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    alexa-fluor-488-fkbp51-antibody-epr6617-ab198978.pdf

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Immunology Immune Interventions Transplantation
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RecombinantRabMAb

Recombinant Alexa Fluor® 488 Anti-FKBP51 antibody [EPR6617] (ab198978)

  • Datasheet
  • Certificate of Compliance
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Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-FKBP51 antibody [EPR6617] (ab198978)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-FKBP51 antibody [EPR6617] (ab198978)
  • Alexa Fluor® 488 Anti-FKBP51 antibody [EPR6617] (ab198978)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [EPR6617] to FKBP51
  • Suitable for: ICC/IF, Flow Cyt (Intra)
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 Carrier Free Unconjugated

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Alexa Fluor® 647 Anti-FKBP51 antibody [EPR6617] (ab198979)
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Alexa Fluor® 488 Conjugation Kit (Fast) - Lightning-Link® (ab236553)
Primary
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Anti-FKBP51 antibody [EPR6617] - BSA and Azide free (ab242392)

View more associated products

Overview

  • Product name

    Alexa Fluor® 488 Anti-FKBP51 antibody [EPR6617]
    See all FKBP51 primary antibodies
  • Description

    Alexa Fluor® 488 Rabbit monoclonal [EPR6617] to FKBP51
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested applications

    Suitable for: ICC/IF, Flow Cyt (Intra)more details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: Hela cells. Flow Cyt (intra): HeLa cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Dissociation constant (KD)

    KD = 7.10 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 1% BSA, 30% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR6617
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Immune Interventions
    • Transplantation
    • Signal Transduction
    • Protein Trafficking
    • Nuclear Import / Export

Associated products

  • Alternative Versions

    • Anti-FKBP51 antibody [EPR6617] (ab126715)
    • Alexa Fluor® 647 Anti-FKBP51 antibody [EPR6617] (ab198979)
    • Anti-FKBP51 antibody [EPR6617] - BSA and Azide free (ab242392)
  • Isotype control

    • Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)
  • Positive Controls

    • Jurkat cytoplasmic extract lysate (ab14842)
  • Recombinant Protein

    • Recombinant Human FKBP51 protein (His tag) (ab206440)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab198978 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/100.
Flow Cyt (Intra)
1/500.
Notes
ICC/IF
1/100.
Flow Cyt (Intra)
1/500.

Target

  • Function

    Interacts with functionally mature heterooligomeric progesterone receptor complexes along with HSP90 and TEBP.
  • Tissue specificity

    Widely expressed, enriched in testis compared to other tissues.
  • Sequence similarities

    Contains 2 PPIase FKBP-type domains.
    Contains 3 TPR repeats.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Target information above from: UniProt accession Q13451 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2289 Human
    • Omim: 602623 Human
    • SwissProt: Q13451 Human
    • Unigene: 407190 Human
    • Alternative names

      • 51 kDa FK506 binding protein 5 antibody
      • 51 kDa FK506 binding protein antibody
      • 51 kDa FK506-binding protein antibody
      • 51 kDa FKBP antibody
      • 54 kDa progesterone receptor associated immunophilin antibody
      • 54 kDa progesterone receptor-associated immunophilin antibody
      • AIG 6 antibody
      • AIG6 antibody
      • Androgen regulated protein 6 antibody
      • Androgen-regulated protein 6 antibody
      • FF1 antigen antibody
      • FK506 binding protein 5 antibody
      • FK506-binding protein 5 antibody
      • FKBP 5 antibody
      • FKBP 51 antibody
      • FKBP 54 antibody
      • FKBP-5 antibody
      • FKBP-51 antibody
      • FKBP5 antibody
      • FKBP5_HUMAN antibody
      • FKBP54 antibody
      • HSP90 binding immunophilin antibody
      • HSP90-binding immunophilin antibody
      • MGC111006 antibody
      • OTTHUMP00000016268 antibody
      • P54 antibody
      • Peptidyl prolyl cis trans isomerase antibody
      • Peptidyl-prolyl cis-trans isomerase FKBP5 antibody
      • Peptidylprolyl cis trans isomerase antibody
      • PPIase antibody
      • PPIase FKBP5 antibody
      • Ptg 10 antibody
      • Ptg10 antibody
      • Rotamase antibody
      • T cell FK506 binding protein antibody
      see all

    Images

    • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-FKBP51 antibody [EPR6617] (ab198978)
      Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-FKBP51 antibody [EPR6617] (ab198978)

      Overlay histogram showing HeLa cells stained with ab198978 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab198978, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 (ab199091) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

      Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

      This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-FKBP51 antibody [EPR6617] (ab198978)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-FKBP51 antibody [EPR6617] (ab198978)

      ab198978 staining FKBP51 in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198978 at 1/100 dilution (shown in green) and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin) at 1µg/ml. This was followed by an incubation at room temperature for 1h with ab150120, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed, at 1µg/ml (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Alexa Fluor® 488 Anti-FKBP51 antibody [EPR6617] (ab198978)
      Alexa Fluor® 488 Anti-FKBP51 antibody [EPR6617] (ab198978)

    Protocols

    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab198978? Please let us know so that we can cite the reference in this datasheet.

    ab198978 has not yet been referenced specifically in any publications.

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