Recombinant Alexa Fluor® 488 Anti-Glutamine Synthetase antibody [EPR13022(B)] (ab302584)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 488 Rabbit monoclonal [EPR13022(B)] to Glutamine Synthetase
- Suitable for: IHC-P
- Reacts with: Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Related conjugates and formulations
Overview
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Product name
Alexa Fluor® 488 Anti-Glutamine Synthetase antibody [EPR13022(B)]
See all Glutamine Synthetase primary antibodies -
Description
Alexa Fluor® 488 Rabbit monoclonal [EPR13022(B)] to Glutamine Synthetase -
Host species
Rabbit -
Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
Tested applications
Suitable for: IHC-Pmore details
Unsuitable for: IHC-Fr -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human glioma and human breast tumor FFPE tissue sections.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13022(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- HRP Anti-Glutamine Synthetase antibody [EPR13022(B)] (ab199198)
- Anti-Glutamine Synthetase antibody [EPR13022(B)] - BSA and Azide free (ab240193)
- Alexa Fluor® 555 Anti-Glutamine Synthetase antibody [EPR13022(B)] (ab302572)
- Alexa Fluor® 568 Anti-Glutamine Synthetase antibody [EPR13022(B)] (ab302807)
- Alexa Fluor® 594 Anti-Glutamine Synthetase antibody [EPR13022(B)] (ab305118)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab302584 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity). Essential for proliferation of fetal skin fibroblasts. -
Involvement in disease
Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD) [MIM:610015]. CSGD is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death. Glutamine is largely absent from affected patients serum, urine and cerebrospinal fluid. -
Sequence similarities
Belongs to the glutamine synthetase family. -
Developmental stage
Expressed during early fetal stages. -
Cellular localization
Cytoplasm. Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 2752 Human
- Omim: 138290 Human
- SwissProt: P15104 Human
- Unigene: 518525 Human
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Alternative names
- cell proliferation-inducing protein 59 antibody
- Cgl2214 antibody
- GLNA antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded human glioma tissue labelling Glutamine Synthetase with ab302584 at 1/100 dilution (5.0 µg/mL) (Green). Cytoplasm staining on human glioma is observed. The section was incubated with ab302584 for 60 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. The nuclear counterstain was DAPI (Blue). Negative control: Primary diluent was used instead of primary antibody. The section was then mounted using Fluoromount®
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 40 mins.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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Immunohistochemical analysis of paraffin-embedded human breast tumor tissue labelling Glutamine Synthetase with ab302584 at 1/100 dilution (5.0 µg/mL) (Green). Cytoplasm staining on human breast tumor is observed. The section was incubated with ab302584 for 60 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. The nuclear counterstain was DAPI (Blue). Negative control: Primary diluent was used instead of primary antibody. The section was then mounted using Fluoromount®
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 40 mins.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab302584 has not yet been referenced specifically in any publications.