Alexa Fluor® 488 Anti-CD68 antibody [FA-11] (ab201844)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CD68 antibody [FA-11] (ab201844)

    Key features and details

    • Alexa Fluor® 488 Rat monoclonal [FA-11] to CD68
    • Suitable for: ICC/IF
    • Reacts with: Mouse
    • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
    • Isotype: IgG2a

    Overview

    • Product name

      Alexa Fluor® 488 Anti-CD68 antibody [FA-11]
      See all CD68 primary antibodies

    • Description

      Alexa Fluor® 488 Rat monoclonal [FA-11] to CD68

    • Host species

      Rat

    • Conjugation

      Alexa Fluor® 488. Ex: 495nm, Em: 519nm

    • Tested applications

      Suitable for: ICC/IFmore details

    • Species reactivity

      Reacts with: Mouse

    • Immunogen

      Tissue, cells or virus corresponding to Mouse CD68.

    • Positive control

      • ICC/IF: RAW246.7 cells

    • General notes

      Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.

    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: PBS, 30% Glycerol, 1% BSA
    • Concentration information loading...

    • Purity

      Affinity purified

    • Clonality

      Monoclonal

    • Clone number

      FA-11

    • Isotype

      IgG2a

    • Research areas

    Applications

    Our Abpromise guarantee covers the use of ab201844 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF 1/250.

    This product gave a positive signal in RAW264.7 cells fixed with 100% methanol (5 min)

    Target

    • Function

      Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.

    • Tissue specificity

      Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.

    • Sequence similarities

      Belongs to the LAMP family.

    • Post-translational
      modifications

      N- and O-glycosylated.

    • Cellular localization

      Cell membrane and Endosome membrane. Lysosome membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • CD 68 antibody
        • CD68 antibody
        • CD68 antigen antibody
        • CD68 molecule antibody
        • CD68_HUMAN antibody
        • DKFZp686M18236 antibody
        • gp11 antibody
        • Gp110 antibody
        • LAMP4 antibody
        • Macrophage antigen CD68 (microsialin) antibody
        • MACROPHAGE ANTIGEN CD68 antibody
        • Macrosialin antibody
        • SCARD1 antibody
        • Scavenger receptor class D member 1 antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CD68 antibody [FA-11] (ab201844)
        Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CD68 antibody [FA-11] (ab201844)

        ab201844 staining CD68 in RAW264.7 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab201844 at 1/200 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

         

        Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      References (1)

      Publishing research using ab201844? Please let us know so that we can cite the reference in this datasheet.

      ab201844 has been referenced in 1 publication.

      • Yang J  et al. The influence of PM2.5 on lung injury and cytokines in mice. Exp Ther Med 18:2503-2511 (2019). PubMed: 31572502

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