Alexa Fluor® 488 Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab197496)
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab197496)](https://www.abcam.com/ps/products/197/ab197496/Images/ab197496-277531-ICCAP22193302.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab197496)")
Key features and details
- Alexa Fluor® 488 Mouse monoclonal [464.6] to alpha 1 Sodium Potassium ATPase
- Suitable for: ICC/IF
- Reacts with: Mouse, Rat, Sheep, Rabbit, Dog, Human, Pig, Xenopus laevis, Monkey
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Overview
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Product name
Alexa Fluor® 488 Anti-alpha 1 Sodium Potassium ATPase antibody [464.6]
See all alpha 1 Sodium Potassium ATPase primary antibodies -
Description
Alexa Fluor® 488 Mouse monoclonal [464.6] to alpha 1 Sodium Potassium ATPase -
Host species
Mouse -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Sheep, Rabbit, Dog, Human, Pig, Xenopus laevis, Monkey -
Immunogen
Full length native protein (purified) corresponding to Rabbit alpha 1 Sodium Potassium ATPase. Full length native protein purified from rabbit renal outermedulla.
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Positive control
- ICC/IF: HEK293 cells.
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General notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
464.6 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab197496 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100 - 1/250. This product gave a positive signal in Hek293 cells fixed with 100% methanol (5 min) |
Target
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Function
This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. -
Sequence similarities
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily. -
Post-translational
modificationsPhosphorylation on Tyr-10 modulates pumping activity. -
Cellular localization
Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 403992 Dog
- Entrez Gene: 476 Human
- Entrez Gene: 11928 Mouse
- Entrez Gene: 397481 Pig
- Entrez Gene: 24211 Rat
- Entrez Gene: 443381 Sheep
- Entrez Gene: 399285 Xenopus laevis
- Omim: 182310 Human
see all -
Alternative names
- A1A1 antibody
- AT1A1 antibody
- AT1A1_HUMAN antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab197496)
ab197496 staining alpha 1 Sodium Potassium ATPase in Hek293 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab197496 at 1/250 dilution (shown in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Protocols
Datasheets and documents
References (3)
ab197496 has been referenced in 3 publications.
- Roy G et al. A novel bicistronic gene design couples stable cell line selection with a fucose switch in a designer CHO host to produce native and afucosylated glycoform antibodies. MAbs 10:416-430 (2018). PubMed: 29400603
- Yu SM et al. Substrate curvature affects the shape, orientation, and polarization of renal epithelial cells. Acta Biomater N/A:N/A (2018). PubMed: 30006316
- Honda K et al. Molecular architecture underlying fluid absorption by the developing inner ear. Elife 6:N/A (2017). IHC ; Mouse . PubMed: 28994389
