RecombinantRabMAb

Recombinant Alexa Fluor® 488 Anti-CDX2 antibody [EPR2764Y] (ab195007)

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Flow Cytometry - Alexa Fluor® 488 Anti-CDX2 antibody [EPR2764Y] (ab195007)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CDX2 antibody [EPR2764Y] (ab195007)
  • Alexa Fluor® 488 Anti-CDX2 antibody [EPR2764Y] (ab195007)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [EPR2764Y] to CDX2
  • Suitable for: Flow Cyt, ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Overview

  • Product name

    Alexa Fluor® 488 Anti-CDX2 antibody [EPR2764Y]
    See all CDX2 primary antibodies

  • Description

    Alexa Fluor® 488 Rabbit monoclonal [EPR2764Y] to CDX2

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

  • Tested applications

    Suitable for: Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit

  • Immunogen

    Synthetic peptide within Human CDX2 aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • ICC/IF: SW480 cells. Flow Cyt: SW480 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab195007 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50.

ab199091 - Rabbit monoclonal IgG (Alexa Fluor® 488), is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.

Target

  • Function

    Involved in the transcriptional regulation of multiple genes expressed in the intestinal epithelium. Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine.

  • Sequence similarities

    Belongs to the Caudal homeobox family.
    Contains 1 homeobox DNA-binding domain.

  • Post-translational
    modifications

    Phosphorylation of Ser-60 mediates the transactivation capacity.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Caudal type homeo box 2 antibody
    • Caudal type homeo box transcription factor 2 antibody
    • Caudal type homeobox 2 antibody
    • Caudal type homeobox protein 2 antibody
    • Caudal type homeobox transcription factor 2 antibody
    • Caudal-type homeobox protein 2 antibody
    • CDX 2 antibody
    • CDX 3 antibody
    • CDX-3 antibody
    • Cdx2 antibody
    • CDX2/AS antibody
    • CDX2_HUMAN antibody
    • CDX3 antibody
    • Homeobox protein CDX 2 antibody
    • Homeobox protein CDX-2 antibody
    see all

Images

  • Flow Cytometry - Alexa Fluor® 488 Anti-CDX2 antibody [EPR2764Y] (ab195007)
    Flow Cytometry - Alexa Fluor® 488 Anti-CDX2 antibody [EPR2764Y] (ab195007)

    Overlay histogram showing SW480 cells stained with ab195007 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab195007, 1/50 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    This antibody gave a positive signal in SW480 fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CDX2 antibody [EPR2764Y] (ab195007)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CDX2 antibody [EPR2764Y] (ab195007)

    ab195007 staining CDX2 in SW480 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab195007 at a 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Alexa Fluor® 488 Anti-CDX2 antibody [EPR2764Y] (ab195007)
    Alexa Fluor® 488 Anti-CDX2 antibody [EPR2764Y] (ab195007)

References (1)

Publishing research using ab195007? Please let us know so that we can cite the reference in this datasheet.

ab195007 has been referenced in 1 publication.

  • Menheniott TR  et al. Loss of gastrokine-2 drives premalignant gastric inflammation and tumor progression. J Clin Invest 126:1383-400 (2016). PubMed: 26974160

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