RecombinantRabMAb

Recombinant Alexa Fluor® 488 Anti-GSDMD antibody [EPR20859] (ab246711)

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Flow Cytometry - Alexa Fluor® 488 Anti-GSDMD antibody [EPR20859] (ab246711)
  • Alexa Fluor® 488 Anti-GSDMD antibody [EPR20859] (ab246711)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [EPR20859] to GSDMD
  • Suitable for: Flow Cyt
  • Reacts with: Mouse
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Overview

  • Product name

    Alexa Fluor® 488 Anti-GSDMD antibody [EPR20859]
    See all GSDMD primary antibodies

  • Description

    Alexa Fluor® 488 Rabbit monoclonal [EPR20859] to GSDMD

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant full length protein within Mouse GSDMD aa 1 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: Q9D8T2

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Flow Cyt: RAW 264.7 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR20859

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab246711 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Mouse
Application Abreviews Notes
Flow Cyt
1/100.

The cellular localisation of this product has been verified in ICC/IF.
Notes
Flow Cyt
1/100.

The cellular localisation of this product has been verified in ICC/IF.

Target

  • Sequence similarities

    Belongs to the gasdermin family.
  • Information by UniProt

  • Database links

    • Alternative names

      • 1810036L03Rik antibody
      • DF 5L antibody
      • DF5L antibody
      • DFNA 5L antibody
      • DFNA5L antibody
      • FKSG 10 antibody
      • FKSG10 antibody
      • FLJ12150 antibody
      • Gasdermin antibody
      • Gasdermin D antibody
      • Gasdermin domain containing 1 antibody
      • Gasdermin domain containing protein 1 antibody
      • Gasdermin domain-containing protein 1 antibody
      • Gasdermin-D antibody
      • GasderminD antibody
      • GSDMD antibody
      • GSDMD_HUMAN antibody
      • GSDMDC 1 antibody
      • GSDMDC1 antibody
      see all

    Images

    • Flow Cytometry - Alexa Fluor® 488 Anti-GSDMD antibody [EPR20859] (ab246711)
      Flow Cytometry - Alexa Fluor® 488 Anti-GSDMD antibody [EPR20859] (ab246711)

      Overlay histogram showing RAW 264.7 cells stained with ab246711 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal Goat serum to block non-specific protein-protein interactions followed by the antibody (ab246711) (1x 106 cells in 100 µl at 5 µg/ml (1/100 dilution)) for 30 min at 22°C.

      Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor® 488 (ab199091) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

    • Alexa Fluor® 488 Anti-GSDMD antibody [EPR20859] (ab246711)
      Alexa Fluor® 488 Anti-GSDMD antibody [EPR20859] (ab246711)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab246711? Please let us know so that we can cite the reference in this datasheet.

    ab246711 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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