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  1. Link

    alexa-fluor-488-alpha-smooth-muscle-actin-antibody-1a4-ab184675.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin
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Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (ab184675)

  • Datasheet
  • SDS
Reviews (6) Submit a question References (10)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (ab184675)
  • Flow Cytometry - Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (ab184675)

Key features and details

  • Alexa Fluor® 488 Mouse monoclonal [1A4] to alpha smooth muscle Actin
  • Suitable for: Flow Cyt, ICC/IF
  • Reacts with: Rat
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Isotype: IgG2a

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4]
    See all alpha smooth muscle Actin primary antibodies
  • Description

    Alexa Fluor® 488 Mouse monoclonal [1A4] to alpha smooth muscle Actin
  • Host species

    Mouse
  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested applications

    Suitable for: Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat
    Predicted to work with: Mouse, Sheep, Goat, Chicken, Guinea pig, Cat, Dog, Human, Snake, Zebrafish
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: SV40LT-SMC cells. Flow Cyt: SV40LT-SMC cells.
  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    1A4
  • Isotype

    IgG2a
  • Light chain type

    kappa
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin
    • Stem Cells
    • Lineage Markers
    • Mesoderm
    • Stem Cells
    • Mesenchymal Stem Cells
    • Myogenesis
    • Cardiovascular
    • Heart
    • Contractility
    • Contractile Proteins
    • Actins
    • Cardiovascular
    • Vasculature
    • Vasculature Markers
    • Arterial
    • Cardiovascular
    • Vasculature
    • Smooth muscle cell (SMC)
    • Cytoskeleton
    • Developmental Biology
    • Lineage specification
    • Mesoderm
    • Cardiovascular
    • Cardiovascular Markers
    • Cell Markers
    • Smooth Muscle Cells

Associated products

  • Alternative Versions

    • Biotin Anti-alpha smooth muscle Actin antibody [1A4] (ab125057)
    • Alexa Fluor® 594 Anti-alpha smooth muscle Actin antibody [1A4] (ab202368)
    • HRP Anti-alpha smooth muscle Actin antibody [1A4] (ab203696)
    • Anti-alpha smooth muscle Actin antibody [1A4] - BSA and Azide free (ab240654)
    • Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)
    • FITC Anti-alpha smooth muscle Actin antibody [1A4] (ab8211)
  • Isotype control

    • Alexa Fluor® 488 Mouse IgG2a monoclonal [PPV-04] - Isotype Control (ab237608)
  • Recombinant Protein

    • Recombinant Human alpha smooth muscle Actin protein (ab134555)
  • Related Products

    • Phalloidin-iFluor 488 Reagent (ab176753)
    • Phalloidin-iFluor 647 Reagent (ab176759)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab184675 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
1/100.

ab171464 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ICC/IF
1/100.
Notes
Flow Cyt
1/100.

ab171464 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ICC/IF
1/100.

Target

  • Function

    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in disease

    Defects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.
  • Sequence similarities

    Belongs to the actin family.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Target information above from: UniProt accession P62736 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 101093547 Cat
    • Entrez Gene: 423787 Chicken
    • Entrez Gene: 477587 Dog
    • Entrez Gene: 59 Human
    • Entrez Gene: 11475 Mouse
    • Entrez Gene: 81633 Rat
    • Entrez Gene: 101121051 Sheep
    • Entrez Gene: 322509 Zebrafish
    • Omim: 102620 Human
    • SwissProt: P08023 Chicken
    • SwissProt: P62736 Human
    • SwissProt: P62737 Mouse
    • SwissProt: P62738 Rat
    • Unigene: 500483 Human
    • Unigene: 213025 Mouse
    • Unigene: 195319 Rat
    • Unigene: 3114 Rat
    see all
  • Alternative names

    • a actin antibody
    • AAT6 antibody
    • ACTA_HUMAN antibody
    • ACTA2 antibody
    • Actin alpha 2 smooth muscle aorta antibody
    • Actin aortic smooth muscle antibody
    • Actin, aortic smooth muscle antibody
    • ACTSA antibody
    • ACTVS antibody
    • Alpha 2 actin antibody
    • Alpha actin 2 antibody
    • Alpha cardiac actin antibody
    • alpha sma antibody
    • Alpha-actin-2 antibody
    • Cell growth inhibiting gene 46 protein antibody
    • Cell growth-inhibiting gene 46 protein antibody
    • GIG46 antibody
    • Growth inhibiting gene 46 antibody
    • MYMY5 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (ab184675)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (ab184675)

    ab184675 staining alpha Smooth Muscle Actin in SV40LT-SMC cells. The cells were fixed with 100% Methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab184675 at 1/250 dilution (shown in green) and ab195884, Rat monoclonal to alpha Tubulin (Alexa Fluor® 647), at 2µg/ml (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Flow Cytometry - Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (ab184675)
    Flow Cytometry - Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (ab184675)

    Overlay histogram showing SV40LT-SMC cells stained with ab184675 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab184675, 1/50 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    This antibody gave a positive signal in SV40LT-SMC fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Protocols

  • Flow cytometry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (10)

Publishing research using ab184675? Please let us know so that we can cite the reference in this datasheet.

ab184675 has been referenced in 10 publications.

  • James J  et al. Single Mutation in the NFU1 Gene Metabolically Reprograms Pulmonary Artery Smooth Muscle Cells. Arterioscler Thromb Vasc Biol 41:734-754 (2021). PubMed: 33297749
  • Deng J  et al. Single-cell gene profiling and lineage tracing analyses revealed novel mechanisms of endothelial repair by progenitors. Cell Mol Life Sci N/A:N/A (2020). PubMed: 32166394
  • Kenney HM  et al. Lineage tracing reveals evidence of a popliteal lymphatic muscle progenitor cell that is distinct from skeletal and vascular muscle progenitors. Sci Rep 10:18088 (2020). PubMed: 33093635
  • Ruehle MA  et al. Extracellular matrix compression temporally regulates microvascular angiogenesis. Sci Adv 6:N/A (2020). PubMed: 32937368
  • Mahmoudi S  et al. Heterogeneity in old fibroblasts is linked to variability in reprogramming and wound healing. Nature 574:553-558 (2019). PubMed: 31645721
  • Zhang FP  et al. Lack of androgen receptor SUMOylation results in male infertility due to epididymal dysfunction. Nat Commun 10:777 (2019). PubMed: 30770815
  • Ritsma L  et al. Integrin ß1 activation induces an anti-melanoma host response. PLoS One 12:e0175300 (2017). IHC, ICC/IF ; Mouse, Human . PubMed: 28448494
  • Ikutani M  et al. Prolonged activation of IL-5-producing ILC2 causes pulmonary arterial hypertrophy. JCI Insight 2:e90721 (2017). PubMed: 28405615
  • Woeller CF  et al. The Aryl Hydrocarbon Receptor and Its Ligands Inhibit Myofibroblast Formation and Activation: Implications for Thyroid Eye Disease. Am J Pathol 186:3189-3202 (2016). PubMed: 27842700
  • Wang X  et al. Hsp20-Mediated Activation of Exosome Biogenesis in Cardiomyocytes Improves Cardiac Function and Angiogenesis in Diabetic Mice. Diabetes 65:3111-28 (2016). PubMed: 27284111

Customer reviews and Q&As

Show All Reviews Q&A
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1-6 of 6 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization
No
Specification
colon
Blocking step
Intercept as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: RT°C
Fixative
10% NBF
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 07 2022

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mouse Lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 1 mM EDTA, pH 8.0
Permeabilization
Yes - 0.5% Tween 20
Specification
Mouse Lung
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Helen Stoelting

Verified customer

Submitted Feb 03 2022

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Fibroid Tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6 & TRIS pH 9
Permeabilization
Yes - 0.3% Triton X-100
Specification
Fibroid Tissue
Blocking step
Serum as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Dylan Windell

Verified customer

Submitted May 12 2021

IHC - Wholemount abreview for Anti-alpha smooth muscle Actin antibody [1A4] (Alexa Fluor® 488)

Excellent
Abreviews
Abreviews
abreview image
Application
IHC - Wholemount
Sample
Human Tissue (Colon / colonic lymphoid tissue)
Specification
Colon / colonic lymphoid tissue
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 14 2020

Immunohistochemistry (Frozen sections) abreview for Anti-alpha smooth muscle Actin antibody [1A4] (Alexa Fluor® 488)

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (fetal lung)
Permeabilization
No
Specification
fetal lung
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 15 2016

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-alpha smooth muscle Actin [1A4] antibody (Alexa Fluor® 488)

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM citric buffer (PH 6.0), boiling for 20 minutes
Sample
Mouse Tissue sections (Intestine tissue)
Specification
Intestine tissue
Permeabilization
Yes - 0.2% Triton-100 in PBS
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 03 2014

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