Recombinant Alexa Fluor® 488 Anti-53BP1 antibody [EPR2172(2)] (ab237174)
![Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-53BP1 antibody [EPR2172(2)] (ab237174)](https://www.abcam.com/ps/products/237/ab237174/Images/ab237174-2-ab237174FLOW1.jpg "Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-53BP1 antibody [EPR2172(2)] (ab237174)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 488 Rabbit monoclonal [EPR2172(2)] to 53BP1
- Suitable for: Flow Cyt (Intra), ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Related conjugates and formulations
Overview
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Product name
Alexa Fluor® 488 Anti-53BP1 antibody [EPR2172(2)]
See all 53BP1 primary antibodies -
Description
Alexa Fluor® 488 Rabbit monoclonal [EPR2172(2)] to 53BP1 -
Host species
Rabbit -
Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
Tested applications
Suitable for: Flow Cyt (Intra), ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: UV treated HeLa cells Flow Cyt (intra): HeLa cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2172(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab237174 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/50.
|
|
| ICC/IF |
1/100.
This product gave a positive signal in UV treated HeLa cells fixed with 4% formaldehyde (10 min) |
| Notes |
|---|
|
Flow Cyt (Intra)
1/50. |
|
ICC/IF
1/100. This product gave a positive signal in UV treated HeLa cells fixed with 4% formaldehyde (10 min) |
Target
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Function
May have a role in checkpoint signaling during mitosis. Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage. -
Involvement in disease
Note=A chromosomal aberration involving TP53BP1 is found in a form of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;15)(q33;q22) with PDGFRB creating a TP53BP1-PDGFRB fusion protein. -
Sequence similarities
Contains 2 BRCT domains. -
Post-translational
modificationsAsymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding.
Phosphorylated at basal level in the absence of DNA damage. Hyper-phosphorylated in an ATM-dependent manner in response to DNA damage induced by ionizing radiation. Hyper-phosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation. -
Cellular localization
Nucleus. Chromosome > centromere > kinetochore. Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. Methylation of histone H4 at 'Lys-20' is required for efficient localization to double strand breaks. - Information by UniProt
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Database links
- Entrez Gene: 7158 Human
- Entrez Gene: 27223 Mouse
- Entrez Gene: 296099 Rat
- Omim: 605230 Human
- SwissProt: Q12888 Human
- SwissProt: P70399 Mouse
- Unigene: 440968 Human
- Unigene: 383499 Mouse
see all -
Alternative names
- 53 BP1 antibody
- 53BP1 antibody
- FLJ41424 antibody
see all
Images
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Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-53BP1 antibody [EPR2172(2)] (ab237174)
Overlay histogram showing HeLa cells stained with ab237174 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab237174, 1/50 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor® 488 (ab199091) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.
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![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-53BP1 antibody [EPR2172(2)] (ab237174)](https://www.abcam.com/ps/products/237/ab237174/Images/ab237174-336158-anti-53bp1-antibody-epr21722-alexa-fluor-488-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-53BP1 antibody [EPR2172(2)] (ab237174)ab237174 staining 53BP1 in UV treated HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab237174 at 1/100 dilution (shown in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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![Alexa Fluor® 488 Anti-53BP1 antibody [EPR2172(2)] (ab237174)](https://www.abcam.com/ps/products/237/ab237174/Images/ab237174-3-benefits-of-recombinant-antibodies.png)
Alexa Fluor® 488 Anti-53BP1 antibody [EPR2172(2)] (ab237174)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab237174 has not yet been referenced specifically in any publications.