Recombinant Alexa Fluor® 405 Anti-M6PR (cation independent) antibody [EPR6599] - Lysosome Membrane Marker (ab198515)
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 405 Anti-M6PR (cation independent) antibody [EPR6599] - Lysosome Membrane Marker (ab198515)](https://www.abcam.com/ps/products/198/ab198515/Images/ab198515-265774-anti-m6pr-cation-independent-antibody-epr6599-lysosome-membrane-marker-alexa-fluor-405-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 405 Anti-M6PR (cation independent) antibody [EPR6599] - Lysosome Membrane Marker (ab198515)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 405 Rabbit monoclonal [EPR6599] to M6PR (cation independent) - Lysosome Membrane Marker
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 405. Ex: 402nm, Em: 421nm
Related conjugates and formulations
Overview
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Product name
Alexa Fluor® 405 Anti-M6PR (cation independent) antibody [EPR6599] - Lysosome Membrane Marker
See all M6PR (cation independent) primary antibodies -
Description
Alexa Fluor® 405 Rabbit monoclonal [EPR6599] to M6PR (cation independent) - Lysosome Membrane Marker -
Host species
Rabbit -
Conjugation
Alexa Fluor® 405. Ex: 402nm, Em: 421nm -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab169957) -
Positive control
- ICC/IF - CaCo2 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Dissociation constant (KD)
KD = 3.90 x 10 -12 M Learn more about KD -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68.98% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6599 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)
- Alexa Fluor® 488 Anti-M6PR (cation independent) antibody [EPR6599] - Lysosome Membrane Marker (ab184807)
- Alexa Fluor® 647 Anti-M6PR (cation independent) antibody [EPR6599] - Lysosome Membrane Marker (ab198324)
- Alexa Fluor® 594 Anti-M6PR (cation independent) antibody [EPR6599] - Lysosome Membrane Marker (ab203437)
- Alexa Fluor® 555 Anti-M6PR (cation independent) antibody [EPR6599] - Lysosome Membrane Marker (ab203438)
- APC Anti-M6PR (cation independent) antibody [EPR6599] - Lysosome Membrane Marker (ab225131)
- PE Anti-M6PR (cation independent) antibody [EPR6599] (ab225132)
- Anti-M6PR (cation independent) antibody [EPR6599] - BSA and Azide free (ab226090)
- Autophagy Analysis (ATG16L1, ATG16L1 pS278, SQSTM1, LC3B, Ubiquitin, M6PR) Antibody Sampler Panel (ab269811)
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Immunizing Peptide (Blocking)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab198515 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/100.
This product gave a positive signal in CaCo2 cells fixed with 4% Formaldehyde (10 min) and 100% methanol (5 min). |
| Notes |
|---|
|
ICC/IF
1/100. This product gave a positive signal in CaCo2 cells fixed with 4% Formaldehyde (10 min) and 100% methanol (5 min). |
Target
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Function
Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. Acts as a positive regulator of T-cell coactivation, by binding DPP4. -
Sequence similarities
Belongs to the MRL1/IGF2R family.
Contains 1 fibronectin type-II domain. -
Domain
Contains 15 repeating units of approximately 147 AA harboring four disulfide bonds each. The most highly conserved region within the repeat consists of a stretch of 13 AA that contains cysteines at both ends. -
Cellular localization
Lysosome membrane. Colocalized with DPP4 in internalized cytoplasmic vesicles adjacent to the cell surface. - Information by UniProt
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Database links
- Entrez Gene: 3482 Human
- Omim: 147280 Human
- SwissProt: P11717 Human
- Unigene: 487062 Human
- Unigene: 673278 Human
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Alternative names
- 300 kDa mannose 6 phosphate receptor antibody
- 300 kDa mannose 6-phosphate receptor antibody
- Cation independent mannose 6 phosphate receptor antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 405 Anti-M6PR (cation independent) antibody [EPR6599] - Lysosome Membrane Marker (ab198515)
ab198515 staining M6PR in CaCo2 cells. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes, blocked with Image-iT® FX Signal Enhancer at room temperature for 30 min and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198515 at 1/100 dilution (shown in blue). Nuclear DNA was labelled with ab108410, DRAQ5™ at 1.25uM (1/4000 dilution) (shown in red).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in CaCo2 cells fixed with 100% methanol (5 min).
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![Alexa Fluor® 405 Anti-M6PR (cation independent) antibody [EPR6599] - Lysosome Membrane Marker (ab198515)](https://www.abcam.com/ps/products/198/ab198515/Images/ab198515-3-benefits-of-recombinant-antibodies.png)
Alexa Fluor® 405 Anti-M6PR (cation independent) antibody [EPR6599] - Lysosome Membrane Marker (ab198515)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab198515 has not yet been referenced specifically in any publications.