Alexa Fluor® 488 Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab197904)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab197904)

    Key features and details

    • Alexa Fluor® 488 Mouse monoclonal [3D5] to ATPB - Mitochondrial Marker
    • Suitable for: ICC/IF
    • Reacts with: Mouse, Rat, Cow, Human, Caenorhabditis elegans, Monkey
    • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
    • Isotype: IgG1

    Overview

    • Product name

      Alexa Fluor® 488 Anti-ATPB antibody [3D5] - Mitochondrial Marker
      See all ATPB primary antibodies

    • Description

      Alexa Fluor® 488 Mouse monoclonal [3D5] to ATPB - Mitochondrial Marker

    • Host species

      Mouse

    • Conjugation

      Alexa Fluor® 488. Ex: 495nm, Em: 519nm

    • Tested applications

      Suitable for: ICC/IFmore details

    • Species reactivity

      Reacts with: Mouse, Rat, Cow, Human, Caenorhabditis elegans, Monkey

    • Immunogen

      Tissue, cells or virus corresponding to Human ATPB.

    • Positive control

      • ICC/IF: HepG2 cells.

    • General notes

       

       

      Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

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    Properties

    Applications

    Our Abpromise guarantee covers the use of ab197904 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF 1/100.

    This product gave a positive signal in HepG2 cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).

    Target

    • Function

      Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.

    • Sequence similarities

      Belongs to the ATPase alpha/beta chains family.

    • Cellular localization

      Mitochondrion. Mitochondrion inner membrane. Peripheral membrane protein.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • ATP 5B antibody
      • ATP synthase H+ transporting mitochondrial F1 complex beta polypeptide antibody
      • ATP synthase subunit beta mitochondrial antibody
      • ATP synthase subunit beta, mitochondrial antibody
      • atp5b antibody
      • ATPB antibody
      • ATPB_HUMAN antibody
      • ATPMB antibody
      • ATPSB antibody
      • Epididymis secretory protein Li 271 antibody
      • HEL-S-271 antibody
      • Mitochondrial ATP synthase beta subunit antibody
      • Mitochondrial ATP Synthase Subunit Beta antibody
      • Mitochondrial ATP synthetase beta subunit antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab197904)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab197904)

      ab197904 staining ATPB in HepG2 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab197904 at a 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      This product also gave a positive signal under the same testing conditions in HepG2 cells fixed with 100% methanol (5 min)

    References (1)

    Publishing research using ab197904? Please let us know so that we can cite the reference in this datasheet.

    ab197904 has been referenced in 1 publication.

    • Liu Y  et al. Eyes shut homolog (EYS) interacts with matriglycan of O-mannosyl glycans whose deficiency results in EYS mislocalization and degeneration of photoreceptors. Sci Rep 10:7795 (2020). PubMed: 32385361

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