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  1. Link

    alexa-fluor-488-cpt1a-antibody-8f6ae9-ab171449.pdf

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Validated using a knockout cell line

Alexa Fluor® 488 Anti-CPT1A antibody [8F6AE9] (ab171449)

  • Datasheet
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Flow Cytometry - Alexa Fluor® 488 Anti-CPT1A antibody [8F6AE9] (ab171449)
  • Flow Cytometry - Alexa Fluor® 488 Anti-CPT1A antibody [8F6AE9] (ab171449)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CPT1A antibody [8F6AE9] (ab171449)

Key features and details

  • Alexa Fluor® 488 Mouse monoclonal [8F6AE9] to CPT1A
  • Suitable for: ICC/IF, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Rat, Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Isotype: IgG2b

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Conjugation
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Alexa Fluor® 488 Conjugation Kit (Fast) - Lightning-Link® (ab236553)

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Overview

  • Product name

    Alexa Fluor® 488 Anti-CPT1A antibody [8F6AE9]
    See all CPT1A primary antibodies
  • Description

    Alexa Fluor® 488 Mouse monoclonal [8F6AE9] to CPT1A
  • Host species

    Mouse
  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested applications

    Suitable for: ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Does not react with: Cow
  • Immunogen

    Recombinant fragment corresponding to Human CPT1A aa 450 to the C-terminus (C terminal).
    Database link: P50416

  • Positive control

    • Flow cytometry: HeLa cells, HAP1-WT cells. ICC/IF: HeLa cells.
  • General notes

    Product was previously marketed under the MitoSciences sub-brand.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: 1% BSA, 98% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purity is near homogeneity as judged by SDS-PAGE. ab171449 was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by ammonium sulfate precipitation.
  • Clonality

    Monoclonal
  • Clone number

    8F6AE9
  • Isotype

    IgG2b
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Mitochondria
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cardiovascular
    • Lipids / Lipoproteins
    • Fatty Acids
    • Metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Fatty acids
    • Metabolism
    • Types of disease
    • Obesity
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Fatty acid oxidation

Associated products

  • Alternative Versions

    • Anti-CPT1A antibody [8F6AE9] (ab128568)
    • HRP Anti-CPT1A antibody [8F6AE9] (ab198494)
  • Related Products

    • Recombinant Human CPT1A protein (ab128569)

Applications

Our Abpromise guarantee covers the use of ab171449 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
Flow Cyt Use a concentration of 5 µg/ml.

ab171465 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

 

Target

  • Tissue specificity

    Strong expression in kidney and heart, and lower in liver and skeletal muscle.
  • Pathway

    Lipid metabolism; fatty acid beta-oxidation.
  • Involvement in disease

    Defects in CPT1A are the cause of carnitine palmitoyltransferase 1A deficiency (CPT1AD) [MIM:255120]; also known as CPT-I deficiency or CPT1A deficiency. CPT1AD is a rare autosomal recessive metabolic disorder of long-chain fatty acid oxidation characterized by severe episodes of hypoketotic hypoglycemia usually occurring after fasting or illness. Onset is in infancy or early childhood.
  • Sequence similarities

    Belongs to the carnitine/choline acetyltransferase family.
  • Cellular localization

    Mitochondrion outer membrane.
  • Target information above from: UniProt accession P50416 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1374 Human
    • Entrez Gene: 12894 Mouse
    • Entrez Gene: 25757 Rat
    • Omim: 600528 Human
    • SwissProt: P50416 Human
    • SwissProt: P97742 Mouse
    • SwissProt: P32198 Rat
    • Unigene: 503043 Human
    • Unigene: 18522 Mouse
    • Unigene: 2856 Rat
    see all
  • Alternative names

    • Carnitine O palmitoyltransferase 1 liver isoform antibody
    • Carnitine O palmitoyltransferase I antibody
    • Carnitine O palmitoyltransferase I liver isoform antibody
    • Carnitine O-palmitoyltransferase 1 antibody
    • Carnitine O-palmitoyltransferase I antibody
    • Carnitine palmitoyltransferase 1A (liver) antibody
    • Carnitine palmitoyltransferase 1A antibody
    • Carnitine palmitoyltransferase I antibody
    • Carnitine palmitoyltransferase I liver antibody
    • CPT 1 antibody
    • CPT I antibody
    • CPT1 antibody
    • CPT1 L antibody
    • CPT1-L antibody
    • Cpt1a antibody
    • CPT1A_HUMAN antibody
    • CPTI antibody
    • CPTI-L antibody
    • L CPT1 antibody
    • liver isoform antibody
    see all

Images

  • Flow Cytometry - Alexa Fluor® 488 Anti-CPT1A antibody [8F6AE9] (ab171449)
    Flow Cytometry - Alexa Fluor® 488 Anti-CPT1A antibody [8F6AE9] (ab171449)

    Overlay histogram showing HAP1 wildtype (green line) and HAP1-CPT1A  knockout cells (red line) stained with ab171449. The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab171449, 1µg/ml dilution) for 30 min at 22°C.

    A rabbit monoclonal IgG isotype control antibody  (ab199091) was used at the same concentration and conditions as the primary antibody (HAP1 wildtype - black line, HAP1-CPT1A    knockout - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

    Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

  • Flow Cytometry - Alexa Fluor® 488 Anti-CPT1A antibody [8F6AE9] (ab171449)
    Flow Cytometry - Alexa Fluor® 488 Anti-CPT1A antibody [8F6AE9] (ab171449)

    Flow cytometric analysis of HeLa cells (4% paraformaldehyde-fixed;  methanol-permeablized) labeling CPT1A with ab171449 at 5 µg/mL (green) compared with an isotype control ab170192 at 5 µg/mL (black).  Isotype control was labeled with a Goat Anti-mouse Alexa Fluor® 488 secondary antibody prior to signal measurement on the FL-1 channel.

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CPT1A antibody [8F6AE9] (ab171449)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CPT1A antibody [8F6AE9] (ab171449)

    Immunofluoresecence analysis of HeLa cells (4% paraformaldehyde-fixed, methanol-permeablized) labeling CPT1A with ab171449 at 5µg/ml overnight.  100x magnification.

    A) HeLa cells labeled with ab171449 at 5 µg/mL.
    B) HeLa cells labeled with Anti-HSP60 (1/3000, ab46798), Secondary antibody used was goat anti-rabbit Dylight-594 (1/1000, ab96897).
    C) Merged Image of A and B showing specificity of mitochondrial staining.

     

Protocols

  • Flow cytometry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (5)

    Publishing research using ab171449? Please let us know so that we can cite the reference in this datasheet.

    ab171449 has been referenced in 5 publications.

    • Sawyer BT  et al. Targeting Fatty Acid Oxidation to Promote Anoikis and Inhibit Ovarian Cancer Progression. Mol Cancer Res 18:1088-1098 (2020). PubMed: 32198139
    • Kurupati RK  et al. Age-related changes in B cell metabolism. Aging (Albany NY) 11:4367-4381 (2019). Flow Cyt . PubMed: 31283526
    • Felices M  et al. Continuous treatment with IL-15 exhausts human NK cells via a metabolic defect. JCI Insight 3:N/A (2018). PubMed: 29415897
    • Zhang Y  et al. Enhancing CD8+ T Cell Fatty Acid Catabolism within a Metabolically Challenging Tumor Microenvironment Increases the Efficacy of Melanoma Immunotherapy. Cancer Cell 32:377-391.e9 (2017). PubMed: 28898698
    • Nguyen HD  et al. Metabolic reprogramming of alloantigen-activated T cells after hematopoietic cell transplantation. J Clin Invest 126:1337-52 (2016). PubMed: 26950421

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Flow Cytometry abreview for Anti-CPT1A antibody [8F6AE9] (Alexa Fluor® 488)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Mouse Cell (Splenocytes/T cells)
    Permeabilization
    Yes - FoxP3 buffer set (Invitrogen)
    Gating Strategy
    Size gate (lymphocyte), singlets, viable (FVD 506), CD90.1+
    Specification
    Splenocytes/T cells
    Preparation
    Cell harvesting/tissue preparation method: Spleen digestion with Liberase TM and DNAse1
    Sample buffer: PBS with 2 mM EDTA and 1% FBS
    Fixation
    FoxP3 buffer set (Invitrogen)
    Read More

    Dr. Vedran Radojcic

    Verified customer

    Submitted Nov 30 2019

    Flow Cytometry abreview for Anti-CPT1A antibody [8F6AE9] (Alexa Fluor® 488)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (PBMCs)
    Permeabilization
    Yes - Ebioscience kit
    Gating Strategy
    We are evaluating CPT1a in a variety of subsets including T cells and myeloid cells
    Specification
    PBMCs
    Preparation
    Cell harvesting/tissue preparation method: Freshly thawed human PBMCs
    Sample buffer: PBS
    Fixation
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 05 2019

    Flow Cytometry abreview for Anti-CPT1A antibody [8F6AE9] (Alexa Fluor® 488)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (PBMCs)
    Permeabilization
    Yes - BD Cytofix/Cytoperm™
    Gating Strategy
    lymphocytes/single cells/live cells/ CD4+ or CD8+ with subpopulations
    Specification
    PBMCs
    Preparation
    Cell harvesting/tissue preparation method: thawed PBMCs
    Sample buffer: PBS/FACS buffer
    Fixation
    BD Cytofix/Cytoperm™
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 14 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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