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  1. Link

    alexa-fluor-488-cytokeratin-19-antibody-ep1580y-ab192643.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Intermediate Filaments Class I Cytokeratins
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EP1580Y] (ab192643)

  • Datasheet
  • SDS
  • Certificate of Compliance
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EP1580Y] (ab192643)
  • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EP1580Y] (ab192643)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EP1580Y] (ab192643)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EP1580Y] (ab192643)
  • Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EP1580Y] (ab192643)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [EP1580Y] to Cytokeratin 19
  • Suitable for: Flow Cyt (Intra), ICC/IF
  • Knockout validated
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 APC Carrier Free HRP PE Unconjugated

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Overview

  • Product name

    Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EP1580Y]
    See all Cytokeratin 19 primary antibodies
  • Description

    Alexa Fluor® 488 Rabbit monoclonal [EP1580Y] to Cytokeratin 19
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: HeLa. ICC/IF KO: MCF7 cells (MCF7-KRT19 KO used as a negative cell line). Flow Cyt (intra): HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
  • Dissociation constant (KD)

    KD = 3.70 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1580Y
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class I
    • Cytokeratins

Associated products

  • Alternative Versions

    • Alexa Fluor® 647 Anti-Cytokeratin 19 antibody [EP1580Y] (ab192980)
    • HRP Anti-Cytokeratin 19 antibody [EP1580Y] (ab193600)
    • Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)
    • Alexa Fluor® 594 Anti-Cytokeratin 19 antibody [EP1580Y] (ab203443)
    • Alexa Fluor® 555 Anti-Cytokeratin 19 antibody [EP1580Y] (ab203444)
    • Alexa Fluor® 568 Anti-Cytokeratin 19 antibody [EP1580Y] (ab203445)
    • APC Anti-Cytokeratin 19 antibody [EP1580Y] (ab224980)
    • PE Anti-Cytokeratin 19 antibody [EP1580Y] (ab224981)
    • Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)
  • Isotype control

    • Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)
  • Related Products

    • Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab192643 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/500.
ICC/IF
1/100.

Signal can be observed in cells fixed with either methanol or paraformaldehyde.

Notes
Flow Cyt (Intra)
1/500.
ICC/IF
1/100.

Signal can be observed in cells fixed with either methanol or paraformaldehyde.

Target

  • Function

    Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
  • Tissue specificity

    Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin.
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Developmental stage

    Present in hair follicles at all stages of development.
  • Domain

    This keratin differs from all other IF proteins in lacking the C-terminal tail domain.
  • Target information above from: UniProt accession P08727 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3880 Human
    • Entrez Gene: 16669 Mouse
    • Omim: 148020 Human
    • SwissProt: P08727 Human
    • SwissProt: P19001 Mouse
    • Unigene: 654568 Human
    • Unigene: 439699 Mouse
    • Alternative names

      • 40 kDa keratin intermediate filament antibody
      • CK 19 antibody
      • CK-19 antibody
      • CK19 antibody
      • Cytokeratin 19 antibody
      • Cytokeratin-19 antibody
      • K19 antibody
      • K1C19_HUMAN antibody
      • K1CS antibody
      • Keratin 19 antibody
      • Keratin type I 40 kD antibody
      • Keratin type I 40kD antibody
      • Keratin type I cytoskeletal 19 antibody
      • Keratin, type I cytoskeletal 19 antibody
      • Keratin, type I, 40 kd antibody
      • Keratin-19 antibody
      • KRT19 antibody
      • MGC15366 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EP1580Y] (ab192643)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EP1580Y] (ab192643)

      Immunofluorescence staining of Cytokeratin 19 in wild-type MCF7 cells (top panel) and KRT19 knockout MCF7 cells (bottom panel) using ab192643. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab192643 at 1/500 dilution (shown in green) and ab195884 (Rat monoclonal to Tubulin - Alexa Fluor® 647) at 1/100 dilution (shown in red) overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.
      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EP1580Y] (ab192643)
      Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EP1580Y] (ab192643)

      Flow Cytometry analysis of Cytokeratin-19 in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained with ab192643 (red line).

      The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab192643, 1/500 dilution) for 30 min at 22°C.

      Isotype control antibody (black line) was rabbit monoclonal IgG [EPR25A] Alexa Fluor® 488 (ab199091) used at the same concentration and conditions as the primary antibody. Unlabeled sample (blue line) was also used as a control.

      Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EP1580Y] (ab192643)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EP1580Y] (ab192643)

      Immunofluorescence staining of Cytokeratin 19 in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells using ab192643. The cells were fixed with 4% formaldehyde (10 min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Triton X-100 for 1hr. The cells were then incubated with ab192643 at a working dilution of 1 in 100 (shown in green) and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin) at 1 µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1hr with an AlexaFluor® 594 Goat anti-mouse IgG (H&L - preadsorbed) secondary (ab150120) at 2 μg/ml (shown in pseudo-color red).

      Nuclear DNA was labeled in blue with DAPI.

      This product gave a positive signal in 100% methanol (5 min) fixed HeLa cells under the same testing conditions.

      Image was taken with a Confocal microscope (Leica-microsystems, TCS SP8)

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EP1580Y] (ab192643)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EP1580Y] (ab192643)Image from Wang et al, J Cell Mol Med 2014 Sep;18(9):1720-6. Fig 5, doi:10.1111/jcmm.12355.

      This data was produced using unconjugated parent ab52625.

      Immunocytochemistry analysis of mouse hepatic stem cells cells labelling CK-19 with ab52625 at 1/50 dilution. 

    • Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EP1580Y] (ab192643)
      Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EP1580Y] (ab192643)

    Protocols

    • Flow cytometry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab192643? Please let us know so that we can cite the reference in this datasheet.

    ab192643 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Flow Cytometry abreview for Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EP1580Y]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Mouse Cell (Thymic epithelial cells)
    Permeabilization
    Yes - Cytoperm buffer
    Gating Strategy
    CD45- Epcam+
    Specification
    Thymic epithelial cells
    Preparation
    Cell harvesting/tissue preparation method: Digest of thymi using liberase follwed by CD45 depletion
    Sample buffer: PBS containing 2% FCS
    Fixation
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Apr 11 2022

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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