Alexa Fluor® 488 Anti-F4/80 antibody [F4/80] (ab204266)
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-F4/80 antibody [F4/80] (ab204266)](https://www.abcam.com/ps/products/204/ab204266/Images/ab204266-319608-anti-f4-80-antibody-f4-80-alexa-fluor-488-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-F4/80 antibody [F4/80] (ab204266)")
Key features and details
- Alexa Fluor® 488 Rat monoclonal [F4/80] to F4/80
- Suitable for: ICC/IF
- Reacts with: Mouse
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
- Isotype: IgG2b
Overview
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Product name
Alexa Fluor® 488 Anti-F4/80 antibody [F4/80]
See all F4/80 primary antibodies -
Description
Alexa Fluor® 488 Rat monoclonal [F4/80] to F4/80 -
Host species
Rat -
Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Mouse F4/80. (Thioglycollate stimulated peritoneal macrophages from C57/BL mice).
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Positive control
- ICC/IF: Raw264.7
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General notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol, 1% BSA, PBS -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
F4/80 -
Myeloma
NS1 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab204266 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100. This product gave a positive signal in Raw264.7 cells fixed with 100% methanol (5 min). |
Target
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Function
Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development. -
Tissue specificity
Expression is restricted to eosinophils. -
Sequence similarities
Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
Contains 6 EGF-like domains.
Contains 1 GPS domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 13733 Mouse
- SwissProt: Q61549 Mouse
- Unigene: 2254 Mouse
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Alternative names
- ADGRE1 antibody
- Adhesion G protein coupled receptor E1 antibody
- Adhesion G protein-coupled receptor E1 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-F4/80 antibody [F4/80] (ab204266)
ab204266 staining F4/80 in Raw264.7 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab204266 at a 1/40 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Protocols
Datasheets and documents
References (3)
ab204266 has been referenced in 3 publications.
- Swiderski K et al. Deletion of suppressor of cytokine signaling 3 (SOCS3) in muscle stem cells does not alter muscle regeneration in mice after injury. PLoS One 14:e0212880 (2019). PubMed: 30811469
- Liu W et al. Multiple Immunosuppressive Effects of CpG-c41 on Intracellular TLR-Mediated Inflammation. Mediators Inflamm 2017:6541729 (2017). ICC/IF ; Mouse . PubMed: 28539706
- Jiang C et al. Targeted Imaging of Tumor-Associated Macrophages by Cyanine 7-Labeled Mannose in Xenograft Tumors. Mol Imaging 16:1536012116689499 (2017). PubMed: 28654380
