RecombinantRabMAb

Recombinant Alexa Fluor® 405 Anti-NEDD8 antibody [Y297] (ab207248)

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Flow Cytometry (Intracellular) - Alexa Fluor® 405 Anti-NEDD8 antibody [Y297] (ab207248)
  • Alexa Fluor® 405 Anti-NEDD8 antibody [Y297] (ab207248)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 405 Rabbit monoclonal [Y297] to NEDD8
  • Suitable for: Flow Cyt (Intra)
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 405. Ex: 402nm, Em: 421nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free Unconjugated

Overview

  • Product name

    Alexa Fluor® 405 Anti-NEDD8 antibody [Y297]
    See all NEDD8 primary antibodies

  • Description

    Alexa Fluor® 405 Rabbit monoclonal [Y297] to NEDD8

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 405. Ex: 402nm, Em: 421nm

  • Tested applications

    Suitable for: Flow Cyt (Intra)more details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat

  • Immunogen

    Synthetic peptide corresponding to Human NEDD8 (N terminal).
    Database link: Q15843

  • Positive control

    • Flow Cyt (intra)ometry: A673 cells

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    Y297

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab207248 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/50.

The cellular localisation of this product has been verified in ICC/IF
Notes
Flow Cyt (Intra)
1/50.

The cellular localisation of this product has been verified in ICC/IF

Target

  • Function

    Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.

  • Tissue specificity

    Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes.

  • Sequence similarities

    Belongs to the ubiquitin family.

  • Post-translational
    modifications

    Cleavage of precursor form by UCHL3 or SENP8 is necessary for function.

  • Cellular localization

    Nucleus. Mainly nuclear.
  • Information by UniProt

  • Database links

    • Alternative names

      • FLJ43224 antibody
      • MGC104393 antibody
      • MGC125896 antibody
      • MGC125897 antibody
      • NED8 antibody
      • NEDD 8 antibody
      • NEDD-8 antibody
      • Nedd8 antibody
      • NEDD8_HUMAN antibody
      • Neddylin antibody
      • Neural precursor cell expressed developmentally down regulated 8 antibody
      • Neural precursor cell expressed developmentally down regulated gene 8 antibody
      • Neural precursor cell expressed developmentally down-regulated protein 8 antibody
      • Rub1 antibody
      • Ubiquitin like protein Nedd 8 antibody
      • Ubiquitin like protein Nedd8 antibody
      • Ubiquitin-like protein Nedd8 antibody
      see all

    Images

    • Flow Cytometry (Intracellular) - Alexa Fluor® 405 Anti-NEDD8 antibody [Y297] (ab207248)
      Flow Cytometry (Intracellular) - Alexa Fluor® 405 Anti-NEDD8 antibody [Y297] (ab207248)

      Overlay histogram showing A673 cells stained with ab207248 (red line). The cells were fixed with 4% formaldehyde and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab207248, 1/50 dilution) for 30 min at 22°C.

      Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor® 405 (ab208150) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

      Acquisition of >5,000 events were collected using a 50 mW Violet laser (405nm) and 450/50 bandpass filter.

      This antibody gave a positive signal in A673 cells fixed with 80% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

    • Alexa Fluor® 405 Anti-NEDD8 antibody [Y297] (ab207248)
      Alexa Fluor® 405 Anti-NEDD8 antibody [Y297] (ab207248)

    References (0)

    Publishing research using ab207248? Please let us know so that we can cite the reference in this datasheet.

    ab207248 has not yet been referenced specifically in any publications.

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