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  1. Link

    alexa-fluor-488-cdkn2ap16ink4a-antibody-epr1473-ab192053.pdf

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RecombinantRabMAb

Recombinant Alexa Fluor® 488 Anti-CDKN2A/p16INK4a antibody [EPR1473] (ab192053)

  • Datasheet
  • Certificate of Compliance
Reviews (1) Submit a question References (1)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CDKN2A/p16INK4a antibody [EPR1473] (ab192053)
  • Alexa Fluor® 488 Anti-CDKN2A/p16INK4a antibody [EPR1473] (ab192053)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [EPR1473] to CDKN2A/p16INK4a
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

You may also be interested in

Primary
Product image
Alexa Fluor® 647 Anti-CDKN2A/p16INK4a antibody [EPR1473] (ab192054)
Protein
Product image
Recombinant Human CDKN2A/p16INK4a protein (ab84075)

View more associated products

Overview

  • Product name

    Alexa Fluor® 488 Anti-CDKN2A/p16INK4a antibody [EPR1473]
    See all CDKN2A/p16INK4a primary antibodies
  • Description

    Alexa Fluor® 488 Rabbit monoclonal [EPR1473] to CDKN2A/p16INK4a
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CDKN2A/p16INK4a aa 100 to the C-terminus (C terminal). The exact sequence is proprietary.

  • Positive control

    • ICC/IF: HeLa cells.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 30% Glycerol, PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR1473
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • Ink4
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cell Cycle Inhibitors
    • Ink4
    • Cancer
    • Cell cycle
    • Cell cycle inhibitors
    • Ink4
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • INK4a/Arf

Associated products

  • Alternative Versions

    • Anti-CDKN2A/p16INK4a antibody [EPR1473] - C-terminal (ab108349)
    • Anti-CDKN2A/p16INK4a antibody [EPR1473] - BSA and Azide free (ab186932)
    • Alexa Fluor® 647 Anti-CDKN2A/p16INK4a antibody [EPR1473] (ab192054)
    • HRP Anti-CDKN2A/p16INK4a antibody [EPR1473] (ab192080)
    • PE Anti-CDKN2A/p16INK4a antibody [EPR1473] (ab209579)
  • Isotype control

    • Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)
  • Positive Controls

    • HeLa nuclear extract lysate (ab14655)
    • Saos 2 nuclear extract lysate (ab14891)
  • Recombinant Protein

    • Recombinant Human CDKN2A/p16INK4a protein (ab84075)

Applications

Our Abpromise guarantee covers the use of ab192053 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50.

Target

  • Cellular localization

    Cytoplasmic and Nuclear
  • Database links

    • Entrez Gene: 1029 Human
    • Omim: 600160 Human
    • SwissProt: P42771 Human
    • Unigene: 512599 Human
    • Form

      There are 4 isoforms produced by alternative splicing. Isoform 1 also known as: p16INK4a; Isoform 3 also known as: p12; Isoform 4 also known as: p14ARF; p19ARF; ARF.
    • Alternative names

      • CCM2 antibody
      • CDK4 inhibitor p16 INK4 antibody
      • CDK4I antibody
      • CDKN2 antibody
      • CDKN2A antibody
      • Cell cycle negative regulator beta antibody
      • CMM2 antibody
      • Cyclin dependent kinase 4 inhibitor A antibody
      • Cyclin dependent kinase inhibitor 2A (melanoma p16 inhibits CDK4) antibody
      • Cyclin Dependent Kinase Inhibitor 2A antibody
      • Cyclin dependent kinase inhibitor 2A isoform 4 antibody
      • Cyclin dependent kinase inhibitor 2A isoforms 1/2/3 antibody
      • Cyclin dependent kinase inhibitor p16 antibody
      • INK4 antibody
      • INK4A antibody
      • MLM antibody
      • MTS1 antibody
      • Multiple tumor suppressor 1 antibody
      • p14 antibody
      • p16 antibody
      • P16INK4 antibody
      • p16INK4a antibody
      • p19 antibody
      • p19Arf antibody
      • TP16 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CDKN2A/p16INK4a antibody [EPR1473] (ab192053)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CDKN2A/p16INK4a antibody [EPR1473] (ab192053)

      ab192053 staining CDKN2A/p16INK4a in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab192053 at a working dilution of 1 in 50 (shown in green) and ab195889, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 594, shown in red) at 2µg/ml overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Alexa Fluor® 488 Anti-CDKN2A/p16INK4a antibody [EPR1473] (ab192053)
      Alexa Fluor® 488 Anti-CDKN2A/p16INK4a antibody [EPR1473] (ab192053)

    Protocols

    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (1)

    Publishing research using ab192053? Please let us know so that we can cite the reference in this datasheet.

    ab192053 has been referenced in 1 publication.

    • Bacqueville D  et al. Efficacy of a Dermocosmetic Serum Combining Bakuchiol and Vanilla Tahitensis Extract to Prevent Skin Photoaging in vitro and to Improve Clinical Outcomes for Naturally Aged Skin. Clin Cosmet Investig Dermatol 13:359-370 (2020). PubMed: 32494181

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Flow Cytometry abreview for Anti-CDKN2A/p16INK4a antibody [EPR1473] (Alexa Fluor® 488)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (kidney embryonic cell lines)
    Permeabilization
    Yes - 88% Methanol
    Gating Strategy
    live cells
    Specification
    kidney embryonic cell lines
    Preparation
    Cell harvesting/tissue preparation method: trypsin
    Sample buffer: PBS
    Fixation
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 06 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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