Recombinant Alexa Fluor® 488 Anti-c-Myc (phospho T58) antibody [EPR17923] (ab300546)
![Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-c-Myc (phospho T58) antibody [EPR17923] (AB300546)](https://www.abcam.com/ps/products/300/ab300546/Images/AB300546-1-anti-cmyc-phospho-t58-antibody-epr17923-flow-cytometry-hela1.jpg "Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-c-Myc (phospho T58) antibody [EPR17923] (AB300546)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 488 Rabbit monoclonal [EPR17923] to c-Myc (phospho T58)
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Related conjugates and formulations
Overview
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Product name
Alexa Fluor® 488 Anti-c-Myc (phospho T58) antibody [EPR17923]
See all c-Myc primary antibodies -
Description
Alexa Fluor® 488 Rabbit monoclonal [EPR17923] to c-Myc (phospho T58) -
Host species
Rabbit -
Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
Tested applications
Suitable for: Flow Cyt (Intra)more details
Unsuitable for: ICC -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt (Intra): HeLa (Human cervix adenocarcinoma epithelial cell) treated with 200nM Calyculin A and 1uM Okadaic Acid.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17923 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab300546 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/500.
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| Notes |
|---|
|
Flow Cyt (Intra)
1/500. |
Target
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Function
Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes. -
Involvement in disease
Note=Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors.
Note=A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
Defects in MYC are a cause of Burkitt lymphoma (BL) [MIM:113970]. A form of undifferentiated malignant lymphoma commonly manifested as a large osteolytic lesion in the jaw or as an abdominal mass. Note=Chromosomal aberrations involving MYC are usually found in Burkitt lymphoma. Translocations t(8;14), t(8;22) or t(2;8) which juxtapose MYC to one of the heavy or light chain immunoglobulin gene loci. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Post-translational
modificationsPhosphorylated by PRKDC. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome.
Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex. -
Cellular localization
Nucleus > nucleoplasm. Nucleus > nucleolus. - Information by UniProt
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Database links
- Entrez Gene: 4609 Human
- Omim: 190080 Human
- SwissProt: P01106 Human
- Unigene: 202453 Human
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Form
c-Myc is also expressed in the cytoplasm. -
Alternative names
- AU016757 antibody
- Avian myelocytomatosis viral oncogene homolog antibody
- bHLHe39 antibody
see all
Images
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Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-c-Myc (phospho T58) antibody [EPR17923] (AB300546)
Flow cytometric (Intracellular) analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells treated with 200nM Calyculin A and 1µM Okadaic Acid for 60 min (Red) / Untreated control (Green) cells labeling c-Myc (phospho T58) with ab300546 at 1/500 dilution (0.1µg) (Red) and Green (Red) compared with a Rabbit monoclonal isotype control - Alexa Fluor® 488 (Black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue).
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![Alexa Fluor® 488 Anti-c-Myc (phospho T58) antibody [EPR17923] (AB300546)](https://www.abcam.com/ps/products/300/ab300546/Images/AB300546-2-Benefits-of-Recombinant-antibodies.jpg)
Alexa Fluor® 488 Anti-c-Myc (phospho T58) antibody [EPR17923] (AB300546)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab300546 has not yet been referenced specifically in any publications.