Validated using a knockout cell line

Alexa Fluor® 488 Anti-beta III Tubulin antibody [2G10] (ab195879)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-beta III Tubulin antibody [2G10] (ab195879)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-beta III Tubulin antibody [2G10] (ab195879)
  • Flow Cytometry - Alexa Fluor® 488 Anti-beta III Tubulin antibody [2G10] (ab195879)

Key features and details

  • Alexa Fluor® 488 Mouse monoclonal [2G10] to beta III Tubulin
  • Suitable for: Flow Cyt, ICC/IF
  • Knockout validated
  • Reacts with: Rat, Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Isotype: IgG2a

Overview

  • Product name

    Alexa Fluor® 488 Anti-beta III Tubulin antibody [2G10]
    See all beta III Tubulin primary antibodies

  • Description

    Alexa Fluor® 488 Mouse monoclonal [2G10] to beta III Tubulin

  • Host species

    Mouse

  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

  • Tested applications

    Suitable for: Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Tissue, cells or virus corresponding to Rat beta III Tubulin.

  • Positive control

    • ICC/IF: PC12 cells, HAP1 cells (HAP1-TUBB3 used as negative cell line). Flow Cyt: PC12 cells.

  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

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    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA, 30% Glycerol
  • Concentration information loading...

  • Purity

    IgG fraction

  • Clonality

    Monoclonal

  • Clone number

    2G10

  • Isotype

    IgG2a

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab195879 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/500.

ab171464 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.

Target

  • Function

    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance.

  • Tissue specificity

    Expression is primarily restricted to central and peripheral nervous system.

  • Involvement in disease

    Defects in TUBB3 are the cause of congenital fibrosis of extraocular muscles type 3A (CFEOM3A) [MIM:600638]. A congenital ocular motility disorder marked by restrictive ophthalmoplegia affecting extraocular muscles innervated by the oculomotor and/or trochlear nerves. It is clinically characterized by anchoring of the eyes in downward gaze, ptosis, and backward tilt of the head. Congenital fibrosis of extraocular muscles type 3 presents as a non-progressive, autosomal dominant disorder with variable expression. Patients may be bilaterally or unilaterally affected, and their oculo-motility defects range from complete ophthalmoplegia (with the eyes fixed in a hypo- and exotropic position), to mild asymptomatic restrictions of ocular movement. Ptosis, refractive error, amblyopia, and compensatory head positions are associated with the more severe forms of the disorder. In some cases the ocular phenotype is accompanied by additional features including developmental delay, corpus callosum agenesis, basal ganglia dysmorphism, facial weakness, polyneuropathy.

  • Sequence similarities

    Belongs to the tubulin family.

  • Domain

    The highly acidic C-terminal region may bind cations such as calcium.

  • Post-translational
    modifications

    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.

  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Information by UniProt

  • Database links

    • Alternative names

      • beta 3 tubulin antibody
      • beta 4 antibody
      • beta-4 antibody
      • CDCBM antibody
      • CDCBM1 antibody
      • CFEOM3 antibody
      • CFEOM3A antibody
      • FEOM3 antibody
      • M(beta)3 antibody
      • M(beta)6 antibody
      • MC1R antibody
      • Neuron specific beta III Tubulin antibody
      • Neuron-specific class III beta-tubulin antibody
      • QccE-11995 antibody
      • QccE-15186 antibody
      • TBB3_HUMAN antibody
      • Tubb 3 antibody
      • TUBB3 antibody
      • TUBB4 antibody
      • Tubulin beta 3 antibody
      • Tubulin beta 3 chain antibody
      • Tubulin beta 4 antibody
      • Tubulin beta III antibody
      • Tubulin beta-3 chain antibody
      • Tubulin beta-4 chain antibody
      • Tubulin beta-III antibody
      • tuj 1 antibody
      • tuj1 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-beta III Tubulin antibody [2G10] (ab195879)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-beta III Tubulin antibody [2G10] (ab195879)

      ab195879 staining beta III tubulin (shown in green) in wild-type HAP1 cells (top panel) and TUBB3 knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab195879 at 1/500 dilution (shown in green) and ab195889 at 1/250 dilution (shown in pseudo colour red) overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-beta III Tubulin antibody [2G10] (ab195879)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-beta III Tubulin antibody [2G10] (ab195879)

      ab195879 staining beta III Tubulin in PC12 cells. The cells were fixed with 100% methanol (5 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab195879 at 1/100 dilution (shown in green) overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Flow Cytometry - Alexa Fluor® 488 Anti-beta III Tubulin antibody [2G10] (ab195879)
      Flow Cytometry - Alexa Fluor® 488 Anti-beta III Tubulin antibody [2G10] (ab195879)
      Overlay histogram showing PC12 cells stained with ab195879 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab195879, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

      Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

      This antibody gave a positive signal in PC12 cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    References (3)

    Publishing research using ab195879? Please let us know so that we can cite the reference in this datasheet.

    ab195879 has been referenced in 3 publications.

    • Ostasov P  et al. Sonic Hedgehog and Triiodothyronine Pathway Interact in Mouse Embryonic Neural Stem Cells. Int J Mol Sci 21:N/A (2020). PubMed: 32456161
    • Li Q  et al. Role of catalpol in ameliorating the pathogenesis of experimental autoimmune encephalomyelitis by increasing the level of noradrenaline in the locus coeruleus. Mol Med Rep 17:4163-4172 (2018). PubMed: 29328415
    • Cui Y  et al. The miR-20-Rest-Wnt signaling axis regulates neural progenitor cell differentiation. Sci Rep 6:23300 (2016). Flow Cyt ; Rat . PubMed: 26996236

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