Recombinant Alexa Fluor® 488 Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab185041)
![Flow Cytometry - Alexa Fluor® 488 Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab185041)](https://www.abcam.com/ps/products/185/ab185041/Images/ab185041-250603-anti-catalase-antibody-ep1929y-peroxisome-marker-alexa-fluor-488-flow-cytometry.jpg "Flow Cytometry - Alexa Fluor® 488 Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab185041)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 488 Rabbit monoclonal [EP1929Y] to Catalase - Peroxisome Marker
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Overview
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Product name
Alexa Fluor® 488 Anti-Catalase antibody [EP1929Y] - Peroxisome Marker
See all Catalase primary antibodies -
Description
Alexa Fluor® 488 Rabbit monoclonal [EP1929Y] to Catalase - Peroxisome Marker -
Host species
Rabbit -
Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide within Human Catalase aa 400-500 (C terminal). The exact sequence is proprietary.
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Positive control
- Flow Cyt: HeLa cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EP1929Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab185041 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/100. |
Target
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Function
Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells. -
Involvement in disease
Defects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions. -
Sequence similarities
Belongs to the catalase family. -
Post-translational
modificationsThe N-terminus is blocked. -
Cellular localization
Peroxisome. - Information by UniProt
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Database links
- Entrez Gene: 847 Human
- Entrez Gene: 12359 Mouse
- Omim: 115500 Human
- SwissProt: P04040 Human
- SwissProt: P24270 Mouse
- Unigene: 502302 Human
- Unigene: 4215 Mouse
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Alternative names
- Cas1 antibody
- CAT antibody
- CATA_HUMAN antibody
see all
Images
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Flow Cytometry - Alexa Fluor® 488 Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab185041)
Overlay histogram showing HeLa cells stained with ab185041 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab185041, 1/100 dilution) for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 (ab199091) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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![Alexa Fluor® 488 Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab185041)](https://www.abcam.com/ps/products/185/ab185041/Images/ab185041-2-benefits-of-recombinant-antibodies.png)
Alexa Fluor® 488 Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab185041)
Datasheets and documents
Certificate of Compliance
References (2)
ab185041 has been referenced in 2 publications.
- Kemp K et al. Mesenchymal stem cells restore frataxin expression and increase hydrogen peroxide scavenging enzymes in friedreich ataxia fibroblasts. PLoS One 6:e26098 (2011). WB ; Human . PubMed: 22016819
- Ma HP Hydrogen peroxide stimulates the epithelial sodium channel through a phosphatidylinositide 3-kinase-dependent pathway. J Biol Chem 286:32444-53 (2011). WB ; Xenopus laevis . PubMed: 21795700
