Alexa Fluor® 488 Anti-CD45RC antibody [MRC OX-22] (ab256260)

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Flow Cytometry - Alexa Fluor® 488 Anti-CD45RC antibody [MRC OX-22] (ab256260)
  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-CD45RC antibody [MRC OX-22] (ab256260)

Key features and details

  • Alexa Fluor® 488 Mouse monoclonal [MRC OX-22] to CD45RC
  • Suitable for: Flow Cyt, IHC-Fr
  • Reacts with: Rat, Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Isotype: IgG1

Overview

  • Product name

    Alexa Fluor® 488 Anti-CD45RC antibody [MRC OX-22]
    See all CD45RC primary antibodies

  • Description

    Alexa Fluor® 488 Mouse monoclonal [MRC OX-22] to CD45RC

  • Host species

    Mouse

  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

  • Tested applications

    Suitable for: Flow Cyt, IHC-Frmore details

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Tissue, cells or virus corresponding to Rat CD45RC. PHA stimulated rat lymphocytes.

  • Positive control

    • Flow Cyt: Lewis rat splenocytes.IHC-Fr: Normal rat spleen tissue sections.

  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab256260 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
1/2500.
IHC-Fr
1/50.
Notes
Flow Cyt
1/2500.
IHC-Fr
1/50.

Target

  • Relevance

    The CD45 family of cell surface glycoprotein antigens exist as a set of isoforms. The patterns of monoclonal antibody recognition of the CD45 phenotypes can be restricted (CD45RA, RB and RC), or broader, such as the CD45 T 200 species. The "restricted" subsets of this group arise as a result of translation of alternatively spliced mRNAs from exons A, B and C (exons 4, 5 and 6), where exons 3-15 encode the extracellular domain of the CD45 molecule. The restricted epitopes are differentially expressed on T and B lymphocytes, including functionally distinct T cells. CD45 (LCA) is a transmembrane phosphotyrosine phosphatase expressed on leucocytes. Myeloid cells do not express the RC isoform.

  • Cellular localization

    Membrane; Single-pass type I membrane protein. Membrane raft. Note: Colocalized with DPP4 in membrane rafts.

  • Database links

    • Alternative names

      • B220 antibody
      • CD45 antibody
      • CD45R antibody
      • EC 3.1.3.48 antibody
      • GP180 antibody
      • L-CA antibody
      • LCA antibody
      • Leukocyte common antigen antibody
      • loc antibody
      • Ly-5 antibody
      • LY5 antibody
      • Ly5, homolog of antibody
      • Lymphocyte antigen 5 antibody
      • Lyt-4 antibody
      • Protein tyrosine phosphatase receptor type C antibody
      • PTPRC antibody
      • Receptor-type tyrosine-protein phosphatase C antibody
      • RT7 antibody
      • T200 antibody
      • T200 Glycoprotein antibody
      see all

    Images

    • Flow Cytometry - Alexa Fluor® 488 Anti-CD45RC antibody [MRC OX-22] (ab256260)
      Flow Cytometry - Alexa Fluor® 488 Anti-CD45RC antibody [MRC OX-22] (ab256260)

      Lewis rat splenocytes stained with ab256260 (right) or mouse IgG1κ Alexa Fluor® 488 isotype (left). Lewis rat splenocytes were incubated for 30 min on ice in 1x PBS / 10 % rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab256260) or mouse IgG1κ Alexa Fluor® 488 isotype (1x 106 in 100 µl at 0.2 µg/ml (1/2500)) for 30 min on ice. The cells were simultaneously stained with CD3.

      Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.

    • Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-CD45RC antibody [MRC OX-22] (ab256260)
      Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-CD45RC antibody [MRC OX-22] (ab256260)

      IHC image of CD45RC staining in a section of frozen normal rat spleen.

      The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature.  The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab256260 at 1/50 dilution (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab256260? Please let us know so that we can cite the reference in this datasheet.

    ab256260 has not yet been referenced specifically in any publications.

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