RecombinantRabMAb

Recombinant Alexa Fluor® 488 Anti-Cardiac Troponin I antibody [EP1106Y] (ab196384)

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Flow Cytometry - Alexa Fluor® 488 Anti-Cardiac Troponin I antibody [EP1106Y] (ab196384)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cardiac Troponin I antibody [EP1106Y] (ab196384)
  • Alexa Fluor® 488 Anti-Cardiac Troponin I antibody [EP1106Y] (ab196384)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [EP1106Y] to Cardiac Troponin I
  • Suitable for: ICC/IF, Flow Cyt
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Overview

  • Product name

    Alexa Fluor® 488 Anti-Cardiac Troponin I antibody [EP1106Y]
    See all Cardiac Troponin I primary antibodies

  • Description

    Alexa Fluor® 488 Rabbit monoclonal [EP1106Y] to Cardiac Troponin I

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

  • Tested applications

    Suitable for: ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human Cardiac Troponin I (N terminal). The exact sequence is proprietary.

  • Positive control

    • ICC/IF: A673 cells Flow Cyt: A673 cells.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab196384 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
Flow Cyt 1/50.

Target

  • Function

    Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

  • Involvement in disease

    Defects in TNNI3 are the cause of cardiomyopathy familial hypertrophic type 7 (CMH7) [MIM:613690]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in TNNI3 are the cause of cardiomyopathy familial restrictive type 1 (RCM1) [MIM:115210]. RCM1 is an heart muscle disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.
    Defects in TNNI3 are the cause of cardiomyopathy dilated type 2A (CMD2A) [MIM:611880]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in TNNI3 are the cause of cardiomyopathy dilated type 1FF (CMD1FF) [MIM:613286]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.

  • Sequence similarities

    Belongs to the troponin I family.
  • Information by UniProt

  • Database links

    • Alternative names

      • cardiac muscle antibody
      • Cardiac troponin I antibody
      • cardiomyopathy, dilated 2A (autosomal recessive) antibody
      • Cardiomyopathy, familial hypertrophic, 7, included antibody
      • CMD1FF antibody
      • CMD2A antibody
      • CMH7 antibody
      • cTnI antibody
      • Familial hypertrophic cardiomyopathy 7 antibody
      • MGC116817 antibody
      • RCM1 antibody
      • Tn1 antibody
      • Tni antibody
      • TNN I3 antibody
      • TNNC 1 antibody
      • TNNC1 antibody
      • TNNI3 antibody
      • TNNI3_HUMAN antibody
      • Troponin I antibody
      • Troponin I cardiac antibody
      • Troponin I cardiac muscle antibody
      • Troponin I cardiac muscle isoform antibody
      • Troponin I type 3 cardiac antibody
      • troponin I, cardiac 3 antibody
      • TroponinI antibody
      • Ttroponin I type 3 (cardiac) antibody
      see all

    Images

    • Flow Cytometry - Alexa Fluor® 488 Anti-Cardiac Troponin I antibody [EP1106Y] (ab196384)
      Flow Cytometry - Alexa Fluor® 488 Anti-Cardiac Troponin I antibody [EP1106Y] (ab196384)
      Overlay histogram showing A673 cells stained with ab196384 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab196384, 1/50 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG [EPR25A] Alexa Fluor® 488 (ab199091) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cardiac Troponin I antibody [EP1106Y] (ab196384)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cardiac Troponin I antibody [EP1106Y] (ab196384)

      ab196384 staining Cardiac Troponin I in A673 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196384 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 2µg/ml (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Alexa Fluor® 488 Anti-Cardiac Troponin I antibody [EP1106Y] (ab196384)
      Alexa Fluor® 488 Anti-Cardiac Troponin I antibody [EP1106Y] (ab196384)

    References (2)

    Publishing research using ab196384? Please let us know so that we can cite the reference in this datasheet.

    ab196384 has been referenced in 2 publications.

    • Karimzadeh F & Opas M Calreticulin Is Required for TGF-ß-Induced Epithelial-to-Mesenchymal Transition during Cardiogenesis in Mouse Embryonic Stem Cells. Stem Cell Reports 8:1299-1311 (2017). Flow Cyt ; Mouse . PubMed: 28434939
    • Cui C  et al. Structural and electrophysiological dysfunctions due to increased endoplasmic reticulum stress in a long-term pacing model using human induced pluripotent stem cell-derived ventricular cardiomyocytes. Stem Cell Res Ther 8:109 (2017). PubMed: 28490375

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