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Alexa Fluor® 488 Anti-Cytochrome C antibody [37BA11] (ab154476)

  • Datasheet
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytochrome C antibody [37BA11] (ab154476)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytochrome C antibody [37BA11] (ab154476)
  • Flow Cytometry - Alexa Fluor® 488 Anti-Cytochrome C antibody [37BA11] (ab154476)

Key features and details

  • Alexa Fluor® 488 Mouse monoclonal [37BA11] to Cytochrome C
  • Suitable for: Flow Cyt, ICC/IF
  • Reacts with: Mouse, Rat, Cow, Human, Caenorhabditis elegans
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Isotype: IgG2a

You may also be interested in

Protein
Recombinant Human Cytochrome C protein (ab172817)
Primary
Product image
Alexa Fluor® 647 Anti-Cytochrome C antibody [37BA11] (ab198583)

View more associated products

Overview

  • Product name

    Alexa Fluor® 488 Anti-Cytochrome C antibody [37BA11]
    See all Cytochrome C primary antibodies
  • Description

    Alexa Fluor® 488 Mouse monoclonal [37BA11] to Cytochrome C
  • Host species

    Mouse
  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested applications

    Suitable for: Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Human, Caenorhabditis elegans
  • Immunogen

    Full length native protein (purified) corresponding to Cow Cytochrome C.

  • General notes

    Product was previously marketed under the MitoSciences sub-brand.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: 1% BSA, 30% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab154476 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
  • Clonality

    Monoclonal
  • Clone number

    37BA11
  • Isotype

    IgG2a
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cytochromes
    • Cell Biology
    • Apoptosis
    • Mitochondrial
    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Cytochrome C
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Oxidative phosphorylation
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipases
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Cytochromes
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Cytochrome C
    • Cancer
    • Cell Death
    • Apoptosis
    • Mitochondrial
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Associated products

  • Alternative Versions

    • Anti-Cytochrome C antibody [37BA11] (ab110325)
    • HRP Anti-Cytochrome C antibody [37BA11] (ab198489)
    • Alexa Fluor® 647 Anti-Cytochrome C antibody [37BA11] (ab198583)
  • Isotype control

    • Alexa Fluor® 488 Mouse IgG2a monoclonal [PPV-04] - Isotype Control (ab237608)
  • Recombinant Protein

    • Recombinant Human Cytochrome C protein (ab172817)
  • Related Products

    • Anti-Hsp60 antibody (ab46798)

Applications

Our Abpromise guarantee covers the use of ab154476 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use a concentration of 0.2 µg/ml.

ab171464 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

ICC/IF Use a concentration of 1 µg/ml.

Target

  • Function

    Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
    Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
  • Involvement in disease

    Defects in CYCS are the cause of thrombocytopenia type 4 (THC4) [MIM:612004]; also known as autosomal dominant thrombocytopenia type 4. Thrombocytopenia is the presence of relatively few platelets in blood. THC4 is a non-syndromic form of thrombocytopenia. Clinical manifestations of thrombocytopenia are absent or mild. THC4 may be caused by dysregulated platelet formation.
  • Sequence similarities

    Belongs to the cytochrome c family.
  • Post-translational
    modifications

    Binds 1 heme group per subunit.
  • Cellular localization

    Mitochondrion matrix.
  • Target information above from: UniProt accession P99999 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 510767 Cow
    • Entrez Gene: 54205 Human
    • Entrez Gene: 13063 Mouse
    • Entrez Gene: 25309 Rat
    • Omim: 123970 Human
    • SwissProt: P62894 Cow
    • SwissProt: P99999 Human
    • SwissProt: P62897 Mouse
    • SwissProt: P62898 Rat
    • Unigene: 437060 Human
    • Unigene: 617193 Human
    • Unigene: 35389 Mouse
    • Unigene: 2202 Rat
    see all
  • Alternative names

    • CYC antibody
    • CYC_HUMAN antibody
    • CYCS antibody
    • Cytochrome c antibody
    • Cytochrome c somatic antibody
    • HCS antibody
    • THC4 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytochrome C antibody [37BA11] (ab154476)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytochrome C antibody [37BA11] (ab154476)
    Immunocytochemistry with HeLa cells (100x) were stained with Anti-Cytochrome C Alexa-488 antibody (1.0 µg/mL, ab154476) in green and DAPI in blue, as a nuclear stain.
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytochrome C antibody [37BA11] (ab154476)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytochrome C antibody [37BA11] (ab154476)
    Immunocytochemistry with HDFn (100x) cells were stained with Anti-Cytochrome C Alexa-488 antibody (1.0 µg/mL, ab154476) in green, Anti-HSP60 (1/1000, ab46798) as red, and DAPI in blue, as a nuclear stain. Secondary antibody used was goat anti-rabbit dyelight-594 (1/1000, ab96897).
  • Flow Cytometry - Alexa Fluor® 488 Anti-Cytochrome C antibody [37BA11] (ab154476)
    Flow Cytometry - Alexa Fluor® 488 Anti-Cytochrome C antibody [37BA11] (ab154476)
    Flow Cytometry for Anti-Cytochrome C Alexa-488 antibody (ab154476) stained HeLa cells. Flow cytometry results of unstained cells (black) and 0.2 µg/mL Cytochrome C Alexa-488 (red) stained cells.

Protocols

  • Flow cytometry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (3)

    Publishing research using ab154476? Please let us know so that we can cite the reference in this datasheet.

    ab154476 has been referenced in 3 publications.

    • Zhou H  et al. The oncolytic compound LTX-401 targets the Golgi apparatus. Cell Death Differ 23:2031-2041 (2016). PubMed: 27588704
    • Tian XF  et al. PACE4 regulates apoptosis in human pancreatic cancer Panc-1 cells via the mitochondrial signaling pathway. Mol Med Rep 14:5205-5210 (2016). PubMed: 27779720
    • Zhou H  et al. The oncolytic peptide LTX-315 kills cancer cells through Bax/Bak-regulated mitochondrial membrane permeabilization. Oncotarget 6:26599-614 (2015). PubMed: 26378049

    Customer reviews and Q&As

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