Adhesions
    • Cancer
    • Signal transduction
    • Protein phosphorylation
    • Tyrosine kinases
    • FAK family

Associated products

  • Alternative Versions

    • PE Anti-FAK antibody [EP695Y] (ab209436)
    • Anti-FAK antibody [EP695Y] - Low endotoxin, Azide free (ab219363)
    • Anti-FAK antibody [EP695Y] - BSA and Azide free (ab271836)
    • Anti-FAK antibody [EP695Y] (ab40794)
  • Isotype control

    • Alexa Fluor® 405 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208150)
  • Recombinant Protein

    • Recombinant human FAK protein (ab105909)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab210014 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
All applications
Mouse
Rat
Cow
Application Abreviews Notes
Flow Cyt
1/50.

The cellular localisation of this product has been verified in ICC/IF

Notes
Flow Cyt
1/50.

The cellular localisation of this product has been verified in ICC/IF

Target

  • Function

    Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
  • Tissue specificity

    Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
    Contains 1 FERM domain.
    Contains 1 protein kinase domain.
  • Domain

    The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL.
    The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.
  • Post-translational
    modifications

    Phosphorylated on 6 tyrosine residues upon activation. Microtubule-induced dephosphorylation at Tyr-397 could be catalyzed by PTPN11 and regulated by ZFYVE21. Dephosphorylated by PTPN11 upon EPHA2 activation by its ligand EFNA1.
  • Cellular localization

    Cell junction > focal adhesion. Cell membrane. Constituent of focal adhesions.
  • Target information above from: UniProt accession Q05397 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 506270 Cow
    • Entrez Gene: 5747 Human
    • Entrez Gene: 14083 Mouse
    • Entrez Gene: 25614 Rat
    • Omim: 600758 Human
    • SwissProt: Q05397 Human
    • SwissProt: P34152 Mouse
    • SwissProt: O35346 Rat
    • Unigene: 395482 Human
    • Unigene: 254494 Mouse
    • Unigene: 2809 Rat
    see all
  • Alternative names

    • FADK 1 antibody
    • FADK antibody
    • FAK related non kinase polypeptide antibody
    • FAK1 antibody
    • FAK1_HUMAN antibody
    • Focal adhesion kinase 1 antibody
    • Focal adhesion Kinase antibody
    • Focal adhesion kinase isoform FAK Del33 antibody
    • Focal adhesion kinase related nonkinase antibody
    • FRNK antibody
    • p125FAK antibody
    • pp125FAK antibody
    • PPP1R71 antibody
    • Protein phosphatase 1 regulatory subunit 71 antibody
    • Protein tyrosine kinase 2 antibody
    • Protein-tyrosine kinase 2 antibody
    • Ptk2 antibody
    • PTK2 protein tyrosine kinase 2 antibody
    see all

Images

  • Flow Cytometry - Alexa Fluor® 405 Anti-FAK antibody [EP695Y] (ab210014)
    Flow Cytometry - Alexa Fluor® 405 Anti-FAK antibody [EP695Y] (ab210014)

    Overlay histogram showing SH-SY5Y cells stained with ab210014 (red line). The cells were fixed with 80% methanal (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min, blocked with Image-iT® FX Signal Enhancer for 30 min at 22°C and then blocked with 1x PBS / 10% normal goat serum for 30 min at 22°C. Cells were then incubated with the antibody (ab210014, 1/50 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 405 (ab208150) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50mW Violet laser (405nm) and 450/50 bandpass filter.

  • Alexa Fluor® 405 Anti-FAK antibody [EP695Y] (ab210014)
    Alexa Fluor® 405 Anti-FAK antibody [EP695Y] (ab210014)

Protocols

  • Flow cytometry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (0)

Publishing research using ab210014? Please let us know so that we can cite the reference in this datasheet.

ab210014 has not yet been referenced specifically in any publications.

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