Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 488 Rabbit monoclonal [Y59] to CCR7
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Product nameAlexa Fluor® 488 Anti-CCR7 antibody [Y59]
See all CCR7 primary antibodies
DescriptionAlexa Fluor® 488 Rabbit monoclonal [Y59] to CCR7
ConjugationAlexa Fluor® 488. Ex: 495nm, Em: 519nm
Tested Applications & Species
Application Species ICC/IFHuman
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
- ICC/IF: Jurkat cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or email@example.com.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Concentration information loading...
PurityProtein A purified
- HRP Anti-CCR7 antibody [Y59] (ab196475)
- Alexa Fluor® 647 Anti-CCR7 antibody [Y59] (ab196640)
- Alexa Fluor® 594 Anti-CCR7 antibody [Y59] (ab207016)
- Alexa Fluor® 555 Anti-CCR7 antibody [Y59] (ab207018)
- Alexa Fluor® 568 Anti-CCR7 antibody [Y59] (ab207019)
- Anti-CCR7 antibody [Y59] - Low endotoxin, Azide free (ab221209)
- Anti-CCR7 antibody [Y59] (ab32527)
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab196459 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
FunctionReceptor for the MIP-3-beta chemokine. Probable mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions.
Tissue specificityExpressed in various lymphoid tissues and activated B- and T-lymphocytes, strongly up-regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Cellular localizationCell membrane.
- Information by UniProt
- BLR 2 antibody
- BLR2 antibody
- C C chemokine receptor type 7 antibody
ab196459 staining CCR7 in Jurkat cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab196459 at 1/50 dilution (shown in green) overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
ab196459 has been referenced in 1 publication.
- Gao Z et al. Apatinib enhanced anti-tumor activity of cisplatin on triple-negative breast cancer through inhibition of VEGFR-2. Pathol Res Pract 215:152422 (2019). PubMed: 31079851