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RecombinantRabMAb

Recombinant Alexa Fluor® 488 Anti-Fibronectin antibody [F1] (ab198933)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (1) Submit a question References (4)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Fibronectin antibody [F1] (ab198933)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Fibronectin antibody [F1] (ab198933)
  • Flow Cytometry - Alexa Fluor® 488 Anti-Fibronectin antibody [F1] (ab198933)
  • Alexa Fluor® 488 Anti-Fibronectin antibody [F1] (ab198933)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [F1] to Fibronectin
  • Suitable for: IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

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Alexa Fluor® 647 Anti-Fibronectin antibody [F1] (ab198934)
Isotype
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Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

View more associated products

Overview

  • Product name

    Alexa Fluor® 488 Anti-Fibronectin antibody [F1]
    See all Fibronectin primary antibodies
  • Description

    Alexa Fluor® 488 Rabbit monoclonal [F1] to Fibronectin
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein within Fibronectin aa 1-2400. The exact sequence is proprietary.
    Database link: P02751

  • Positive control

    • ICC/IF: A431 cells. Flow Cyt: A431 cells. IHC-P: Human normal tonsil tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 30% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    F1
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Adhesion / ECM
    • Extracellular Matrix
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Proteins
    • Fibronectin
    • Stem Cells
    • Lineage Markers
    • Ectoderm
    • Stem Cells
    • Neural Stem Cells
    • Extracellular
    • Stem Cells
    • Mesenchymal Stem Cells
    • Osteogenesis
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Extracellular matrix
    • Other
    • Developmental Biology
    • Lineage specification
    • Ectoderm

Associated products

  • Alternative Versions

    • Alexa Fluor® 647 Anti-Fibronectin antibody [F1] (ab198934)
    • Anti-Fibronectin antibody [F1] - Low endotoxin, Azide free (ab219366)
    • Alexa Fluor® 555 Anti-Fibronectin antibody [F1] (ab275110)
    • Anti-Fibronectin antibody [F1] (ab32419)
  • Isotype control

    • Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)
  • Recombinant Protein

    • Recombinant Human Fibronectin type III EDB protein (ab209886)
  • Related Products

    • Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab195884)

Applications

Our Abpromise guarantee covers the use of ab198933 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100.

This product gave a positive signal in A431 cells fixed with 4% formaldehyde (10 minutes).

Flow Cyt 1/50.

Target

  • Function

    Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
    Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
  • Tissue specificity

    Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.
  • Involvement in disease

    Glomerulopathy with fibronectin deposits 2
  • Sequence similarities

    Contains 12 fibronectin type-I domains.
    Contains 2 fibronectin type-II domains.
    Contains 16 fibronectin type-III domains.
  • Developmental stage

    Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
  • Post-translational
    modifications

    Sulfated.
    It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
    Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
    Phosphorylated by FAM20C in the extracellular medium.
    Proteolytic processing produces the C-terminal NC1 peptide, anastellin.
  • Cellular localization

    Secreted, extracellular space, extracellular matrix.
  • Target information above from: UniProt accession P02751 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2335 Human
    • Omim: 135600 Human
    • SwissProt: P02751 Human
    • Unigene: 203717 Human
    • Alternative names

      • CIG antibody
      • Cold insoluble globulin antibody
      • Cold-insoluble globulin antibody
      • DKFZp686F10164 antibody
      • DKFZp686H0342 antibody
      • DKFZp686I1370 antibody
      • DKFZp686O13149 antibody
      • ED B antibody
      • Fibronectin 1 antibody
      • FINC antibody
      • FINC_HUMAN antibody
      • FN antibody
      • FN1 antibody
      • FNZ antibody
      • GFND antibody
      • GFND2 antibody
      • LETS antibody
      • Migration stimulating factor antibody
      • MSF antibody
      • Ugl-Y3 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Fibronectin antibody [F1] (ab198933)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Fibronectin antibody [F1] (ab198933)

      Immunofluorescence staining of PD1 staining in a section of formalin-fixed paraffin-embedded human normal tonsil*.

      The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) in a Biocare Medical NxGen pressure cooker using retrieval settings of 110°C for 20 minutes. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab198933 at 1/100 dilution (shown in green) and counterstained using ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Fibronectin antibody [F1] (ab198933)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Fibronectin antibody [F1] (ab198933)

      ab198933 staining Fibronectin in A431 (Human epidermoid carcinoma cell line) cells. The cells were fixed with 4% formaldehyde (10 minutes), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1 hour. The cells were then incubated overnight at +4°C with ab198933 at a 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Flow Cytometry - Alexa Fluor® 488 Anti-Fibronectin antibody [F1] (ab198933)
      Flow Cytometry - Alexa Fluor® 488 Anti-Fibronectin antibody [F1] (ab198933)

      Overlay histogram showing A431 (Human epidermoid carcinoma cell line) cells stained with ab198933 (red line). The cells were fixed with 4% formaldehyde (10 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab198933, 1/50 dilution) for 30 minutes at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG [EPR25A] Alexa Fluor® 488 (ab199091) used at the same concentration and conditions as the primary antibody. Unlabeled sample (blue line) was also used as a control.

      Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

      This antibody gave a positive signal in A431 cells fixed with 80% methanol (5 minutes)/permeabilized with 0.1% PBS-Tween for 20 minutes used under the same conditions.

    • Alexa Fluor® 488 Anti-Fibronectin antibody [F1] (ab198933)
      Alexa Fluor® 488 Anti-Fibronectin antibody [F1] (ab198933)

    Protocols

    • Flow cytometry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (4)

    Publishing research using ab198933? Please let us know so that we can cite the reference in this datasheet.

    ab198933 has been referenced in 4 publications.

    • Rashid R  et al. Highly multiplexed immunofluorescence images and single-cell data of immune markers in tonsil and lung cancer. Sci Data 6:323 (2019). beta actin ; Human . PubMed: 31848351
    • Middleton RC  et al. Newt cells secrete extracellular vesicles with therapeutic bioactivity in mammalian cardiomyocytes. J Extracell Vesicles 7:1456888 (2018). PubMed: 29696078
    • Lin JR  et al. Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes. Elife 7:N/A (2018). PubMed: 29993362
    • Belair DG  et al. Engineering human cell spheroids to model embryonic tissue fusion in vitro. PLoS One 12:e0184155 (2017). PubMed: 28898253

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Flow Cytometry abreview for Anti-Fibronectin antibody [F1] (Alexa Fluor® 488)

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Mouse Cell (bone marrow derived macrophages)
    Permeabilization
    Yes - 0.1% saponin
    Gating Strategy
    live cells --> F4/80 positive
    Specification
    bone marrow derived macrophages
    Preparation
    Cell harvesting/tissue preparation method: Murine bone marrow cells were seeded and were treated with M-CSF through 8 days (M0 macrophages). M1 macrophages were stimulated with IFN-gamma and M2 macrophages were stimulated with IL-4+IL-13.
    Sample buffer: PBS
    Fixation
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted May 26 2020

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