Alexa Fluor® 488 Anti-CD45 antibody [MEM28], prediluted (ab187577)
![Flow Cytometry - Alexa Fluor® 488 Anti-CD45 antibody [MEM28], prediluted (ab187577)](https://www.abcam.com/ps/products/187/ab187577/Images/ab187577-1-anti-cd45-antibody-mem28-flow-cytometry-peripheral-blood-human.jpg "Flow Cytometry - Alexa Fluor® 488 Anti-CD45 antibody [MEM28], prediluted (ab187577)")
Key features and details
- Alexa Fluor® 488 Mouse monoclonal [MEM28] to CD45, prediluted
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
- Isotype: IgG1
Overview
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Product name
Alexa Fluor® 488 Anti-CD45 antibody [MEM28], prediluted
See all CD45 primary antibodies -
Description
Alexa Fluor® 488 Mouse monoclonal [MEM28] to CD45, prediluted -
Host species
Mouse -
Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
Specificity
ab187577 reacts with all alternative forms of Human CD45. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human CD45. Human thymocytes and T lymphocytes.
Database link: P08575 -
Positive control
- Flow Cyt: Human blood.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 99% PBS, 0.2% BSA -
Concentration information loading... -
Purity
Size exclusion -
Purification notes
ab187577 is conjugated with Alexa Fluor® 488 under optimum conditions and purified by size-exclusion chromatography and adjusted for direct use. -
Clonality
Monoclonal -
Clone number
MEM28 -
Isotype
IgG1 -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab187577 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. |
Target
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Function
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. -
Involvement in disease
Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain. -
Sequence similarities
Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 tyrosine-protein phosphatase domains. -
Domain
The first PTPase domain interacts with SKAP1. -
Post-translational
modificationsHeavily N- and O-glycosylated. -
Cellular localization
Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts. - Information by UniProt
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Database links
- Entrez Gene: 5788 Human
- Omim: 151460 Human
- SwissProt: P08575 Human
- Unigene: 654514 Human
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Alternative names
- B220 antibody
- CD 45 antibody
- CD45 antibody
see all
Images
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Flow Cytometry - Alexa Fluor® 488 Anti-CD45 antibody [MEM28], prediluted (ab187577)Flow cytometry analysis of human peripheral blood labeling CD177 using ab1187590 at 4 µL/100 µL whole blood. Surface staining.
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![Flow Cytometry - Alexa Fluor® 488 Anti-CD45 antibody [MEM28], prediluted (ab187577)](https://www.abcam.com/ps/products/187/ab187577/Images/ab187577-2-anti-cd45-antibody-mem28-flow-cytometry-peripheral-blood-human-histogram.jpg)
Flow Cytometry - Alexa Fluor® 488 Anti-CD45 antibody [MEM28], prediluted (ab187577)Flow cytometry analysis of human peripheral blood labeling CD45 using ab187577 at 4 µL/100 µL whole blood. CD45-positive lymphocytes (Red) are separated from CD45-negative blood debris(Black, dashed line). Surface staining.
References (1)
ab187577 has been referenced in 1 publication.
- Tao L et al. Postoperative metastasis prediction based on portal vein circulating tumor cells detected by flow cytometry in periampullary or pancreatic cancer. Cancer Manag Res 11:7405-7425 (2019). PubMed: 31496801
