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Signal Transduction Metabolism Plasma Membrane Channels
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RecombinantRabMAb

Recombinant Alexa Fluor® 405 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab210438)

  • Datasheet
  • SDS
  • Certificate of Compliance
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Flow Cytometry - Alexa Fluor® 405 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab210438)
  • Western blot - Alexa Fluor® 405 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab210438)
  • Alexa Fluor® 405 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab210438)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 405 Rabbit monoclonal [EPR3915] to Glucose Transporter GLUT1
  • Suitable for: Flow Cyt, WB
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 405. Ex: 402nm, Em: 421nm

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Overview

  • Product name

    Alexa Fluor® 405 Anti-Glucose Transporter GLUT1 antibody [EPR3915]
    See all Glucose Transporter GLUT1 primary antibodies
  • Description

    Alexa Fluor® 405 Rabbit monoclonal [EPR3915] to Glucose Transporter GLUT1
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 405. Ex: 402nm, Em: 421nm
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    See all applications and species data
  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab202335)

  • Positive control

    • Flow Cyt: HepG2 cells.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR3915
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • Channels
    • Cardiovascular
    • Atherosclerosis
    • Diabetes associated
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Metabolism
    • Types of disease
    • Diabetes
    • Metabolism
    • Types of disease
    • Heart disease

Associated products

  • Alternative Versions

    • Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730)
    • Alexa Fluor® 647 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab195020)
    • HRP Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab195021)
    • Alexa Fluor® 488 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab195359)
    • Anti-Glucose Transporter GLUT1 antibody [EPR3915] - Low endotoxin, Azide free (ab196357)
    • Alexa Fluor® 594 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab206360)
    • PE Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab209449)
    • Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403)
  • Immunizing Peptide (Blocking)

    • Glucose Transporter GLUT1 peptide (ab202335)
  • Isotype control

    • Alexa Fluor® 405 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208150)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab210438 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
All applications
Mouse
Rat
Application Abreviews Notes
Flow Cyt (2)
1/50.
WB
Use at an assay dependent concentration. Predicted molecular weight: 54 kDa.Can be blocked with Glucose Transporter GLUT1 peptide (ab202335).
Notes
Flow Cyt
1/50.
WB
Use at an assay dependent concentration. Predicted molecular weight: 54 kDa.Can be blocked with Glucose Transporter GLUT1 peptide (ab202335).

Target

  • Function

    Facilitative glucose transporter. This isoform may be responsible for constitutive or basal glucose uptake. Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses.
  • Tissue specificity

    Expressed at variable levels in many human tissues.
  • Involvement in disease

    Defects in SLC2A1 are the cause of glucose transporter type 1 deficiency syndrome (GLUT1DS) [MIM:606777]; also known as blood-brain barrier glucose transport defect. This disease causes a defect in glucose transport across the blood-brain barrier. It is characterized by infantile seizures, delayed development, and acquired microcephaly.
    Defects in SLC2A1 are the cause of dystonia type 18 (DYT18) [MIM:612126]. DYT18 is an exercise-induced paroxysmal dystonia/dyskinesia. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYT18 is characterized by attacks of involuntary movements triggered by certain stimuli such as sudden movement or prolonged exercise. In some patients involuntary exertion-induced dystonic, choreoathetotic, and ballistic movements may be associated with macrocytic hemolytic anemia.
  • Sequence similarities

    Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cell membrane. Melanosome. Localizes primarily at the cell surface (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Target information above from: UniProt accession P11166 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6513 Human
    • Entrez Gene: 20525 Mouse
    • Entrez Gene: 24778 Rat
    • Omim: 138140 Human
    • SwissProt: P11166 Human
    • SwissProt: P17809 Mouse
    • SwissProt: P11167 Rat
    • Unigene: 473721 Human
    • Unigene: 721551 Human
    • Unigene: 21002 Mouse
    • Unigene: 3205 Rat
    see all
  • Alternative names

    • Choreoathetosis/spasticity episodic (paroxysmal choreoathetosis/spasticity) antibody
    • CSE antibody
    • DYT17 antibody
    • DYT18 antibody
    • DYT9 antibody
    • EIG12 antibody
    • erythrocyte/brain antibody
    • Erythrocyte/hepatoma glucose transporter antibody
    • facilitated glucose transporter member 1 antibody
    • Glucose transporter 1 antibody
    • Glucose transporter type 1 antibody
    • Glucose transporter type 1, erythrocyte/brain antibody
    • GLUT antibody
    • GLUT-1 antibody
    • GLUT1 antibody
    • GLUT1DS antibody
    • GLUTB antibody
    • GT1 antibody
    • GTG1 antibody
    • Gtg3 antibody
    • GTR1_HUMAN antibody
    • HepG2 glucose transporter antibody
    • HTLVR antibody
    • Human T cell leukemia virus (I and II) receptor antibody
    • MGC141895 antibody
    • MGC141896 antibody
    • PED antibody
    • RATGTG1 antibody
    • Receptor for HTLV 1 and HTLV 2 antibody
    • SLC2A1 antibody
    • Solute carrier family 2 (facilitated glucose transporter), member 1 antibody
    • Solute carrier family 2 antibody
    • Solute carrier family 2, facilitated glucose transporter member 1 antibody
    see all

Images

  • Flow Cytometry - Alexa Fluor® 405 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab210438)
    Flow Cytometry - Alexa Fluor® 405 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab210438)

    Overlay histogram showing HepG2 cells stained with ab210438 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min, blocked with Image-iT® FX Signal Enhancer for 30 min at 22ºC and then blocked with 1x PBS / 10% normal goat serum for 1 hr at 22ºC. Cells were then incubated with the antibody (ab210438, 1/50 dilution) for 30 min at 22ºC.

    Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 405 (ab208150) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50mW violet laser (405nm) and 450/50 bandpass filter.

    This antibody gave a positive signal in HepG2 cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

  • Western blot - Alexa Fluor® 405 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab210438)
    Western blot - Alexa Fluor® 405 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab210438)

    Clone EPR3915 has been knock-out validated in its unconjugated form. Please refer to ab115730 datasheet for experimental details.

  • Alexa Fluor® 405 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab210438)
    Alexa Fluor® 405 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab210438)

Protocols

  • Flow cytometry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (0)

Publishing research using ab210438? Please let us know so that we can cite the reference in this datasheet.

ab210438 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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1-5 of 5 Abreviews or Q&A

Immunohistochemistry free floating abreview for Alexa Fluor® 405 Anti-Glucose Transporter GLUT1 antibody [EPR3915]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry free floating
Sample
Rat Tissue sections (Cerebellum)
Specification
Cerebellum
Read More

Abcam user community

Verified customer

Submitted Feb 23 2021

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Glucose Transporter GLUT1 antibody [EPR3915] (Alexa Fluor® 405)

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Cerebellum)
Antigen retrieval step
None
Permeabilization
Yes - PBS 1x, Triton x-100 1%
Specification
Cerebellum
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Mar 17 2020

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Glucose Transporter GLUT1 antibody [EPR3915] (Alexa Fluor® 405)

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
Yes - PBS 1x, Triton x-100 1%
Specification
Brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Mar 09 2020

Flow Cytometry abreview for Anti-Glucose Transporter GLUT1 antibody [EPR3915] (Alexa Fluor® 405)

Excellent
Abreviews
Abreviews
abreview image
Application
Flow Cytometry
Sample
Rat Cell (Peritoneal lavage cells)
Permeabilization
Yes - 0.1% Triton X-100
Gating Strategy
Viable cells (7AAD) -> Low FSC/SSC (lymphocytes) vs High FSC/SSC (Myeloid)
Specification
Peritoneal lavage cells
Preparation
Cell harvesting/tissue preparation method: Peritoneal lavage with saline
Sample buffer: PBS/2 % FCS
Fixation
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Nov 08 2019

Flow Cytometry abreview for Anti-Glucose Transporter GLUT1 antibody [EPR3915] (Alexa Fluor® 405)

Excellent
Abreviews
Abreviews
abreview image
Application
Flow Cytometry
Sample
Mouse Cell (Liver macrophages)
Permeabilization
Yes - 0.1% Triton X-100
Gating Strategy
Viable cells (7AAD) -> F480+/Cd11b+
Specification
Liver macrophages
Preparation
Cell harvesting/tissue preparation method: Liver tissue was disaggregated in collagenase/DNAse solution and the non-parenchymal cell fraction was isolated using a 30% Percoll gradient.
Sample buffer: PBS/2 % FCS
Fixation
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Nov 08 2019

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