Recombinant Alexa Fluor® 488 Anti-CD4 antibody [EPR20122] (ab252149)
![Flow Cytometry - Alexa Fluor® 488 Anti-CD4 antibody [EPR20122] (ab252149)](https://www.abcam.com/ps/products/252/ab252149/Images/ab252149-448701-RabbitmonoclonalEPR20122CD4A488MouseC57BL6mousesplenocytescellsflowcytometry.jpg "Flow Cytometry - Alexa Fluor® 488 Anti-CD4 antibody [EPR20122] (ab252149)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 488 Rabbit monoclonal [EPR20122] to CD4
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Overview
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Product name
Alexa Fluor® 488 Anti-CD4 antibody [EPR20122]
See all CD4 primary antibodies -
Description
Alexa Fluor® 488 Rabbit monoclonal [EPR20122] to CD4 -
Host species
Rabbit -
Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment within Mouse CD4 aa 1-400. The exact sequence is proprietary.
Database link: P06332 -
Positive control
- Flow cyt: C57 BL/6 mouse splenocytes.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20122 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab252149 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/100. |
Target
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Function
Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. -
Sequence similarities
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsPalmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts. -
Cellular localization
Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 12504 Mouse
- SwissProt: P06332 Mouse
- Unigene: 2209 Mouse
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Alternative names
- CD 4 antibody
- CD4 (L3T4) antibody
- CD4 antibody
see all
Images
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Flow Cytometry - Alexa Fluor® 488 Anti-CD4 antibody [EPR20122] (ab252149)
Flow cytometry staining of C57 BL/6 mouse splenocytes with ab252149 (right) or Rabbit IgG (monoclonal) Alexa Fluor 488® (ab199091) isotype (left). Cells were incubated for 30 min on ice in 1x PBS containing 10% mouse serum and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab252149) or Rabbit IgG (monoclonal) Alexa Fluor 488® (ab199091) isotype (1x 106 in 100µl at 5µg/ml (1/100)) for 30 min on ice. The cells were simultaneously stained with CD3.
Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.
Events were gated on live lymphocytes.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab252149 has not yet been referenced specifically in any publications.
