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  1. Link

    alexa-fluor-488-cdx2-antibody-epr2764y-ab195007.pdf

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Stem Cells Lineage Markers Endoderm
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RecombinantRabMAb

Recombinant Alexa Fluor® 488 Anti-CDX2 antibody [EPR2764Y] (ab195007)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CDX2 antibody [EPR2764Y] (ab195007)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CDX2 antibody [EPR2764Y] (ab195007)
  • Alexa Fluor® 488 Anti-CDX2 antibody [EPR2764Y] (ab195007)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [EPR2764Y] to CDX2
  • Suitable for: IHC-P
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Conjugates logo Related conjugates and formulations

APC HRP PE PE Unconjugated

You may also be interested in

Protein
Product image
Recombinant Human CDX2 protein (ab114247)
 
Product image
RGD (Arg-Gly-Asp), sequence involved in cell adhesion (ab142698)
Primary
Product image
Alexa Fluor® 647 Anti-CDX2 antibody [EPR2764Y] (ab195008)

View more associated products

Overview

  • Product name

    Alexa Fluor® 488 Anti-CDX2 antibody [EPR2764Y]
    See all CDX2 primary antibodies
  • Description

    Alexa Fluor® 488 Rabbit monoclonal [EPR2764Y] to CDX2
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested applications

    Suitable for: IHC-Pmore details
    Unsuitable for: Flow Cyt (Intra) or ICC/IF
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human normal colon tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Dissociation constant (KD)

    KD = 1.00 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR2764Y
  • Isotype

    IgG
  • Research areas

    • Stem Cells
    • Lineage Markers
    • Endoderm
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Developmental Biology
    • Lineage specification
    • Endoderm
    • Developmental Biology
    • Lineage specification
    • Trophectoderm

Associated products

  • Alternative Versions

    • Alexa Fluor® 647 Anti-CDX2 antibody [EPR2764Y] (ab195008)
    • Anti-CDX2 antibody [EPR2764Y] - BSA and Azide free (ab220799)
    • Anti-CDX2 antibody [EPR2764Y] (ab76541)
  • Isotype control

    • Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)
  • Recombinant Protein

    • Recombinant Human CDX2 protein (ab114247)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab195007 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Application notes
Is unsuitable for Flow Cyt (Intra) or ICC/IF.

Target

  • Function

    Involved in the transcriptional regulation of multiple genes expressed in the intestinal epithelium. Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine.
  • Sequence similarities

    Belongs to the Caudal homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Post-translational
    modifications

    Phosphorylation of Ser-60 mediates the transactivation capacity.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q99626 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1045 Human
    • Entrez Gene: 12591 Mouse
    • Entrez Gene: 66019 Rat
    • Omim: 600297 Human
    • SwissProt: Q99626 Human
    • SwissProt: P43241 Mouse
    • Unigene: 174249 Human
    • Unigene: 20358 Mouse
    • Alternative names

      • Caudal type homeo box 2 antibody
      • Caudal type homeo box transcription factor 2 antibody
      • Caudal type homeobox 2 antibody
      • Caudal type homeobox protein 2 antibody
      • Caudal type homeobox transcription factor 2 antibody
      • Caudal-type homeobox protein 2 antibody
      • CDX 2 antibody
      • CDX 3 antibody
      • CDX-3 antibody
      • Cdx2 antibody
      • CDX2/AS antibody
      • CDX2_HUMAN antibody
      • CDX3 antibody
      • Homeobox protein CDX 2 antibody
      • Homeobox protein CDX-2 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CDX2 antibody [EPR2764Y] (ab195007)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CDX2 antibody [EPR2764Y] (ab195007)

      Immunofluorescence staining of CDX2 in a section of formalin-fixed paraffin-embedded human normal colon.

      Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) using retrieval settings of 110°C for 40 minutes. The section was then incubated at room temperature for 1 hour with ab195007 at 1/100 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CDX2 antibody [EPR2764Y] (ab195007)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CDX2 antibody [EPR2764Y] (ab195007)

      Immunofluorescence showing negative staining of CDX2 in a section of formalin-fixed paraffin-embedded human skeletal muscle. 

      Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) using retrieval settings of 110°C for 40 minutes. The section was then incubated at room temperature for 1 hour with ab195007 at 1/100 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

    • Alexa Fluor® 488 Anti-CDX2 antibody [EPR2764Y] (ab195007)
      Alexa Fluor® 488 Anti-CDX2 antibody [EPR2764Y] (ab195007)

    Protocols

    • Flow cytometry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab195007? Please let us know so that we can cite the reference in this datasheet.

    ab195007 has been referenced in 1 publication.

    • Menheniott TR  et al. Loss of gastrokine-2 drives premalignant gastric inflammation and tumor progression. J Clin Invest 126:1383-400 (2016). PubMed: 26974160

    Customer reviews and Q&As

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