Key features and details
- Alexa Fluor® 488 Mouse monoclonal [MEM28] to CD45, prediluted
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
- Isotype: IgG1
Product nameAlexa Fluor® 488 Anti-CD45 antibody [MEM28], prediluted
See all CD45 primary antibodies
DescriptionAlexa Fluor® 488 Mouse monoclonal [MEM28] to CD45, prediluted
ConjugationAlexa Fluor® 488. Ex: 495nm, Em: 519nm
Specificityab187577 reacts with all alternative forms of Human CD45.
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Human
Tissue, cells or virus corresponding to Human CD45. Human thymocytes and T lymphocytes.
Database link: P08575
- Flow Cyt: Human blood.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark.
Storage bufferpH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 99% PBS, 0.2% BSA
Concentration information loading...
Purification notesab187577 is conjugated with Alexa Fluor® 488 under optimum conditions and purified by size-exclusion chromatography and adjusted for direct use.
Our Abpromise guarantee covers the use of ab187577 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
4 μl reagent / 100 μl of whole blood or 106 cells in a suspension.
FunctionProtein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.
Involvement in diseaseDefects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.
Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 tyrosine-protein phosphatase domains.
DomainThe first PTPase domain interacts with SKAP1.
modificationsHeavily N- and O-glycosylated.
Cellular localizationMembrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
- Information by UniProt
- B220 antibody
- CD 45 antibody
- CD45 antibody
Flow cytometry analysis of human peripheral blood labeling CD177 using ab1187590 at 4 µL/100 µL whole blood. Surface staining.
Flow cytometry analysis of human peripheral blood labeling CD45 using ab187577 at 4 µL/100 µL whole blood. CD45-positive lymphocytes (Red) are separated from CD45-negative blood debris(Black, dashed line). Surface staining.
ab187577 has been referenced in 1 publication.
- Tao L et al. Postoperative metastasis prediction based on portal vein circulating tumor cells detected by flow cytometry in periampullary or pancreatic cancer. Cancer Manag Res 11:7405-7425 (2019). PubMed: 31496801