Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (ab184675)
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (ab184675)](https://www.abcam.com/ps/products/184/ab184675/Images/ab184675-397130-Project20200622-ab184675-AP5612333-2ug-sv40ltsmcm-DS-image2.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (ab184675)")
Key features and details
- Alexa Fluor® 488 Mouse monoclonal [1A4] to alpha smooth muscle Actin
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Rat
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
- Isotype: IgG2a
Overview
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Product name
Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4]
See all alpha smooth muscle Actin primary antibodies -
Description
Alexa Fluor® 488 Mouse monoclonal [1A4] to alpha smooth muscle Actin -
Host species
Mouse -
Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
Tested applications
Suitable for: Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Mouse, Sheep, Goat, Chicken, Guinea pig, Cat, Dog, Human, Snake, Zebrafish
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Positive control
- ICC/IF: SV40LT-SMC cells. Flow Cyt: SV40LT-SMC cells.
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General notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
1A4 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
- Biotin Anti-alpha smooth muscle Actin antibody [1A4] (ab125057)
- Alexa Fluor® 594 Anti-alpha smooth muscle Actin antibody [1A4] (ab202368)
- HRP Anti-alpha smooth muscle Actin antibody [1A4] (ab203696)
- Anti-alpha smooth muscle Actin antibody [1A4] - BSA and Azide free (ab240654)
- Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)
- FITC Anti-alpha smooth muscle Actin antibody [1A4] (ab8211)
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab184675 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/100. ab171464 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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| ICC/IF | 1/100. |
Target
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Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. -
Involvement in disease
Defects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance. -
Sequence similarities
Belongs to the actin family. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 101093547 Cat
- Entrez Gene: 423787 Chicken
- Entrez Gene: 477587 Dog
- Entrez Gene: 59 Human
- Entrez Gene: 11475 Mouse
- Entrez Gene: 81633 Rat
- Entrez Gene: 101121051 Sheep
- Entrez Gene: 322509 Zebrafish
see all -
Alternative names
- a actin antibody
- AAT6 antibody
- ACTA_HUMAN antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (ab184675)
ab184675 staining alpha Smooth Muscle Actin in SV40LT-SMC cells. The cells were fixed with 100% Methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab184675 at 1/250 dilution (shown in green) and ab195884, Rat monoclonal to alpha Tubulin (Alexa Fluor® 647), at 2µg/ml (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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![Flow Cytometry - Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (ab184675)](https://www.abcam.com/ps/products/184/ab184675/Images/ab184675-239508-anti-alpha-smooth-muscle-actin-antibody-1a4-alexa-fluor-488-flow-cytometry.jpg)
Flow Cytometry - Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (ab184675)Overlay histogram showing SV40LT-SMC cells stained with ab184675 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab184675, 1/50 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
This antibody gave a positive signal in SV40LT-SMC fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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![Alexa Fluor® - Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (ab184675)](https://www.abcam.com/ps/products/184/ab184675/Images/ab184675-217870-AlexaEmissionSpectrumDS.jpg)
Alexa Fluor® - Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (ab184675)Emission spectra of various Alexa Fluor® conjugates
Protocols
References (7)
ab184675 has been referenced in 7 publications.
- Deng J et al. Single-cell gene profiling and lineage tracing analyses revealed novel mechanisms of endothelial repair by progenitors. Cell Mol Life Sci N/A:N/A (2020). PubMed: 32166394
- Mahmoudi S et al. Heterogeneity in old fibroblasts is linked to variability in reprogramming and wound healing. Nature 574:553-558 (2019). PubMed: 31645721
- Zhang FP et al. Lack of androgen receptor SUMOylation results in male infertility due to epididymal dysfunction. Nat Commun 10:777 (2019). PubMed: 30770815
- Ritsma L et al. Integrin ß1 activation induces an anti-melanoma host response. PLoS One 12:e0175300 (2017). IHC, ICC/IF ; Mouse, Human . PubMed: 28448494
- Ikutani M et al. Prolonged activation of IL-5-producing ILC2 causes pulmonary arterial hypertrophy. JCI Insight 2:e90721 (2017). PubMed: 28405615
- Woeller CF et al. The Aryl Hydrocarbon Receptor and Its Ligands Inhibit Myofibroblast Formation and Activation: Implications for Thyroid Eye Disease. Am J Pathol 186:3189-3202 (2016). PubMed: 27842700
- Wang X et al. Hsp20-Mediated Activation of Exosome Biogenesis in Cardiomyocytes Improves Cardiac Function and Angiogenesis in Diabetic Mice. Diabetes 65:3111-28 (2016). PubMed: 27284111
