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RecombinantRabMAb

Recombinant Alexa Fluor® 488 Anti-gamma Tubulin antibody [EPR16793] - Centrosome Marker (ab205475)

Reviews (2) Submit a question References (1)
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-gamma Tubulin antibody [EPR16793] - Centrosome Marker (ab205475)
  • Alexa Fluor® 488 Anti-gamma Tubulin antibody [EPR16793] - Centrosome Marker (ab205475)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [EPR16793] to gamma Tubulin - Centrosome Marker
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Conjugates logo Related conjugates and formulations

Carrier Free Unconjugated

Overview

  • Product name

    Alexa Fluor® 488 Anti-gamma Tubulin antibody [EPR16793] - Centrosome Marker
    See all gamma Tubulin primary antibodies

  • Description

    Alexa Fluor® 488 Rabbit monoclonal [EPR16793] to gamma Tubulin - Centrosome Marker

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

  • Tested applications

    Suitable for: ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Hamster, Cow, Dog, Fish, Monkey, Xenopus tropicalis

  • Immunogen

    Recombinant fragment corresponding to Human gamma Tubulin aa 200 to the C-terminus.
    Database link: P23258

  • Positive control

    • ICC/IF: HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab205475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF (1)
1/500.
Notes
ICC/IF
1/500.

Target

Images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-gamma Tubulin antibody [EPR16793] - Centrosome Marker (ab205475)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-gamma Tubulin antibody [EPR16793] - Centrosome Marker (ab205475)

    ab205475 staining gamma Tubulin in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab205475 at a 1/500 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Alexa Fluor® 488 Anti-gamma Tubulin antibody [EPR16793] - Centrosome Marker (ab205475)
    Alexa Fluor® 488 Anti-gamma Tubulin antibody [EPR16793] - Centrosome Marker (ab205475)

References (1)

Publishing research using ab205475? Please let us know so that we can cite the reference in this datasheet.

ab205475 has been referenced in 1 publication.

  • Roberts MS  et al. LIN9 and NEK2 Are Core Regulators of Mitotic Fidelity That Can Be Therapeutically Targeted to Overcome Taxane Resistance. Cancer Res 80:1693-1706 (2020). PubMed: 32054769

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Flow Cytometry abreview for Alexa Fluor® 488 Anti-gamma Tubulin antibody [EPR16793]

 Excellent
Abreviews
abreview image
Application
Flow Cytometry
Sample
Mouse Cell (white blood cells)
Permeabilization
Yes - Intracellular Fixation & Permeabilization Buffer Set(eBioscience)
Gating Strategy
white blood cells
Specification
white blood cells
Preparation
Cell harvesting/tissue preparation method: RBC lysis buffer (eBioscience)
Sample buffer: PBS/2%FBS
Fixation
Intracellular Fixation & Permeabilization Buffer Set(eBioscience)
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 09 2022

Immunocytochemistry/ Immunofluorescence abreview for Alexa Fluor® 488 Anti-gamma Tubulin antibody [EPR16793]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (R28)
Specification
R28
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 14 2019

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