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    alexa-fluor-488-aldh1a1-antibody-ep1933y-ab195254.pdf

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RecombinantRabMAb

Recombinant Alexa Fluor® 488 Anti-ALDH1A1 antibody [EP1933Y] (ab195254)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-ALDH1A1 antibody [EP1933Y] (ab195254)
  • Alexa Fluor® 488 Anti-ALDH1A1 antibody [EP1933Y] (ab195254)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [EP1933Y] to ALDH1A1
  • Suitable for: ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

You may also be interested in

Protein
Recombinant Human ALDH1A1 protein (ab173068)
Primary
Product image
Alexa Fluor® 647 Anti-ALDH1A1 antibody [EP1933Y] (ab195255)

View more associated products

Overview

  • Product name

    Alexa Fluor® 488 Anti-ALDH1A1 antibody [EP1933Y]
    See all ALDH1A1 primary antibodies
  • Description

    Alexa Fluor® 488 Rabbit monoclonal [EP1933Y] to ALDH1A1
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ALDH1A1 aa 300-400 (C terminal). The exact sequence is proprietary.

  • Positive control

    • ICC/IF: MCF7
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 30% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1933Y
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Parkinson's disease
    • Other
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Stem Cells
    • Hematopoietic Progenitors
    • Intracellular Molecules
    • Cancer
    • Tumor biomarkers
    • Other

Associated products

  • Alternative Versions

    • Alexa Fluor® 647 Anti-ALDH1A1 antibody [EP1933Y] (ab195255)
    • HRP Anti-ALDH1A1 antibody [EP1933Y] (ab195517)
    • Alexa Fluor® 594 Anti-ALDH1A1 antibody [EP1933Y] (ab206884)
    • PE Anti-ALDH1A1 antibody [EP1933Y] (ab209437)
    • Anti-ALDH1A1 antibody [EP1933Y] - BSA and Azide free (ab215996)
    • Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)
  • Isotype control

    • Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)
  • Recombinant Protein

    • Recombinant Human ALDH1A1 protein (ab173068)

Applications

Our Abpromise guarantee covers the use of ab195254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50.

Target

  • Function

    Binds free retinal and cellular retinol-binding protein-bound retinal. Can convert/oxidize retinaldehyde to retinoic acid.
  • Pathway

    Cofactor metabolism; retinol metabolism.
  • Sequence similarities

    Belongs to the aldehyde dehydrogenase family.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession P00352 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 216 Human
    • Entrez Gene: 11668 Mouse
    • Entrez Gene: 24188 Rat
    • Omim: 100640 Human
    • SwissProt: P00352 Human
    • SwissProt: P24549 Mouse
    • SwissProt: P51647 Rat
    • Unigene: 76392 Human
    • Unigene: 250866 Mouse
    • Unigene: 6132 Rat
    see all
  • Alternative names

    • Acetaldehyde dehydrogenase 1 antibody
    • AHD2 antibody
    • AL1A1_HUMAN antibody
    • ALDC antibody
    • Aldehyde dehydrogenase 1 family member A1 antibody
    • Aldehyde dehydrogenase 1 soluble antibody
    • Aldehyde dehydrogenase 1A1 antibody
    • Aldehyde dehydrogenase antibody
    • Aldehyde dehydrogenase cytosolic antibody
    • Aldehyde dehydrogenase family 1 member A1 antibody
    • Aldehyde dehydrogenase liver cytosolic antibody
    • ALDH 1 antibody
    • ALDH 1A1 antibody
    • ALDH class 1 antibody
    • ALDH, liver cytosolic antibody
    • ALDH-E1 antibody
    • ALDH1 A1 antibody
    • ALDH1 antibody
    • ALDH11 antibody
    • ALDH1A1 antibody
    • ALHDII antibody
    • cytosolic antibody
    • epididymis luminal protein 12 antibody
    • epididymis luminal protein 9 antibody
    • epididymis secretory sperm binding protein Li 53e antibody
    • HEL-S-53e antibody
    • MGC2318 antibody
    • PUMB1 antibody
    • RALDH 1 antibody
    • RalDH1 antibody
    • Retinal dehydrogenase 1 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-ALDH1A1 antibody [EP1933Y] (ab195254)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-ALDH1A1 antibody [EP1933Y] (ab195254)

    ab195254 staining ALDH1A1 in MCF7 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab195254 at a working dilution of 1 in 100 (shown in green) and ab195889, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 594, shown in red) at 2µg/ml overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Alexa Fluor® 488 Anti-ALDH1A1 antibody [EP1933Y] (ab195254)
    Alexa Fluor® 488 Anti-ALDH1A1 antibody [EP1933Y] (ab195254)

Protocols

  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab195254? Please let us know so that we can cite the reference in this datasheet.

    ab195254 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    There are currently no Customer reviews or Questions for ab195254.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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