Recombinant Alexa Fluor® 488 Anti-EpCAM antibody [EPR20533-63] (ab237384)
![Flow Cytometry - Alexa Fluor® 488 Anti-EpCAM antibody [EPR20533-63] (ab237384)](https://www.abcam.com/ps/products/237/ab237384/Images/ab237384-399003-ab237384-Rabbit-monoclonal-EPR2053363-EpCAM-Alexa-Fluor-488-Mouse-M158-cells-flow-cytometry.jpg "Flow Cytometry - Alexa Fluor® 488 Anti-EpCAM antibody [EPR20533-63] (ab237384)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 488 Rabbit monoclonal [EPR20533-63] to EpCAM
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Mouse
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Overview
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Product name
Alexa Fluor® 488 Anti-EpCAM antibody [EPR20533-63]
See all EpCAM primary antibodies -
Description
Alexa Fluor® 488 Rabbit monoclonal [EPR20533-63] to EpCAM -
Host species
Rabbit -
Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
Tested applications
Suitable for: Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment within Mouse EpCAM aa 1-300. The exact sequence is proprietary.
Database link: Q99JW5 -
Positive control
- Flow Cyt: 4T1 cells. ICC/IF: 4T1 and M158 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol, 1% BSA, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
EPR20533-63 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab237384 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/50 - 1/500. | |
| ICC/IF | 1/200. This product gave a positive signal in 4T1 cells fixed with 100% methanol (5 min) |
Target
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Function
May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E. -
Tissue specificity
Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC). Levels rapidly diminish as soon as ESC's differentiate (at protein levels). Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma. -
Involvement in disease
Defects in EPCAM are the cause of diarrhea type 5 (DIAR5) [MIM:613217]. It is an intractable diarrhea of infancy characterized by villous atrophy and absence of inflammation, with intestinal epithelial cell dysplasia manifesting as focal epithelial tufts in the duodenum and jejunum.
Defects in EPCAM are a cause of hereditary non-polyposis colorectal cancer type 8 (HNPCC8) [MIM:613244]. HNPCC is a disease associated with marked increase in cancer susceptibility. It is characterized by a familial predisposition to early-onset colorectal carcinoma (CRC) and extra-colonic tumors of the gastrointestinal, urological and female reproductive tracts. HNPCC is reported to be the most common form of inherited colorectal cancer in the Western world. Clinically, HNPCC is often divided into two subgroups. Type I is characterized by hereditary predisposition to colorectal cancer, a young age of onset, and carcinoma observed in the proximal colon. Type II is characterized by increased risk for cancers in certain tissues such as the uterus, ovary, breast, stomach, small intestine, skin, and larynx in addition to the colon. Diagnosis of classical HNPCC is based on the Amsterdam criteria: 3 or more relatives affected by colorectal cancer, one a first degree relative of the other two; 2 or more generation affected; 1 or more colorectal cancers presenting before 50 years of age; exclusion of hereditary polyposis syndromes. The term 'suspected HNPCC' or 'incomplete HNPCC' can be used to describe families who do not or only partially fulfill the Amsterdam criteria, but in whom a genetic basis for colon cancer is strongly suspected. Note=HNPCC8 results from heterozygous deletion of 3-prime exons of EPCAM and intergenic regions directly upstream of MSH2, resulting in transcriptional read-through and epigenetic silencing of MSH2 in tissues expressing EPCAM. -
Sequence similarities
Belongs to the EPCAM family.
Contains 1 thyroglobulin type-1 domain. -
Post-translational
modificationsHyperglycosylated in carcinoma tissue as compared with autologous normal epithelia. Glycosylation at Asn-198 is crucial for protein stability. -
Cellular localization
Lateral cell membrane. Cell junction > tight junction. Co-localizes with CLDN7 at the lateral cell membrane and tight junction. - Information by UniProt
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Database links
- Entrez Gene: 17075 Mouse
- SwissProt: Q99JW5 Mouse
- Unigene: 4259 Mouse
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Alternative names
- 17 1A antibody
- 323/A3 antibody
- Adenocarcinoma associated antigen antibody
see all
Images
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Flow Cytometry - Alexa Fluor® 488 Anti-EpCAM antibody [EPR20533-63] (ab237384)
Flow cytometry overlay histogram showing M158 positive cells (left) and negative NIH/3T3 cells (right) stained with ab237384 (red line). The cells were incubated in 1x PBS containing 10 % normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab237384) (1x 106 in 100 µl at 1 µg/ml (1/500)) for 30 min on ice.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor 488® (ab199091) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.
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![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-EpCAM antibody [EPR20533-63] (ab237384)](https://www.abcam.com/ps/products/237/ab237384/Images/ab237384-339654-ICCAP00413542.jpg)
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-EpCAM antibody [EPR20533-63] (ab237384)ab237384 staining EpCAM in 4T1 cells. The cells were fixed with 100% methanol (5 min), and then incubated in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions.
The cells were then incubated overnight at +4°C with ab237384 at 1/200 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-EpCAM antibody [EPR20533-63] (ab237384)](https://www.abcam.com/ps/products/237/ab237384/Images/ab237384-339653-ICCAP00413541.jpg)
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-EpCAM antibody [EPR20533-63] (ab237384)ab237384 showing no staining of EpCAM in NIH3T3 cells. The cells were fixed with 100% methanol (5 min), and then incubated in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions.
The cells were then incubated overnight at +4°C with ab237384 at 1/200 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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![Flow Cytometry - Alexa Fluor® 488 Anti-EpCAM antibody [EPR20533-63] (ab237384)](https://www.abcam.com/ps/products/237/ab237384/Images/ab237384-339511-ab237384FLOW1.jpg)
Flow Cytometry - Alexa Fluor® 488 Anti-EpCAM antibody [EPR20533-63] (ab237384)Flow cytometric analysis of unfixed 4T1 (right) and NIH/3T3 (left) cells with ab2237384 (red line). The cells were incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab237384, 1/50 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor® 488 (ab199091) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 20mW solid-state laser (488nm) and 530/30 bandpass filter.
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![Alexa Fluor® 488 Anti-EpCAM antibody [EPR20533-63] (ab237384)](https://www.abcam.com/ps/products/237/ab237384/Images/ab237384-5-benefits-of-recombinant-antibodies.png)
Alexa Fluor® 488 Anti-EpCAM antibody [EPR20533-63] (ab237384)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab237384 has not yet been referenced specifically in any publications.
