Key features and details
- Alexa Fluor® 488 Rat monoclonal  to CD16+CD32
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Mouse
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
- Isotype: IgG2a
Product nameAlexa Fluor® 488 Anti-CD16+CD32 antibody 
See all CD16+CD32 primary antibodies
DescriptionAlexa Fluor® 488 Rat monoclonal  to CD16+CD32
ConjugationAlexa Fluor® 488. Ex: 495nm, Em: 519nm
Tested applicationsSuitable for: ICC/IF, Flow Cytmore details
Species reactivityReacts with: Mouse
Tissue, cells or virus corresponding to CD16+CD32. Mouse B cells
EpitopeReacts with a conformational epitope formed by CD16 Fc gamma II and CD32 Fc gamma III receptors.
- ICC/IF: RAW 264.7 cells. Flow Cytometry: C57 BL/6 mouse splenocytes.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or firstname.lastname@example.org.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 30% Glycerol
Concentration information loading...
PurityProtein G purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab256417 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product gave a positive signal in Raw264.7 fixed with 100% methanol (5 min) and 4% formaldehyde (10 min).
RelevanceFunction: Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens. Tissue specificity: Found on monocytes, neutrophils and eosinophil platelets. Similarity: Contains 2 Ig-like C2-type (immunoglobulin-like) domains. PTM: Phosphorylated by SRC-type Tyr-kinases such as LYN, BLK, FYN, HCK and SYK.
Cellular localizationCD32: Type I membrane protein. CD16: Attached to the membrane by a GPI anchor. Exists also as a soluble receptor, produced by a proteolytic cleavage.
- CD16a antibody
- CD16b antibody
- CD32 antibody
Flow cytometry staining of C57 BL/6 mouse splenocytes stained with ab256417 (right) or Rat IgG2aκ Alexa Fluor® 488 isotype (left). Cells were incubated for 30 min on ice in 1x PBS containing 10 % mouse serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab256417) or Rat IgG2aκ Alexa Fluor® 488 isotype (1x 106 in 100 µl at 0.2 µg/ml (1/500)) for 30 min on ice. The cells were simultaneously stained with CD19.
Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on live single cells.
ab256417 staining CD16+CD32 in Raw264.7 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab256417 at 1/500 dilution (shown in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in Raw264.7 cells fixed with 4% formaldehyde (10 min).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab256417 has not yet been referenced specifically in any publications.