Recombinant Alexa Fluor® 405 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab210438)
![Flow Cytometry - Alexa Fluor® 405 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab210438)](https://www.abcam.com/ps/products/210/ab210438/Images/ab210438-259652-anti-glucose-transporter-glut1-antibody-epr3915-alexa-fluor-405-flow-cytometry.jpg "Flow Cytometry - Alexa Fluor® 405 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab210438)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 405 Rabbit monoclonal [EPR3915] to Glucose Transporter GLUT1
- Suitable for: Flow Cyt, WB
- Reacts with: Human
- Conjugation: Alexa Fluor® 405. Ex: 402nm, Em: 421nm
Overview
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Product name
Alexa Fluor® 405 Anti-Glucose Transporter GLUT1 antibody [EPR3915]
See all Glucose Transporter GLUT1 primary antibodies -
Description
Alexa Fluor® 405 Rabbit monoclonal [EPR3915] to Glucose Transporter GLUT1 -
Host species
Rabbit -
Conjugation
Alexa Fluor® 405. Ex: 402nm, Em: 421nm -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Human
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Immunogen
This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab202335) -
Positive control
- Flow Cyt: HepG2 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
EPR3915 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730)
- Alexa Fluor® 647 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab195020)
- HRP Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab195021)
- Alexa Fluor® 488 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab195359)
- Anti-Glucose Transporter GLUT1 antibody [EPR3915] - Low endotoxin, Azide free (ab196357)
- Alexa Fluor® 594 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab206360)
- PE Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab209449)
- Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403)
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Immunizing Peptide (Blocking)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab210438 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Flow Cyt |
Human
|
| All applications |
Mouse
Rat
|
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | (2) |
1/50.
|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 54 kDa.Can be blocked with Glucose Transporter GLUT1 peptide (ab202335).
|
| Notes |
|---|
|
Flow Cyt
1/50. |
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 54 kDa.Can be blocked with Glucose Transporter GLUT1 peptide (ab202335). |
Target
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Function
Facilitative glucose transporter. This isoform may be responsible for constitutive or basal glucose uptake. Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses. -
Tissue specificity
Expressed at variable levels in many human tissues. -
Involvement in disease
Defects in SLC2A1 are the cause of glucose transporter type 1 deficiency syndrome (GLUT1DS) [MIM:606777]; also known as blood-brain barrier glucose transport defect. This disease causes a defect in glucose transport across the blood-brain barrier. It is characterized by infantile seizures, delayed development, and acquired microcephaly.
Defects in SLC2A1 are the cause of dystonia type 18 (DYT18) [MIM:612126]. DYT18 is an exercise-induced paroxysmal dystonia/dyskinesia. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYT18 is characterized by attacks of involuntary movements triggered by certain stimuli such as sudden movement or prolonged exercise. In some patients involuntary exertion-induced dystonic, choreoathetotic, and ballistic movements may be associated with macrocytic hemolytic anemia. -
Sequence similarities
Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cell membrane. Melanosome. Localizes primarily at the cell surface (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 6513 Human
- Entrez Gene: 20525 Mouse
- Entrez Gene: 24778 Rat
- Omim: 138140 Human
- SwissProt: P11166 Human
- SwissProt: P17809 Mouse
- SwissProt: P11167 Rat
- Unigene: 473721 Human
see all -
Alternative names
- Choreoathetosis/spasticity episodic (paroxysmal choreoathetosis/spasticity) antibody
- CSE antibody
- DYT17 antibody
see all
Images
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Flow Cytometry - Alexa Fluor® 405 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab210438)
Overlay histogram showing HepG2 cells stained with ab210438 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min, blocked with Image-iT® FX Signal Enhancer for 30 min at 22ºC and then blocked with 1x PBS / 10% normal goat serum for 1 hr at 22ºC. Cells were then incubated with the antibody (ab210438, 1/50 dilution) for 30 min at 22ºC.
Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 405 (ab208150) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50mW violet laser (405nm) and 450/50 bandpass filter.
This antibody gave a positive signal in HepG2 cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
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![Western blot - Alexa Fluor® 405 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab210438)](https://www.abcam.com/ps/products/210/ab210438/Images/ab210438-365498-rabbit-monoclonal-epr3915-to-glucose-transporter-glut1-western-blot-knockout-og-a549-wt-a549-slc2a1-ko-ht29-hepg2-humanlysates.jpg)
Western blot - Alexa Fluor® 405 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab210438)Clone EPR3915 has been knock-out validated in its unconjugated form. Please refer to ab115730 datasheet for experimental details.
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![Alexa Fluor® 405 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab210438)](https://www.abcam.com/ps/products/210/ab210438/Images/ab210438-4-benefits-of-recombinant-antibodies.png)
Alexa Fluor® 405 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab210438)
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab210438 has not yet been referenced specifically in any publications.