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  1. Link

    alexa-fluor-488-c-myc-antibody-y69-ab190026.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families HLH / Leucine Zipper HLH / Leucine Zipper
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Alexa Fluor® 488 Anti-c-Myc antibody [Y69] (ab190026)

  • Datasheet
  • SDS
  • Certificate of Compliance
Submit a review Submit a question References (3)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-c-Myc antibody [Y69] (ab190026)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-c-Myc antibody [Y69] (ab190026)
  • Alexa Fluor® 488 Anti-c-Myc antibody [Y69] (ab190026)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [Y69] to c-Myc
  • Suitable for: ICC/IF
  • Knockout validated
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free FITC HRP Unconjugated

You may also be interested in

Primary
Product image
Anti-c-Myc antibody [Y69] - ChIP Grade - BSA and Azide free (ab168727)
Protein
Recombinant human c-Myc protein (Active) (ab169901)
Conjugation
Product image
Alexa Fluor® 488 Conjugation Kit (Fast) - Lightning-Link® (ab236553)

View more associated products

Overview

  • Product name

    Alexa Fluor® 488 Anti-c-Myc antibody [Y69]
    See all c-Myc primary antibodies
  • Description

    Alexa Fluor® 488 Rabbit monoclonal [Y69] to c-Myc
  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab166837)

  • Positive control

    • ICC/IF: Panc1 and HEK293 cells.
  • General notes

    If you need other conjugated anti-c-myc (Y69) RabMAb antibodies, find our range of products here.

    We also offer a PBS only version of this clone as product ab168727.

    For more information on choosing the right c-Myc antibody for you, please click here.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Dissociation constant (KD)

    KD = 3.80 x 10 -12 M
    Learn more about KD
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    Y69
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • HLH / Leucine Zipper
    • Stem Cells
    • Signaling Pathways
    • TGF beta
    • Nuclear
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Cancer
    • Cell cycle
    • Cell differentiation
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Transcription factors
    • Cancer
    • Tumor biomarkers
    • Oncoproteins

Associated products

  • Alternative Versions

    • Anti-c-Myc antibody [Y69] - ChIP Grade - BSA and Azide free (ab168727)
    • Alexa Fluor® 594 Anti-c-Myc antibody [Y69] (ab201775)
    • Alexa Fluor® 555 Anti-c-Myc antibody [Y69] (ab201780)
    • HRP Anti-c-Myc antibody [Y69] (ab205818)
    • FITC Anti-c-Myc antibody [Y69] (ab223913)
    • Anti-c-Myc antibody [Y69] - ChIP Grade (ab32072)
  • Assay kits

    • c-Myc Transcription Factor Assay Kit (Colorimetric) (ab207200)
  • Immunizing Peptide (Blocking)

    • Human c-Myc peptide (ab166837)
  • Isotype control

    • Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)
  • KO cell lines

    • Human MYC (c-Myc) knockout HEK-293T cell line (ab256500)
  • Positive Controls

    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant human c-Myc protein (Active) (ab169901)
  • Related Products

    • Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab190026 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/50 - 1/100.

This product gave a positive signal in Panc1 cells fixed with 4% formaldehyde (10 min)

Notes
ICC/IF
1/50 - 1/100.

This product gave a positive signal in Panc1 cells fixed with 4% formaldehyde (10 min)

Target

  • Function

    Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.
  • Involvement in disease

    Note=Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors.
    Note=A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
    Defects in MYC are a cause of Burkitt lymphoma (BL) [MIM:113970]. A form of undifferentiated malignant lymphoma commonly manifested as a large osteolytic lesion in the jaw or as an abdominal mass. Note=Chromosomal aberrations involving MYC are usually found in Burkitt lymphoma. Translocations t(8;14), t(8;22) or t(2;8) which juxtapose MYC to one of the heavy or light chain immunoglobulin gene loci.
  • Sequence similarities

    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Post-translational
    modifications

    Phosphorylated by PRKDC. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome.
    Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex.
  • Cellular localization

    Nucleus > nucleoplasm. Nucleus > nucleolus.
  • Target information above from: UniProt accession P01106 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4609 Human
    • Entrez Gene: 17869 Mouse
    • Entrez Gene: 24577 Rat
    • Omim: 190080 Human
    • SwissProt: P01106 Human
    • SwissProt: P01108 Mouse
    • SwissProt: P09416 Rat
    • Unigene: 202453 Human
    • Unigene: 2444 Mouse
    • Unigene: 12072 Rat
    see all
  • Form

    c-Myc is also expressed in the cytoplasm.
  • Alternative names

    • AU016757 antibody
    • Avian myelocytomatosis viral oncogene homolog antibody
    • bHLHe39 antibody
    • c Myc antibody
    • Cellular myelocytomatosis oncogene antibody
    • Class E basic helix-loop-helix protein 39 antibody
    • MGC105490 antibody
    • MRTL antibody
    • Myc antibody
    • Myc protein antibody
    • Myc proto oncogene protein antibody
    • Myc proto-oncogene protein antibody
    • myc-related translation/localization regulatory factor antibody
    • MYC_HUMAN antibody
    • Myc2 antibody
    • myca antibody
    • MYCC antibody
    • Myelocytomatosis oncogene a antibody
    • Myelocytomatosis oncogene antibody
    • Niard antibody
    • Nird antibody
    • oncogene c-Myc antibody
    • Oncogene Myc antibody
    • OTTHUMP00000158589 antibody
    • OTTHUMP00000227763 antibody
    • Proto-oncogene c-Myc antibody
    • Protooncogene homologous to myelocytomatosis virus antibody
    • RNCMYC antibody
    • Transcription factor p64 antibody
    • Transcriptional regulator Myc-A antibody
    • V-Myc avian myelocytomatosis viral oncogene homolog antibody
    • v-myc myelocytomatosis viral oncogene homolog (avian) antibody
    • zc-myc antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-c-Myc antibody [Y69] (ab190026)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-c-Myc antibody [Y69] (ab190026)

    ab190026 staining c-Myc in wild-type HEK293 cells (top panel) and MYC knockout HEK293 cells (ab256500) (bottom panel). The cells were fixed with 4% paraformaldehyde (10 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab190026 at 1/5000 dilution and ab190573 (Rabbit monoclonal to alpha Tubulin - Alexa Fluor® 647) at 1/250 dilution overnight at 4°C. Nuclear DNA was labelled in blue with DAPI.
    Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-c-Myc antibody [Y69] (ab190026)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-c-Myc antibody [Y69] (ab190026)

    ab190026 staining c-myc in Panc-1 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab190026 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Alexa Fluor® 488 Anti-c-Myc antibody [Y69] (ab190026)
    Alexa Fluor® 488 Anti-c-Myc antibody [Y69] (ab190026)

Protocols

  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (3)

Publishing research using ab190026? Please let us know so that we can cite the reference in this datasheet.

ab190026 has been referenced in 3 publications.

  • Zhang R  et al. LncRNA MIR31HG is activated by STAT1 and facilitates glioblastoma cell growth via Wnt/ß-catenin signaling pathway. Neurosci Res N/A:N/A (2021). PubMed: 33940081
  • Wang RT  et al. LINC00501 Inhibits the Growth and Metastasis of Lung Cancer by Mediating miR-129-5p/HMGB1. Onco Targets Ther 13:7137-7149 (2020). PubMed: 32801746
  • Jia XX  et al. Wnt/ß-catenin signaling pathway regulates asthma airway remodeling by influencing the expression of c-Myc and cyclin D1 via the p38 MAPK-dependent pathway. Exp Ther Med 18:3431-3438 (2019). PubMed: 31602218

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