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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Methylation
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RecombinantRabMAb

Recombinant Alexa Fluor® 488 Anti-ERG antibody [EPR3864] (ab196374)

  • Datasheet
  • Certificate of Compliance
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-ERG antibody [EPR3864] (ab196374)
  • Alexa Fluor® 488 Anti-ERG antibody [EPR3864] (ab196374)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [EPR3864] to ERG
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

You may also be interested in

Primary
Product image
Alexa Fluor® 647 Anti-ERG antibody [EPR3864] (ab196149)

View more associated products

Overview

  • Product name

    Alexa Fluor® 488 Anti-ERG antibody [EPR3864]
    See all ERG primary antibodies
  • Description

    Alexa Fluor® 488 Rabbit monoclonal [EPR3864] to ERG
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human ERG aa 450 to the C-terminus.
    Database link: P11308

  • Positive control

    • ICC/IF: THP-1 cells
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Dissociation constant (KD)

    KD = 8.90 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 30% Glycerol, 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR3864
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Methylation
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Proto-oncogenes
    • Stem Cells
    • Hematopoietic Progenitors
    • Intracellular Molecules
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Methylation
    • Lysine methylation

Associated products

  • Alternative Versions

    • HRP Anti-ERG antibody [EPR3864] (ab195514)
    • Alexa Fluor® 647 Anti-ERG antibody [EPR3864] (ab196149)
    • Anti-ERG antibody [EPR3864] - BSA and Azide free (ab214796)
    • Anti-ERG antibody [EPR3864] (ab92513)
  • Isotype control

    • Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)
  • Positive Controls

    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant Human ERG protein (ab185597)

Applications

Our Abpromise guarantee covers the use of ab196374 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.

This product gave a positive signal in THP-1 cells fixed with 4% formaldehyde (10 min)

Target

  • Function

    Transcriptional regulator. May participate in transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of local chromatin structure.
  • Involvement in disease

    Defects in ERG are a cause of Ewing sarcoma (ES) [MIM:612219]. A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. Note=A chromosomal aberration involving ERG is found in patients with Erwing sarcoma. Translocation t(21;22)(q22;q12) with EWSR1.
    Note=Chromosomal aberrations involving ERG have been found in acute myeloid leukemia (AML). Translocation t(16;21)(p11;q22) with FUS. Translocation t(X;21)(q25-26;q22) with ELF4.
  • Sequence similarities

    Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
    Contains 1 PNT (pointed) domain.
  • Cellular localization

    Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Target information above from: UniProt accession P11308 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2078 Human
    • Entrez Gene: 13876 Mouse
    • Entrez Gene: 170909 Rat
    • Omim: 165080 Human
    • SwissProt: P11308 Human
    • SwissProt: P81270 Mouse
    • Unigene: 473819 Human
    • Unigene: 164531 Mouse
    • Unigene: 47987 Rat
    see all
  • Alternative names

    • Avian erythroblastosis virus E-26 (v-ets) oncogene related antibody
    • D030036I24Rik antibody
    • Erg 3 antibody
    • Erg antibody
    • ERG/EWS fusion gene, included antibody
    • ERG/FUS fusion gene, included antibody
    • ERG/TMPSSR2 fusion gene, included antibody
    • ERG_HUMAN antibody
    • ERG1, included antibody
    • ERG2, included antibody
    • ets related antibody
    • ETS-related gene antibody
    • KCNH2 antibody
    • Oncogene ERG antibody
    • p55 antibody
    • TMPRSS2/ERG fusion antibody
    • transcriptional regulator ERG (transforming protein ERG) antibody
    • Transcriptional regulator ERG antibody
    • Transforming protein ERG antibody
    • v ets avian erythroblastosis virus E26 oncogene antibody
    • v ets avian erythroblastosis virus E26 oncogene related antibody
    • v ets erythroblastosis virus E26 oncogene homolog antibody
    • v ets erythroblastosis virus E26 oncogene like antibody
    • v ets erythroblastosis virus E26 oncogene like isoform 2 antibody
    • v-ets erythroblastosis virus E26 oncogene antibody
    • v-ets erythroblastosis virus E26 oncogene homolog (avian) antibody
    • V-ets erythroblastosis virus E26 oncogene like (Avian), isoform CRA_e antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-ERG antibody [EPR3864] (ab196374)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-ERG antibody [EPR3864] (ab196374)

    ab196374 staining ERG in THP-1 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196374 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Alexa Fluor® 488 Anti-ERG antibody [EPR3864] (ab196374)
    Alexa Fluor® 488 Anti-ERG antibody [EPR3864] (ab196374)

Protocols

  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab196374? Please let us know so that we can cite the reference in this datasheet.

    ab196374 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunohistochemistry (Frozen sections) abreview for Anti-ERG antibody [EPR3864] (Alexa Fluor® 488)

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Lymph node)
    Permeabilization
    Yes - 0.05% Tween, 0.1% Triton
    Specification
    Lymph node
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 16 2018

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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