RecombinantRabMAb

Recombinant Alexa Fluor® 488 Anti-CD163 antibody [EPR14643-36] - C-terminal (ab218293)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CD163 antibody [EPR14643-36] - C-terminal (ab218293)
  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-CD163 antibody [EPR14643-36] - C-terminal (ab218293)
  • Alexa Fluor® 488 Anti-CD163 antibody [EPR14643-36] - C-terminal (ab218293)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [EPR14643-36] to CD163 - C-terminal
  • Suitable for: IHC-Fr, ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Overview

  • Product name

    Alexa Fluor® 488 Anti-CD163 antibody [EPR14643-36] - C-terminal
    See all CD163 primary antibodies

  • Description

    Alexa Fluor® 488 Rabbit monoclonal [EPR14643-36] to CD163 - C-terminal

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

  • Tested applications

    Suitable for: IHC-Fr, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human CD163 aa 1000 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q86VB7

  • Positive control

    • ICC/IF: Ramos cells. IHC-Fr: normal human liver tissue sections

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab218293 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/100.

This product gave a positive signal in frozen human liver tissue fixed with 10% formaldehyde (10 min).
ICC/IF 1/100.

This product gave a positive signal in Ramos cells fixed with 4% formaldehyde (10 min) and 80% methanol (5 min)

Target

  • Function

    Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.
    After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions.

  • Tissue specificity

    Expressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen, cortical macrophages in the thymus, resident bone marrow macrophages and meningeal macrophages of the central nervous system. Expressed also in blood. Isoform 1 is the lowest abundant in the blood. Isoform 2 is the lowest abundant in the liver and the spleen. Isoform 3 is the predominant isoform detected in the blood.

  • Sequence similarities

    Contains 9 SRCR domains.

  • Domain

    The SRCR domain 3 mediates calcium-sensitive interaction with hemoglobin/haptoglobin complexes.

  • Post-translational
    modifications

    A soluble form (sCD163) is produced by proteolytic shedding which can be induced by lipopolysaccharide, phorbol ester and Fc region of immunoglobulin gamma. This cleavage is dependent on protein kinase C and tyrosine kinases and can be blocked by protease inhibitors. The shedding is inhibited by the tissue inhibitor of metalloproteinase TIMP3, and thus probably induced by membrane-bound metalloproteinases ADAMs.
    Phosphorylated.

  • Cellular localization

    Secreted and Cell membrane. Isoform 1 and isoform 2 show a lower surface expression when expressed in cells.
  • Information by UniProt

  • Database links

    • Alternative names

      • C163A_HUMAN antibody
      • CD 163 antibody
      • CD163 antibody
      • CD163 antigen antibody
      • CD163 molecule antibody
      • Hemoglobin scavenger receptor antibody
      • M130 antibody
      • M130 antigen precursor antibody
      • Macrophage associated antigen antibody
      • MM130 antibody
      • OTTHUMP00000238617 antibody
      • OTTHUMP00000238618 antibody
      • OTTHUMP00000238619 antibody
      • OTTHUMP00000238620 antibody
      • SCARI1 antibody
      • Scavenger receptor cysteine rich type 1 protein M130 antibody
      • sCD163 antibody
      • Soluble CD163 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CD163 antibody [EPR14643-36] - C-terminal (ab218293)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CD163 antibody [EPR14643-36] - C-terminal (ab218293)

      ab218293 staining CD163 in Ramos cells. The cells were fixed with 80% methanol (5 min) and then incubated in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated overnight at +4°C with ab218293 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      This product also gave a positive signal under the same testing conditions in Ramos cells fixed with 4% formaldehyde (10 min).

    • Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-CD163 antibody [EPR14643-36] - C-terminal (ab218293)
      Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-CD163 antibody [EPR14643-36] - C-terminal (ab218293)

      IHC image of CD163 staining in a section of frozen normal human liver.

      The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature.  The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab218293 at 1/100 dilution (shown in green) and counterstained using ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

    • Alexa Fluor® 488 Anti-CD163 antibody [EPR14643-36] - C-terminal (ab218293)
      Alexa Fluor® 488 Anti-CD163 antibody [EPR14643-36] - C-terminal (ab218293)

    References (2)

    Publishing research using ab218293? Please let us know so that we can cite the reference in this datasheet.

    ab218293 has been referenced in 2 publications.

    • Rashid R  et al. Highly multiplexed immunofluorescence images and single-cell data of immune markers in tonsil and lung cancer. Sci Data 6:323 (2019). beta actin ; Human . PubMed: 31848351
    • Doster RS  et al. Streptococcus agalactiae Induces Placental Macrophages To Release Extracellular Traps Loaded with Tissue Remodeling Enzymes via an Oxidative Burst-Dependent Mechanism. MBio 9:N/A (2018). PubMed: 30459195

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