Alexa Fluor® 488 Anti-FOXP3 antibody [3G3] (ab187598)
![Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-FOXP3 antibody [3G3] (ab187598)](https://www.abcam.com/ps/products/187/ab187598/Images/ab187598-455332-alexa-fluor-488-anti-foxp3-antibody-3g3-flow-cytometry-intracellular-peripheral-blood-human.jpg "Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-FOXP3 antibody [3G3] (ab187598)")
Key features and details
- Alexa Fluor® 488 Mouse monoclonal [3G3] to FOXP3
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
- Isotype: IgG1
Overview
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Product name
Alexa Fluor® 488 Anti-FOXP3 antibody [3G3]
See all FOXP3 primary antibodies -
Description
Alexa Fluor® 488 Mouse monoclonal [3G3] to FOXP3 -
Host species
Mouse -
Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt (Intra) HumanICC/IF Human
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Immunogen
Recombinant full length protein (His-tag) corresponding to Mouse FOXP3 aa 1 to the C-terminus.
Database link: Q99JB6 -
Positive control
- Flow Cyt (Intra): Human peripheral whole blood.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Size exclusion -
Purification notes
Purified antibody is conjugated with Alexa Fluor® 488 under optimum conditions. The conjugate is purified by size-exclusion chromatography. -
Clonality
Monoclonal -
Clone number
3G3 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab187598 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Flow Cyt (Intra) |
Human
|
| ICC/IF |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
Use a concentration of 1 - 5 µg/ml.
|
| Notes |
|---|
|
Flow Cyt (Intra)
Use a concentration of 1 - 5 µg/ml. |
Target
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Function
Probable transcription factor. Plays a critical role in the control of immune response. -
Involvement in disease
Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [MIM:304790]; also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. It is usually lethal in infancy. -
Sequence similarities
Contains 1 C2H2-type zinc finger.
Contains 1 fork-head DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 50943 Human
- Omim: 300292 Human
- SwissProt: Q9BZS1 Human
- Unigene: 247700 Human
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Alternative names
- AIID antibody
- DIETER antibody
- Forkhead box P3 antibody
see all
Images
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Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-FOXP3 antibody [3G3] (ab187598)
Flow Cytometry (Intracellular) analysis of human peripheral whole blood labeling FOXP3 with ab187598 at 3 μg/mL.
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![Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-FOXP3 antibody [3G3] (ab187598)](https://www.abcam.com/ps/products/187/ab187598/Images/ab187598-455331-alexa-fluor-488-anti-foxp3-antibody-3g3-flow-cytometry-intracellular-cd25-cd4-peripheral-blood-human.jpg)
Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-FOXP3 antibody [3G3] (ab187598)Flow cytometry multicolor surface staining of human CD4 positive lymphocytes using anti-human CD25 (MEM-181) APC antibody (10 μL reagent / 100 μL of peripheral whole blood) and intracellular staining of human CD4 positive lymphocytes using ab187598 at 3 μg/mL.
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![Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-FOXP3 antibody [3G3] (ab187598)](https://www.abcam.com/ps/products/187/ab187598/Images/ab187598-455330-alexa-fluor-488-anti-foxp3-antibody-3g3-flow-cytometry-intracellular-cd4-peripheral-blood-human.jpg)
Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-FOXP3 antibody [3G3] (ab187598)Flow cytometry multicolor surface staining of human lymphocytes using anti-human CD4 (MEM-241) Pacific Blue™ antibody (4 μL reagent / 100 μL of peripheral whole blood) and intracellular staining of human lymphocytes using ab187598 at 3 μg/mL.
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![Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-FOXP3 antibody [3G3] (ab187598)](https://www.abcam.com/ps/products/187/ab187598/Images/ab187598-455329-alexa-fluor-488-anti-foxp3-antibody-3g3-flow-cytometry-intracellular-histogram-cd4-peripheral-blood-human.jpg)
Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-FOXP3 antibody [3G3] (ab187598)Flow Cytometry (Intracellular) analysis of CD25 positive, CD4 positive lymphocytes in human peripheral whole blood labeling FOXP3 with ab187598 at 3 μg/mL (red).
Negative control: FoxP3 negative, CD25 negative, CD4 positive lymphocytes (black-dashed).
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab187598 has not yet been referenced specifically in any publications.