RecombinantRabMAb

Recombinant Alexa Fluor® 488 Anti-EB3 antibody [EPR11421(B)] (ab203264)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-EB3 antibody [EPR11421(B)] (ab203264)
  • Alexa Fluor® 488 Anti-EB3 antibody [EPR11421(B)] (ab203264)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [EPR11421(B)] to EB3
  • Suitable for: ICC/IF
  • Reacts with: Mouse
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Overview

  • Product name

    Alexa Fluor® 488 Anti-EB3 antibody [EPR11421(B)]
    See all EB3 primary antibodies

  • Description

    Alexa Fluor® 488 Rabbit monoclonal [EPR11421(B)] to EB3

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: Neuro2a cells

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab203264 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Mouse
All applications
Rat
Human
Application Abreviews Notes
ICC/IF
1/200.

This product gave a positive signal in Neuro2a cells fixed with 100% methanol (5 min).
Notes
ICC/IF
1/200.

This product gave a positive signal in Neuro2a cells fixed with 100% methanol (5 min).

Target

  • Function

    May be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration.

  • Tissue specificity

    Predominantly expressed in brain and muscle.

  • Sequence similarities

    Belongs to the MAPRE family.
    Contains 1 CH (calponin-homology) domain.
    Contains 1 EB1 C-terminal domain.

  • Domain

    Composed of two functionally independent domains. The N-terminal domain forms an hydrophobic cleft involved in microtubule binding and the C-terminal is involved in the formation of mutually exclusive complexes with APC and DCTN1.

  • Cellular localization

    Cytoplasm > cytoskeleton. Associated with the microtubule network.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • APC binding protein antibody
    • EB 3 antibody
    • EB1 protein family member 3 antibody
    • EB3 antibody
    • EBF 3 antibody
    • EBF-3 antibody
    • EBF3 antibody
    • EBF3 S antibody
    • End binding protein 3 antibody
    • End-binding protein 3 antibody
    • MAPRE 3 antibody
    • MAPRE3 antibody
    • MARE3_HUMAN antibody
    • Member 3 antibody
    • Microtubule Associated Protein antibody
    • Microtubule associated protein RP/EB family member 3 antibody
    • Microtubule-associated protein RP/EB family member 3 antibody
    • OTTHUMP00000200910 antibody
    • RP 3 antibody
    • RP/EB Family antibody
    • RP3 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-EB3 antibody [EPR11421(B)] (ab203264)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-EB3 antibody [EPR11421(B)] (ab203264)

    ab203264 staining EB3 in Neuro2a cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab203264 at 1/200 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Alexa Fluor® 488 Anti-EB3 antibody [EPR11421(B)] (ab203264)
    Alexa Fluor® 488 Anti-EB3 antibody [EPR11421(B)] (ab203264)

References (0)

Publishing research using ab203264? Please let us know so that we can cite the reference in this datasheet.

ab203264 has not yet been referenced specifically in any publications.

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