Validated using a knockout cell lineRecombinantRabMAb

Recombinant Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EP1580Y] (ab192643)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EP1580Y] (ab192643)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EP1580Y] (ab192643)
  • Flow Cytometry - Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EP1580Y] (ab192643)
  • Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EP1580Y] (ab192643)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [EP1580Y] to Cytokeratin 19
  • Suitable for: ICC/IF, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Overview

  • Product name

    Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EP1580Y]
    See all Cytokeratin 19 primary antibodies

  • Description

    Alexa Fluor® 488 Rabbit monoclonal [EP1580Y] to Cytokeratin 19

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

  • Tested applications

    Suitable for: ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide within Human Cytokeratin 19 (C terminal). The exact sequence is proprietary.

  • Positive control

    • ICC/IF: HeLa. ICC/IF KO: MCF7 cells (MCF7-KRT19 KO used as a negative cell line). Flow Cyt: HeLa

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.

  • Dissociation constant (KD)

    KD = 3.70 x 10 -10 M
    Learn more about KD

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 30% Glycerol, 1% BSA, PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Monoclonal

  • Clone number

    EP1580Y

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab192643 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.

Signal can be observed in cells fixed with either methanol or paraformaldehyde.
Flow Cyt 1/500.

Target

  • Function

    Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

  • Tissue specificity

    Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin.

  • Sequence similarities

    Belongs to the intermediate filament family.

  • Developmental stage

    Present in hair follicles at all stages of development.

  • Domain

    This keratin differs from all other IF proteins in lacking the C-terminal tail domain.
  • Information by UniProt

  • Database links

    • Alternative names

      • 40 kDa keratin intermediate filament antibody
      • CK 19 antibody
      • CK-19 antibody
      • CK19 antibody
      • Cytokeratin 19 antibody
      • Cytokeratin-19 antibody
      • K19 antibody
      • K1C19_HUMAN antibody
      • K1CS antibody
      • Keratin 19 antibody
      • Keratin type I 40 kD antibody
      • Keratin type I 40kD antibody
      • Keratin type I cytoskeletal 19 antibody
      • Keratin, type I cytoskeletal 19 antibody
      • Keratin, type I, 40 kd antibody
      • Keratin-19 antibody
      • KRT19 antibody
      • MGC15366 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EP1580Y] (ab192643)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EP1580Y] (ab192643)

      ab192643 staining Cytokeratin 19 in wild-type MCF7 cells (top panel) and KRT19 knockout MCF7 cells (bottom panel). The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab192643 at 1/500 dilution (shown in green) and ab195884 (Rat monoclonal to Tubulin - Alexa Fluor® 647) at 1/100 dilution (shown in red) overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.
      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EP1580Y] (ab192643)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EP1580Y] (ab192643)

      ab192643 staining Cytokeratin 19 in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells

      The cells were fixed with 4% formaldehyde (10 min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Triton X-100 for 1hr. The cells were then incubated with ab192643 at a working dilution of 1 in 100 (shown in green) and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin) at 1 µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1hr with an AlexaFluor® 594 Goat anti-mouse IgG (H&L - preadsorbed) secondary (ab150120) at 2 μg/ml (shown in pseudo-color red).

      Nuclear DNA was labeled in blue with DAPI.

      This product gave a positive signal in 100% methanol (5 min) fixed HeLa cells under the same testing conditions.

      Image was taken with a Confocal microscope (Leica-microsystems, TCS SP8)

    • Flow Cytometry - Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EP1580Y] (ab192643)
      Flow Cytometry - Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EP1580Y] (ab192643)

      Overlay histogram showing HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained with ab192643 (red line).

      The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab192643, 1/500 dilution) for 30 min at 22°C.

      Isotype control antibody (black line) was rabbit monoclonal IgG [EPR25A] Alexa Fluor® 488 (ab199091) used at the same concentration and conditions as the primary antibody. Unlabeled sample (blue line) was also used as a control.

      Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    • Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EP1580Y] (ab192643)
      Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EP1580Y] (ab192643)

    References (0)

    Publishing research using ab192643? Please let us know so that we can cite the reference in this datasheet.

    ab192643 has not yet been referenced specifically in any publications.

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