RecombinantRabMAb

Recombinant Alexa Fluor® 488 Anti-EB3 antibody [EPR11421(B)] (ab203264)

Submit a review Submit a question
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-EB3 antibody [EPR11421(B)] (ab203264)
  • Alexa Fluor® 488 Anti-EB3 antibody [EPR11421(B)] (ab203264)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [EPR11421(B)] to EB3
  • Suitable for: ICC/IF
  • Reacts with: Mouse
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Overview

  • Product name

    Alexa Fluor® 488 Anti-EB3 antibody [EPR11421(B)]
    See all EB3 primary antibodies

  • Description

    Alexa Fluor® 488 Rabbit monoclonal [EPR11421(B)] to EB3

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

  • Tested applications

    Suitable for: ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Human

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human EB3 aa 250 to the C-terminus.
    Database link: Q9UPY8

  • Positive control

    • ICC/IF: Neuro2a cells

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab203264 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.

This product gave a positive signal in Neuro2a cells fixed with 100% methanol (5 min).

Target

  • Function

    May be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration.

  • Tissue specificity

    Predominantly expressed in brain and muscle.

  • Sequence similarities

    Belongs to the MAPRE family.
    Contains 1 CH (calponin-homology) domain.
    Contains 1 EB1 C-terminal domain.

  • Domain

    Composed of two functionally independent domains. The N-terminal domain forms an hydrophobic cleft involved in microtubule binding and the C-terminal is involved in the formation of mutually exclusive complexes with APC and DCTN1.

  • Cellular localization

    Cytoplasm > cytoskeleton. Associated with the microtubule network.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • APC binding protein antibody
    • EB 3 antibody
    • EB1 protein family member 3 antibody
    • EB3 antibody
    • EBF 3 antibody
    • EBF-3 antibody
    • EBF3 antibody
    • EBF3 S antibody
    • End binding protein 3 antibody
    • End-binding protein 3 antibody
    • MAPRE 3 antibody
    • MAPRE3 antibody
    • MARE3_HUMAN antibody
    • Member 3 antibody
    • Microtubule Associated Protein antibody
    • Microtubule associated protein RP/EB family member 3 antibody
    • Microtubule-associated protein RP/EB family member 3 antibody
    • OTTHUMP00000200910 antibody
    • RP 3 antibody
    • RP/EB Family antibody
    • RP3 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-EB3 antibody [EPR11421(B)] (ab203264)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-EB3 antibody [EPR11421(B)] (ab203264)

    ab203264 staining EB3 in Neuro2a cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab203264 at 1/200 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Alexa Fluor® 488 Anti-EB3 antibody [EPR11421(B)] (ab203264)
    Alexa Fluor® 488 Anti-EB3 antibody [EPR11421(B)] (ab203264)

References (0)

Publishing research using ab203264? Please let us know so that we can cite the reference in this datasheet.

ab203264 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab203264.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com