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    alexa-fluor-488-cd8-alpha-antibody-ep1150y-ab196462.pdf

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RecombinantRabMAb

Recombinant Alexa Fluor® 488 Anti-CD8 alpha antibody [EP1150Y] (ab196462)

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  • SDS
  • Certificate of Compliance
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CD8 alpha antibody [EP1150Y] (ab196462)
  • Alexa Fluor® 488 Anti-CD8 alpha antibody [EP1150Y] (ab196462)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [EP1150Y] to CD8 alpha
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 Carrier Free Unconjugated

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Overview

  • Product name

    Alexa Fluor® 488 Anti-CD8 alpha antibody [EP1150Y]
    See all CD8 alpha primary antibodies
  • Description

    Alexa Fluor® 488 Rabbit monoclonal [EP1150Y] to CD8 alpha
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: Jurkat cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1150Y
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • T Cells
    • Cytotoxic Cells
    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Cancer
    • Tumor immunology
    • CD markers
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Dendritic Cell Lineage
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells

Associated products

  • Alternative Versions

    • Alexa Fluor® 647 Anti-CD8 alpha antibody [EP1150Y] (ab196193)
    • Anti-CD8 alpha antibody [EP1150Y] - Low endotoxin, Azide free (ab210067)
    • Anti-CD8 alpha antibody [EP1150Y] (ab93278)
  • Isotype control

    • Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab196462 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/50.
Notes
ICC/IF
1/50.

Target

  • Function

    Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
  • Involvement in disease

    Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
  • Sequence similarities

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications

    All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
  • Cellular localization

    Secreted and Cell membrane.
  • Target information above from: UniProt accession P01732 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 925 Human
    • Omim: 186910 Human
    • SwissProt: P01732 Human
    • Unigene: 85258 Human
    • Alternative names

      • alpha polypeptide (p32) antibody
      • CD_antigen=CD8a antibody
      • CD8 antibody
      • CD8 antigen alpha polypeptide antibody
      • CD8 antigen alpha polypeptide (p32) antibody
      • CD8 antigen, alpha polypeptide (p32) antibody
      • CD8a antibody
      • CD8A antigen antibody
      • CD8A molecule antibody
      • CD8A_HUMAN antibody
      • Leu2 antibody
      • Leu2 T lymphocyte antigen antibody
      • Ly 2 antibody
      • Ly 35 antibody
      • Ly B antibody
      • Ly2 antibody
      • Ly3 antibody
      • Ly35 antibody
      • LyB antibody
      • Lyt 2.1 lymphocyte differentiation antigen (AA at 100) antibody
      • LYT3 antibody
      • MAL antibody
      • OKT8 T cell antigen antibody
      • OTTHUMP00000160760 antibody
      • OTTHUMP00000160764 antibody
      • OTTHUMP00000203528 antibody
      • OTTHUMP00000203721 antibody
      • p32 antibody
      • T cell antigen Leu2 antibody
      • T cell co receptor antibody
      • T lymphocyte differentiation antigen T8/Leu 2 antibody
      • T-cell surface glycoprotein CD8 alpha chain antibody
      • T-cell surface glycoprotein Lyt 2 antibody
      • T-lymphocyte differentiation antigen T8/Leu-2 antibody
      • T8 T cell antigen antibody
      • T8/Leu-2 T-lymphocyte differentiation antigen antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CD8 alpha antibody [EP1150Y] (ab196462)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CD8 alpha antibody [EP1150Y] (ab196462)

      ab196462 staining CD8 alpha in Jurkat cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab196462 at 1/50 dilution (shown in green) overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Alexa Fluor® 488 Anti-CD8 alpha antibody [EP1150Y] (ab196462)
      Alexa Fluor® 488 Anti-CD8 alpha antibody [EP1150Y] (ab196462)

    Protocols

    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab196462? Please let us know so that we can cite the reference in this datasheet.

    ab196462 has not yet been referenced specifically in any publications.

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