Recombinant Alexa Fluor® 488 Anti-ALDH1A1 antibody [EP1933Y] (ab195254)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 488 Rabbit monoclonal [EP1933Y] to ALDH1A1
- Suitable for: ICC/IF
- Reacts with: Mouse, Rat, Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Overview
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Product name
Alexa Fluor® 488 Anti-ALDH1A1 antibody [EP1933Y]
See all ALDH1A1 primary antibodies -
Description
Alexa Fluor® 488 Rabbit monoclonal [EP1933Y] to ALDH1A1 -
Host species
Rabbit -
Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human ALDH1A1 aa 300-400 (C terminal). The exact sequence is proprietary.
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Positive control
- ICC/IF: MCF7
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol, 1% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1933Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Alexa Fluor® 647 Anti-ALDH1A1 antibody [EP1933Y] (ab195255)
- HRP Anti-ALDH1A1 antibody [EP1933Y] (ab195517)
- Alexa Fluor® 594 Anti-ALDH1A1 antibody [EP1933Y] (ab206884)
- PE Anti-ALDH1A1 antibody [EP1933Y] (ab209437)
- Anti-ALDH1A1 antibody [EP1933Y] - BSA and Azide free (ab215996)
- Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab195254 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | 1/50. |
Target
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Function
Binds free retinal and cellular retinol-binding protein-bound retinal. Can convert/oxidize retinaldehyde to retinoic acid. -
Pathway
Cofactor metabolism; retinol metabolism. -
Sequence similarities
Belongs to the aldehyde dehydrogenase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 216 Human
- Entrez Gene: 11668 Mouse
- Entrez Gene: 24188 Rat
- Omim: 100640 Human
- SwissProt: P00352 Human
- SwissProt: P24549 Mouse
- SwissProt: P51647 Rat
- Unigene: 76392 Human
see all -
Alternative names
- Acetaldehyde dehydrogenase 1 antibody
- AHD2 antibody
- AL1A1_HUMAN antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-ALDH1A1 antibody [EP1933Y] (ab195254)
ab195254 staining ALDH1A1 in MCF7 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab195254 at a working dilution of 1 in 100 (shown in green) and ab195889, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 594, shown in red) at 2µg/ml overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Protocols
Datasheets and documents
Certificate of Compliance
References (0)
ab195254 has not yet been referenced specifically in any publications.